Browsing by Author "Turkoglu, Aras"

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Assessment of Genetic Diversity and Population Structure in Local Alfalfa Genotypes Using Ipbs Molecular Markers
(Springer, 2023) Eren, Baris; Keskin, Bilal; Demirel, Fatih; Demirel, Serap; Turkoglu, Aras; Yilmaz, Abdurrahim; Haliloglu, Kamil
iPBS retrotransposon markers, in other words, inter-primer binding site markers based on retrotransposon, have been helpful for the determination of genetic diversity in several plants. The study was evaluated using 10 iPBS molecular markers on the level of genetic diversity and genetic structure of alfalfa genotypes. A total of 280 alleles in 50 alfalfa genotypes (48 local genotypes and 2 commercial varieties) were obtained by 267 polymorphic markers with an average of 28 per locus, ranging from 9 to 41 alleles. The rate of polymorphism of the markers ranged from 86.36 to 100% with an average of 93.71%. The average number of polymorphic bands per marker was detected as 26.7. The mean PIC value and Dice's similarity index were calculated as 0.14 and 0.50 respectively. The results of UPGMA analysis, principal coordinate analysis (PCoA), and STRUCTURE demonstrated that the 50 alfalfa genotypes could be classified into 4 subpopulations, namely the Q1, Q2, Q3, and Q4. The Nei's genetic distances ranged from 0.0121 to 0.0359. iPBS markers and alfalfa genotypes used in this study may be used in studies of alfalfa breeding and germplasm conservation.
Drought-Induced Genomic and Epigenetic Variations in Quinoa Genotypes Revealed by iPBS and CRED-iPBS Marker Systems
(Nature Portfolio, 2025) Turkoglu, Aras; Haliloglu, Kamil; Demirel, Fatih; Demirel, Serap; Isik, Muhammet Islam; Aydin, Adnan; Alipour, Hadi
Drought stress significantly impacts crop productivity, yet its influence on genomic and epigenetic variation in quinoa remains poorly understood. This study aimed to assess DNA damage and cytosine methylation alterations in six quinoa genotypes (Titicaca, Rainbow, Moqu Arrochilla, Cherry Vanilla, China, and White) exposed to five irrigation levels (5%, 10%, 25%, 50%, and 100% field capacity). Genomic changes were evaluated using inter-primer binding site (iPBS) markers, while DNA methylation was analyzed via CRED-iPBS. Results revealed genotype-specific polymorphism and genomic template stability (GTS) responses to irrigation stress. Moqu Arrochilla showed the highest GTS (84.6%) under 5% field capacity, while White exhibited the lowest (35.0%) at 50% field capacity. CRED-iPBS analysis indicated both hyper- and hypomethylation events depending on stress intensity, with China and Moqu Arrochilla genotypes displaying the highest polymorphism rates for MspI (42.9%) and HpaII (39.0%), respectively. These findings highlight the drought-induced genomic and epigenetic variability in quinoa, emphasizing the utility of iPBS and CRED-iPBS techniques for screening stress-responsive genotypes. This work contributes to the understanding of stress adaptation and may inform breeding programs targeting drought tolerance.