Investigation of the in Vitro Regeneration of Some Medical and Aromatic Wild Plant Species

Abstract

Ferula orientalis L., Chaerophyllum macrospermum (Spreng.) Fisch. & C.A. Mey., and Eremurus spectabilis M.Bieb are among the medically and aromatically important wild plant species. This study was conducted to obtain in vitro shoot regeneration from different plant segments in these three species. For this purpose, different plant segments (rhizome, leaf, or single-node explants) were cultured in Murashige and Skoog medium with the addition of either 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5-2.0 mg/l) or 2,4-D combined with 6-Benzylaminopurine (BAP) (1.0-2.0 mg/l). For the single-node explants of Ferula orientalis L., the highest shoot number per explant (3.0 shoots/explant) was obtained in the medium with 0.5 mg/l 2,4-D + 2.0 mg/l BAP added. For Chaerophyllum macrospermum (Spreng.) Fisch. & C.A. Mey., the highest shoot number per explant (2.5 shoots/explant) was obtained in the medium with 1.0 mg/l 2,4-D + 2.0 mg/l BAP added. For Eremurus spectabilis M.Bieb., shoot formation was not observed in either of the explant types (leaf or rhizome) in any of the medium combinations. In further studies, the combined applications of 2mg/l or above doses of BAP and lower auxin concentrations may be useful for in vitro shoot regeneration in Ferula orientalis L. and Chaerophyllum macrospermum (Spreng.) Fisch. & C.A. Mey.