Browsing by Author "Turkmen, O."

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Determination of Genetic Diversity Among Cumra Melon Genotypes by Issr Markers
(Cukurova Univ, Fac Agriculture, 2012) Turkmen, O.; Sensoy, S.; Erdinc, C.; Paksoy, M.
The present study aimed to determine genetic relationships among 32 melon genotypes collected from Cumra-Konya by molecular markers. Thirty-six polymorphic ISSR markers obtained from 10 primers were used to define the genetic similarity among the melon genotypes by dendrograms or two and three dimensional scaling obtained from Simple Matching distance matrix. The computer program POPGENE was also used to calculate the statistical measures of genetic variation (Nei's gene diversity (H), Shannon's information index (I), and percentage of polymorphic loci). Based on the molecular Simple Matching distance matrix, the most similar genotypes were C15-C22 followed by C23-C26. Of all evaluated genotypes, the most distinct one was C28. The genetic variation estimates for melon genotypes in the present study were high (H = 0.27, I = 0.43 and 100 % polym).
Determination of the Anthracnose (Colletotrichum Lindemuthianum (Sacc. and Magn.) Lambs. Scrib.) Resistance in Some Turkish Bean Genotypes by Artificial Inoculation and Molecular Methods
(Pakistan Agricultural Scientists Forum, 2017) Erdinc, C.; Turkmen, O.; Demir, S.; Sensoy, S.
A total of 123 bean genotypes collected from different regions of Turkey and 7 foreign anthracnose-resistant varieties were evaluated for resistance to anthracnose disease caused by the fungal pathogen Colletotrichum lindemuthianum (Sacc. and Magn.) Lambs. Scrib. Analysis was conducted using artificial inoculation as well as resistance-linked molecular markers. Artificial inoculation was performed in a growth chamber using Race 55. Molecular markers were obtained from SCAR [SAS13 (950 bp, Co-4(2)), SC08 (910 bp, Co-4), SF10 (1072 bp, Co-10), SZ04 (567 bp, Co-6)] and RAPD (OA18(1500) (1500 bp, Co-1(5)) primers associated with resistant genes. Results of artificial inoculation showed that in addition to the 7 foreign varieties, 21 Turkish bean genotypes were anthracnose-resistant, while the remaining 102 Turkish genotypes were not. Moreover, results of molecular-marker screening indicated the presence of one or more amplicons associated with resistant-gene markers (Co-4(2), Co-4, Co-6, Co-10 and Co-1(5)) in the majority of resistant genotypes. Only one accession (G89) had all 5 amplicons, and 6 accessions (G19, G20, G34, G93, G97 and Jaguar) had no amplicons. The present study discovered Turkish bean germplasm of both Andean and Mesoamerican source to have a high level of resistance against anthracnose Race 55. In addition, amplicons connected with several resistance genes were found in this broad bean germplasm. Bean genotypes distinguished in the present study as anthracnose resistant could be utilized in future reproducing programs.
Effect of Capsaicin on Transcription Factors in 3t3-L1 Cell Line
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Berkoz, M.; Yildirim, M.; Arvas, G.; Turkmen, O.; Allahverdiyev, O.
Capsaicin is a spicy ingredient of Capsicum annuum and a lipophilic, crystalline, odorless and colorless alkaloid. Although the effect of capsaicin on adipocyte differentiation is well-known, the role of capsaicin on transcription factors while adipocyte differentiation is not clear. The aim of this study is thus to identify and characterize the transcription factors in the process of adipocyte differentiation after the capsaicin treatment. In this study, concentration of 0, 50, 100, 150, 200 and 250 µM capsaicin were treated to 3T3-L1 pre-adipocytes in cell culture. MTT cell cytotoxicity, cell viability with trypan blue staining, Lactate Dehydrogenase (LDH) enzyme assay, triglyceride content assay, Glycerol-3-Phosphate Dehydrogenase (GPDH) activity, Oil Red O staining and mRNA levels of transcription factors (PPARγ, C/EBPα and SREBP-lc) were investigated in capsaicin induced 3T3L1 preadipocyte cell line. Capsaicin treatment decreased cell population growth of 3T3-L1 preadipocytes, assessed with trypan blue staining, MTT test and rising of LDH release proportion. Capsaicin inhibited GPDH activity and intracellular triglyceride content in 3T3-L1 adipocytes in all treated groups in a dose-dependent manner. Oil Red O staining indicated that capsaicin inhibited adipocyte differentiation in 3T3-L1 adipocytes in all treatment groups. In this study, it was revealed that exposing 3T3-L1 preadipocytes and differentiating postconfluent preadipocytes to different doses of capsaicin decreased PPARγ, C/EBPα and SREBP-1c mRNA levels as compared with their controls without treatment in dose dependent manner. Although, reduction of PPARγ mRNA level was statistical significant, this decrease was not significant in C/EBPα and SREBP-1c mRNA levels. This study demonstrated that capsaicin treatment inhibited the adipogenesis through the down-regulation of transcription factors, especially PPARγ. Alternative mechanisms may involve cell cycle arrest and the induction of apoptosis. Since capsaicin is the main component found in hot pepper, the consumption of hot pepper may contribute to the maintenance of body weight and prevent the development of obesity. © 2015 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effects of Two Different Amf Species on Growth and Nutrient Content of Pepper Seedlings Grown Under Moderate Salt Stress
(Academic Journals, 2008) Turkmen, O.; Sensoy, S.; Demir, S.; Erdinc, C.
This study was carried out to determine the effects of different Arbuscular Mycorrhizal Fungi (AMF) species on the growth and nutrient contents of pepper seedlings (cv. Demre) grown under moderate salt stress. Two different mychorrhizas (Glomus intraradices and Gigaspora margarita) were tested on a growing media containing moderate salt stress (75 ppm NaCl). The study was replicated four times with 8 plants in each replicate. At the end of the study, some nutrients such as P, K, Ca, and Na and plant growth parameters such as shoot height, stem diameter, root length, and dry and fresh weights of shoots and roots were investigated. Saline condition had negative effects on the seedlings. Both AMF species had positive effects on salt tolerance based on the plant growth parameters and nutrient contents. G. intraradices caused better response in seedling development compared to G. margarita, though insignificantly.
Phenotypic and Molecular Genetic Diversityamong Some Turkish Bean Genotypes
(Pakistan Agricultural Scientists Forum, 2017) Erdinc, C.; Turkmen, O.; Dasgan, H. Y.; Sensoy, S.
The genetic relationships among 96common Turkish bean (Phaseolus vulgaris L.) genotypes were investigated based on72 phenotypic characters and molecular ISSR and RAPD markers. Main seed color and predominant secondary seed color were determined as the primary characteristics to distinguish the bean accessions. The mentioned traits, among 72 individual phenotypic traits, explained 58.46 % of phenotypic variation in the first three axes of the principal component analysis (PCA) beside flower duration, plant height, and a number of nodes on the stem. The 21 ISSR primers and 8 RAPD primers having clear and readable band data were also employed, 358 and 116 polymorphic bands were obtained from them, respectively. Polymorphism information content value for ISSR varied between 0.15 and 0.50, while that for RAPD was from 0.31 to 0.48. Among the examined genotypes, molecular genetic relationship determined based on dendrogram obtained by Jaccard distance matrix. Based on the results, the 52% and 48% of the bean genotypes were categorized as Mesoamerican and Andean originated genotypes. In addition, genetic variation values were determined by using Nei and Shannon coefficients and they were highly variable among bean genotypes.
Temperature Changes Under Different Protective Structures at the Late Autumn and Early Spring Periods in Van
(International Society for Horticultural Science, 1999) Akinci, S.; Akinci, I.E.; Karatas, A.; Turkmen, O.
In cold areas protected cultivation has limited due to heating expenses. In microclimates like Van Lake the cost can be decreased. In this study, temperatures of average, maximum and minimum were measured in open (O), low tunnel (L), high tunnel (H), glasshouse (G), low and high tunnel (LH), low tunnel + glasshouse (LG), high tunnel + glasshouse (HG), low tunnel + high tunnel + glasshouse (LHG) between November 1993-April 1994. From these data, the possibilities of protected cultivation and the most suitable growing systems in the area were determined.