Browsing by Author "Tuncer, B."

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Callus Formation From Isolated Microspore Culture in Radish (Raphanus Sativus L.)
(Pakistan Agricultural Scientists Forum, 2017) Tuncer, B.
In this study, the effects of applications of colchicine on microspore cultures were evaluated in 4 radish (Raphanussativus L.) varieties. For this purpose, 5 different concentrations of colchicine (0, 10, 25, 50, and 75 mg L-1) were applied under dark conditions at 4 degrees C to microspores that had been isolated in Gamborg's medium containing 13% (w/v) sucrose (B5-13). After the application of colchicine the microspores were kept for 1 day at 32.5 degrees C in1/2 x Nitsch and Nitschliquid media supplemented with13% (w/v) sucrose (1/2 NLN-13) at a density of 40.000 microspore mL(-1)and transferred for culturing under dark conditions at 25 degrees C. Colonies of callus were observed in Petri dishes 4-5 weeks after the microspore isolation. In this study, the 50 mg L-1 dose of colchicine was found to be more effective in terms of callus formation. The highest callus formation was achieved from the large round radish (cv. Burkir) (6.63 calli/Petri dish) and the standard small rooted radish (cv. Cherry Belle)(5.73 calli/Petri dish) varieties.
Exploration of Effects of Bleeding Water of Grapevine on in Vitro Germination of Single-Point Endemic Lophanthus Turcicus Seeds
(Scibulcom Ltd, 2017) Turkoglu, N.; Ozudogru, A.; Firat, M.; Keskin, N.; Tuncer, B.
Turkey is highly rich in endemic plant species. Natural spreads of these species are subjected to the risk of extinction through pressures exerted by various factors, especially by anthropogenic factors. Current traditional propagation and conservation methods are rarely sufficient to protect them, thus exploitation of the biotechnological approaches is required, as well. Lophanthus turcicus Dirmenci, Yildiz and Hedge, is a single-point endemic species of Turkey, found in a very narrow area of Van region. Present study explored the efficiency of in vitro germination for this valuable delicate species, and highlighted the promising effects of the artificial medium composed of agarised bleeding water (BW) of grapevine in vivo conditions. In addition to BW medium, also Murashige and Skoog (MS), Gamborg B5, Shenk and Hildebrandt (SH) and White (WH) media were tested for their efficiency. In vitro germination trials were performed both under 16 h photoperiod and in darkness. 90% of germination was achieved when decontaminated seeds were cultured on BW medium, providing a germination rate of 3.5 days and emergence rate of 8.12 days in 16 h photoperiod.
Germination and Emergence of Wild Rhubarb (Rheum Ribes L.) Seeds in Vitro Culture Conditions
(Centenary University, 2019) Tuncer, B.
Rheum ribes L. is a perennial wild plant that is of medical importance as flowers shoots and stalk can be consumed as vegetables. Due to their uncontrolled collection from their natural habitats, it is important to ensure the preservation and sustainability of these species that may be in danger of future extinction. In tissue culture techniques, in vitro micro-propagation studies with seed, in vitro optimum germination mechanism of the species should be known. This study was conducted to determine the in vitro germination and emergence of rhubarb seeds groups (seed tips were cut off or not). These groups were germinated in nutrient mediacombinations, including Murashige and Skoog (MS), Gamborg (B5), White (WH) and Schenk and Hildebrandt (SH) media with or without 200 mg/l gibberellic acid (GA3). As a result of the research, it was determined that the seeds of this species have less severe physiological dormancy and the seed-tip cut applications are more successful in terms of germination and emergence parameters. The highest germination rate (84%) was obtained from MS+200 mg l-1 GA3 nutrient medium, and the best emergence rate (44%) was obtained from WH+200 mg l-1 GA3 medium. © 2019, Centenary University. All rights reserved.
Investigation of the in Vitro Regeneration of Some Medical and Aromatic Wild Plant Species
(Aloki Applied Ecological Research and Forensic inst Ltd, 2017) Tuncer, B.
Ferula orientalis L., Chaerophyllum macrospermum (Spreng.) Fisch. & C.A. Mey., and Eremurus spectabilis M.Bieb are among the medically and aromatically important wild plant species. This study was conducted to obtain in vitro shoot regeneration from different plant segments in these three species. For this purpose, different plant segments (rhizome, leaf, or single-node explants) were cultured in Murashige and Skoog medium with the addition of either 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5-2.0 mg/l) or 2,4-D combined with 6-Benzylaminopurine (BAP) (1.0-2.0 mg/l). For the single-node explants of Ferula orientalis L., the highest shoot number per explant (3.0 shoots/explant) was obtained in the medium with 0.5 mg/l 2,4-D + 2.0 mg/l BAP added. For Chaerophyllum macrospermum (Spreng.) Fisch. & C.A. Mey., the highest shoot number per explant (2.5 shoots/explant) was obtained in the medium with 1.0 mg/l 2,4-D + 2.0 mg/l BAP added. For Eremurus spectabilis M.Bieb., shoot formation was not observed in either of the explant types (leaf or rhizome) in any of the medium combinations. In further studies, the combined applications of 2mg/l or above doses of BAP and lower auxin concentrations may be useful for in vitro shoot regeneration in Ferula orientalis L. and Chaerophyllum macrospermum (Spreng.) Fisch. & C.A. Mey.
Morphology and Utilization of Allium L. Species Used as Herbs in Cheese Around Van Province in Turkey
(int Soc Horticultural Science, 2016) Tuncer, B.; Firat, M.; Yarali, F.; Sarikamis, G.
Allium L. species used in the present study are obtained during floristic field studies in Van province and surrounding regions between 1999-2014 as a result of field observations and investigation of herbarium samples. According to information from the locals of Van district and uplands, 11 Allium species are determined in well known herb cheese of Van province. These species are Allium aucheri Boiss, Allium kharputense Freyn & Sint., Allium pseudoampeloprasum Miscz. ex Grossh., Allium schoenoprasum L., Allium shatakiense Rech. f., Allium szovitsii Regel, Allium vineale L., Allium cardiostemon Fisch. & C.A.Mey., Allium dictyoprasum C.A.Mey. ex Kunth, Allium stipitatum Regel., Allium scorodoprasum L. subsp. rotundum (L.) Stearn. In this study, the entire process from the collection of leaves from their natural habitat till the incorporation of these into herb cheese will be described with original photographs. In addition, detailed and descriptive information about bulbs (shape, diameter, outertunics colour and structure), stem (lenght), leaf (number, width, shape) and floral characteristics (flowering time, umbel shape and diameter, spathe colour and structure, pedicel lenght, flower shape and colour, filament and anther colour) together with general photographs will be provided. Additionally, brief information about the habitat, endemism, phytogeographical regions of these species and map based distribution in Turkey will be presented.