Browsing by Author "Uslu, Sema"

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Distribution of Eosinophil Granulocytes and Mast Cells in the Reproductive Tract of Female Goats in the Preimplantation Phase
(Springer, 2009) Karaca, Turan; Yoruk, Mecit; Uslu, Sema; Cetin, Yunus; Uslu, Baris Atalay
Changes in eosinophil granulocytes and mast cells post-insemination may affect conceptus implantation, but information regarding the numbers of such cells in the mammalian reproductive tract is limited. This study investigated the preimplantation distribution of eosinophil granulocytes and mast cells (MCs) in the reproductive tract organs of female goats. Uterus, uterine cervix and uterine tubes samples were obtained at slaughter. Cornu uteri were washed in phosphate buffer solution (each animal contained at least one embryo). Tissues were fixed in 10% neutral buffered formol, Carnoy solution and Mota's fixative (basic lead acetate) for 48 h and embedded in paraffin. Six-micrometre-thick sections were stained with Congo red (for eosinophil granulocytes) and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for MCs). In the uterus, MCs occurred in highest numbers in the myometrium. Higher MC numbers were observed in uterus, uterine and uterine tubes in the preimplantation (experimental) group (cycle synchronised through 7 days intravaginal sponge with 0.3 g P-4) compared with the control group (P < 0.05). Eosinophil granulocyte numbers were significantly higher in the experimental group than in the control group (P < 0.05). These results indicate preimplantation-related changes in numbers of eosinophil granulocytes and MCs in goat reproductive tract organs.
The Effect of Barley Grass on Antioxidant Capacity and Dna Damage in Rat With Renal Failure
(Parlar Scientific Publications (p S P), 2017) Comba, Bahat; Mis, Leyla; Uslu, Sema; Comba, Arzu
This study was aimed to search the effect of barley grass (BG) on the Total Antioxidant (TAS)-Oxidant Status (TOS) and DNA damage (8OHdG) in rat with renal failure. The rats which are used in the study were randomly divided into 4 groups that each of has 8 rats: Control (C) group; injected i.p. with physiological saline once a day for 7 days, GM group; Gentamicin (80 mg/kg/day) was injected i.p. for 7 days, BG group; was given oral BG (250 mg/kg/day) for 4 weeks, GM+BG group was injected gentamicin (80 mg/kg/day) with i.p. for 7 days and BG (250 mg/kg/day) was given by oral for 4 weeks. After from the process of experiment for 4 weeks, blood sample and kidney tissue were taken. The analyses of urea and creatinine were done by autoanalyser; TAS, TOS levels by colorimetric kits; 8OHdG level by ELISA kits in serum. The kidney tissues were examined histologically. In the GM+BG group was statistically decreased levels of urea, creatinine, TOS (p<0.05) and OSI (p<0.01) compared to GM group. In the group of BG was determined the levels of TAS p<0.05 statistically increased other groups. The statistical significance was not found in the level of serum 8OHdG differences between the groups. BG decreased widespread damage to the renal glomerulus and proximal tubulus. As a result, adverse effects of high dose gentamicin application such as kidney damage can be reduced when used barley grass.
Effect of Wheatgrass on Dna Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats
(Soc Chilena Anatomia, 2018) Mis, Leyla; Comba, Bahat; Uslu, Sema; Yeltekin, Asli
This study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 mlikg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001),TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.
Effects of Extract of Green Tea and Ginseng on Pancreatic Beta Cells and Levels of Serum Glucose, Insulin, Cholesterol and Triglycerides in Rats With Experimentally Streptozotocin-Induced Diabetes: a Histochemical and Immunohistochemical Study
(Medwell online, 2010) Karaca, Turan; Yoruk, Mecit; Yoruk, Ibrahim H.; Uslu, Sema
This study investigated the effects of oral administration of extract of green tea (Camellia sinensis) and ginseng (American ginseng-Panax quinquefolium L.), given alone or together, on pancreatic beta-cells, blood glucose, insulin, cholesterol and triglyceride levels in rats with experimental diabetes induced by a single injection of Streptozotocin (STZ) (60 mg kg(-1), i.p.). Fifty adult Wistar Albino rats were used, 10 in each of these five treatment groups: Group A: healthy controls, Group B: STZ-induced diabetes (untreated), Group C: STZ-induced diabetes plus green tea extract (100 mg/kg/daily), Group D: STZ-induced diabetes plus ginseng root (400 mg/kg/daily) and Group E: STZ-induced diabetes plus ginseng root + green tea extracts as before. At the end of the 6 weeks experiment, blood samples were analysed for blood glucose, insulin, cholesterol and triglyceride levels and samples of pancreatic tissue were examined histochemically and immunohistochemically for endocrine islets and beta-cells. Overall, body weight decreased in groups B and C, serum insulin concentrations decreased slightly in groups C-E and total triglyceride levels of blood decreased significantly (p<0.05) in groups B and C compared with control, D and E groups. Histopathological examination showed that degenerative changes in pancreatic beta-cells in STZ-treated rats were minimised to near normal morphology by administration of ginseng (Group D) and ginseng+green tea (Group E) and there was increased intensity of immunohistochemical staining for insulin in these groups. Degeneration of islets of Langerhans beta-cells and weak insulin staining was observed for green tea alone (Group C). These findings demonstrate that ginseng or combined ginseng + green tea decreases blood glucose levels in diabetic rats and increases preservation of P-cells, perhaps by lowering oxidative stress.
Effects of Green Tea and Ginseng on Villus Length and Crypt Depth and on the Distribution of Mast and Goblet Cells in the Small Intestine of Rats With Streptozotocin (stz)-Induced Diabetes
(Univ Philippines Los Banos, College, 2011) Karaca, Turan; Uslu, Sema; Yoruk, Mecit
Diabetes is characterised by abnormally high plasma glucose concentration and causes functional and morphological changes in many organs. This study investigated the effects of green tea extract, ginseng root, and green tea extract plus ginseng root on the micromorphology of and distribution of mast cells and goblet cells in the intestine of rats with streptozotocin (STZ)-induced diabetes. Thirty healthy Wistar albino rats were used, six in each of five groups as follows: Group A (without diabetes): as control, Group B: STZ-induced diabetes without treatment, Group C: STZ-induced diabetes treated with green tea extract alone (100 mg/kg/daily), Group D: STZ-induced diabetes treated with ginseng root alone (400 mg/kg/daily) and Group E: STZ-induced diabetes treated with a combination of green tea extract and ginseng root (dosage as in group C and D). Blood samples were analysed for blood glucose at the end of the six week experiment period. A significant decrease (P<0.05) in serum glucose was observed in treated diabetic groups C, D and E compared with untreated diabetic group B. Mast cell number was significantly lower (P<0.05) in the lamina propria and submucosa of the small intestines, but the number of goblet cells in the duodenum and jejunum was higher in the diabetic groups than in other groups (P<0.05). Goblet cell density in the small intestine tended to be higher in the untreated diabetic group than in the other groups. Green tea extract and ginseng root had no influence on villus height in the duodenum and crypt depth in the ileum of diabetic rats but both had an effect which is characterized by a lower villus height in the jejenum. Green tea extract and ginseng root promoted micromorphology of the small intestine and also caused changes in the distribution of mast and goblet cells of the intestine of diabetic rats.
Effects of Long-Term Release Gnrh Agonist "deslorelin" on Testicular Hsp Expression, Accessory Sex Glands and Testicular Functions in Adult Male Rats
(Elsevier Science inc, 2019) Eski, Funda; Cetin, Nebi; Uslu, Sema; Uslu, Baris Atalay; Sendag, Sait; Yoruk, Mecit; Shakeel, Muhammad
The objective of the present was to determine the effect of long-term release GnRH agonists "deslorelin" on suppression and restoration of testicular and accessory sex glands functions, and expression of HSP in testes of adult male rats. A group of twenty-eight male rats and fifty-six female rats were kept for eleven months. The male rats were subdivided into treatment (n = 18; deslorelin, an analogue of GnRH, 4.7 mg, S.C; six months) and control (n = 10; untreated), and the adult female rats were introduced with either treatment or control male rats at the 2nd, 6th and 11th months post implant insertion. At 6th month of deslorelin implants insertion, six male rats from treatment and five rats from control group were sacrificed. The remaining (twelve treatment and five control) male rats were sacrificed at 11 months. The testicular dimension were measured monthly in both treatment and control rats. The blood samples were collected for testosterone and HSP70 antibody, whereas, the testes and accessory glands were isolated for histological examination at each sacrificial time. The results showed that testicular dimension were significantly lesser in treatment group until 9 months post treatment. HSP70 protein expression was negligible at 6 months in treatment group but its intensity increased in spermatids 11 months of treatment similar to control group. Significantly lower testosterone concentrations with poor semen quality, and smaller litter size were observed in treatment group. The histological picture of accessory sex glands and seminiferous tubules shown a variable integrity in treatment group than control at 6 months implant insertion. In conclusion, the subcutaneous application of 4.7 mg of the GnRH-analogue deslorelin represents a practicable, like in the female rats, method to suppress testicular, accessory sex glands functions, testicular HSP expression and fertility in male rats. Moreover, the suppressive effects of deslorelin, continued until 11th months after removal of the implant. (C) 2019 Elsevier Inc. All rights reserved.
Effects of Nigella Sativa L. and Urtica Dioica L. on the Numbers of Mast Cells in the Thymus, Spleen and Mesenteric Lymph Nodes of Rats With Carbon Tetrachloride Toxicity
(Medwell online, 2007) Yoruk, Mecit; Karaca, Turan; Uslu, Sema
This study investigated the effects of Nigella Sativa L. (NS) and Urtica Dioica L. (UD) on the number of Mast Cells (MCs) in Carbon tetrachloride (CCl4)-treated rats. A total of 35 healthy male Sprague-Dawley rats were divided into 5 equal groups. All groups, expect control group, were injected subcutaneously with 0.8 mL CCl4 per kg body weight twice a week for 45 days and then CCl4 + UD-treated, CCl4 + NS-treated and CCl4 + UD + NS-treated rats received a daily intraperitoneal injection of 0.2 ml, kg(-1) NS or/and 2 mL kg(-1) UD oils for 45 days starting on day 46. Thymus, spleen and mesenteric lymph nodes were sampled, stained with 1% aqueous toluidine blue and examined microscopically. The thymus, spleen and lymph nodes of CCl4- exposed animals contained significantly increased numbers of MCs compared with control group, CCl4 + UD-treated, CCl4 + NS-treated and CCl4 + UD + NS-treated rats (p<0.05). However, when exposed rats were also treated with UD, NS or UD + NS, a decrease in MCs was observed. These results demonstrate that UD and NS decrease the number of MCs induced by CCl4 in the lymphoid tissues of rats.
Immunohistochemical Distribution of Glucagon -, Insulin -, Somatostatin -, Gastrin-, and Serotonin-Containing Cells in the Pancreas of the Van Cat
(Tubitak Scientific & Technological Research Council Turkey, 2014) Karaca, Turan; Kara, Adem; Simsek, Nejdet; Uslu, Sema; Tekiner, Deniz; Yoruk, Mecit
The regional distribution, relative frequency, and appearance of glucagon (A-cell)-, insulin (B-cell)-, somatostatin (D-cell)-, gastrin (G-cell)-, and serotonin (EC-cell)-secreting cells in the endocrine and exocrine pancreas of Van cats were examined using the immunohistochemistry method. Glucagon immunopositive A-cells were principally found in the central region of the islets of Langerhans, while insulin immunopositive B-cells were located in the periphery of the pancreatic islets. Moreover, several A- and B-cells were observed as only single cells or clusters of 2 to 3 immunopositive cells in the exocrine parenchyma and the pancreatic duct epithelium. Somatostatin and gastrin immunopositive reactivities were negligible in the peripheral regions of the pancreatic islets of Langerhans and in the exocrine parenchyma. However, serotonin-immunopositive EC-cells were observed in neither the endocrine islets nor any other sites of the tissue. The existence, regional distribution, and relative frequency of A-, B-, D-, G- and EC-cells in the pancreas of Van cats have been analyzed in this study for the first time. The immunopositivity and distribution of endocrine cells in the Van cat pancreas were determined to be partially different from those of other carnivorous species such as dogs and other cats.
Is Methotrexate-Induced Testicular Damage Preventable Using Nettle Seed Extract: a Histopathological, Immunohistochemical, Biochemical and Spermatological Examination
(Parlar Scientific Publications (p S P), 2018) Uyar, Ahmet; Yaman, Turan; Dogan, Abdulahad; Uslu, Sema; Keles, Omer Faruk; Yener, Zabit; Celik, Ismail
In the study 32 Wistar albino rats were divided into four group as control (Group Control, n=8), methotrexate (Group MTX, n=8), MTX + UDS (Group MTX+UDS, n=8) and Urtica dioica seed extract (UDS) (Group UDS, n=8). After the trial, blood and post-necropsy testicular tissue samples were taken. Histopathological examinations showed that methotrexate had an adverse impact on spermatogenesis by damaging testicles; however, such damages were substantially decreased in the Group MTX+UDS. In the immunohistochemical examinations glutathione peroxidase 1 (GPx1) immunoreactive areas was higher in the Group MTX + UDS compared to the Group MTX. Biochemical examinations revealed that the level of malondialdehyde (MDA), glutathione (GSH), glutathione S-transferase (GST) and catalase (CAT) enzymes levels statistically significantly differenced (p<0.001) in the Group MTX compared to the control, UDS and MTX+UDS groups. There were significant (p<0.05) differences the Group MTX from Group MTX+UDS. such as density, motility, dead-live sperm rate and abnormal sperm rate. Our study results showed that UDS prevented the damage occurred in the testicles according to histopathological, immunohistochemical, biochemical and spermatological findings.
Localisation of Estrogen Receptor Alpha and Progesterone Receptor B in Goat Ovaries During Breeding and Non-Breeding Season
(Kafkas Univ, veteriner Fakultesi dergisi, 2016) Uslu, Sema; Yoruk, Mecit; Mis, Leyla; Comba, Bahat
The main objective of this study was to investigate the localisation of estrogen receptor alpha (ER-alpha) and progesterone receptor B (PR-B) by immunohistochemistry in goat ovaries during in- and out of breeding season. The ovaries obtained from goats slaughtered in breeding season (n=10) and non-breeding season (n=10) were used. From the same animals, blood samples were taken to determine the levels of serum steroid hormones (E-2, P-4). The ER-alpha and PR-B immunohistochemical distributions within the ovaries were determined by the ABC method. In breeding season, the ER-alpha was detected in the germinal epithelium and follicular granulosa cells. The PR-B was determined to concentrate on the corpus luteum (CL) cells. The same receptors were also found to be weak in the theca externa cells of preovulatory follicles. In non-breeding season, the ER-alpha germinal epithelium and smooth muscle cells of certain blood vessels showed a weak positive reaction. The PR-B was positively stained in the germinal epithelium and few stroma cells. It was suggested that the average steroid hormone profiles in breeding (E-2: 11.83 +/- 1.70 pg/mL, P-4: 10.08 +/- 1.58 ng/ml) and non-breeding season (E-2 2.33 +/- 0.85 pg/ml, P-4 0.21 +/- 0.04 ng/ml) correlated well with the localisation intensity of receptors in goat ovaries.
Morflogical and Histometric Studies on Mast Cell Distribution and Heterogeneitiy,present in the Lower Respiratory Tract and in the Lung of Local Duck (Anas Platyrhynchase) and Goose (Anser Anser)
(2011) Uslu, Sema; Yörük, Mecit
Bu çalışma ördek ve kazlarda alt solunum yolları ve akciğerde bulunan mast hücrelerinin dağılımı ve heterojenitesini belirlemek amacıyla yapıldı. Çalışmada 6 adet kaz ve 6 adet ördek kullanıldı. Kanatlı hayvanların solunum sisteminde bulunan trakeya, syrinks, bronş ve akciğerden uygun büyüklükte parçalar alındı. BLA (Basic Kurşun Asetat - Mota), Carnoy, IFAA (Izotonik Formaldehit Asetik Asit)'da tespit edildikten sonra rutin doku takibi yapılarak paraplast ile bloklandı. 6-7 µm kalınlığında alınan kesitler % 0,5'lik toluidine blue ve alcian blue-safranin O kombine boyalarında boyandılar. Toluidine blue ile boyanan kesitlerde 40'lık objektifte 1mm²'lik alandaki mast hücre sayıları belirlendi. Her iki hayvan türünde de tespitlere göre değişmekle beraber incelenen organlardan akciğerde en fazla sayıda mast hücresine rastlandı. Sayısal veriler için en uygun tespitin IFAA olduğu, granül yapısını belirlemek için de en uygun tespitin BLA olduğu saptandı. Alcian blue- safranin O boyamasında ise kazlarda trakeya, syrinks, bronş ve akciğerde SO (+), AB (+) ve miks granüllü mast hücrelerine rastlanırken, ördeklerde trakeyada AB (+) hücreler, syrinks, bronş ve akciğerde de AB (+), SO (+), miks özellikte mast hücreleri görüldü..
Morfological and Histometric Studies on Mast Cell Distribution and Heterogeneity, Present in the Lower Respiratory Tract and in the Lung of Local Duck (Anas Platyrhnchase) and Goose (Anser Anser)
(Kafkas Univ, veteriner Fakultesi dergisi, 2013) Uslu, Sema; Yoruk, Mecit
This study was performed to determine mast cell distribution and heterogeneity present in the lower respiratory tract and in the lung of duck and goose. In this study, 6 ducks and 6 goose were used. Sufficient amount of tissue pieces from trachea, syrinks, bronches and lungs were taken from the choosen poultries. The samples were fixed in BLA (Basic Lead Acetate - Mota), Carnoy, IFAA (Isotonic Formaldehyde Acetic Acid), then, after routin tissue follow up, the samples were blocked with paraplast. Obtained sections (6-7 mu m) were stained with 0.5% toluidine blue and alcian blue - safranin O (AB/SO). Sections stained with toloudine blue were examined under microscope (40 objective) and the number of mast cell in 1 mm(2) were determined. In both animal species; number of mast cells were changable according to fixation technique, with the highest number in lung samples. For numerical results; the most appropriate fixation technique was IFAA and BLA respectively. In AB/SO staining in trachea, syrinks bronches and lungs of geese, mast cells with SO (+), AB (+) and mix granules were obserwed. On the other hand; with the same staining in ducks; AB (+) cells in trachea, AB (+), SO (+) and mix charecteristic mast cells in syrinks, bronch and lungs, were obserwed.
A Morphological and Histometric Study on the Distribution and Heterogeneity of Mast Cells Found in Lungs and Trachea of Van Cats
(Ankara Univ Press, 2015) Uslu, Sema; Yoruk, Mecit
This study was performed to specify the distribution and the heterogeneity of mast cells found in lungs and trachea of Van Cats. Four Van Cats were used in the study. Appropriate sized pieces were taken from the lungs and tracheas of the cats. After BLA (Basic Lead Acetate-Mota) was set, it was blocked with paraplast by doing the routine tissue follow-up. Sections taken in 6-7 mu m thickness were stained with 0,5 % toluidine blue and alcian-blue safranin O combined stain. Numbers of mast cells were specified semi-quantitatively in the sections stained with toluidine blue under lens 40. Among analysed organs, the most mast cells were found in lungs. To specify the granule construction, BLA detection was determined to be suitable. In the stain of Alcian-blue safranin O, SO (+), AB (+) granulose mast cells were found; mast cells that have mixed granules were not found.
Postpartum Uterus Involution Observed by Real-Time Ultrasound Scanning and Vaginal Cytology in Van Cats
(Sage Publications Ltd, 2016) Sendag, Sait; Alan, Muhammet; Eski, Funda; Uslu, Sema; Uslu, Baris A.; Wehrend, Axel
Objectives The objective was to investigate postpartum uterus involution by real-time ultrasonography and vaginal cytology in Van cats. Methods This study included 15 healthy Van cats belonging to the Van Cat Research Centre (Yuzuncu Yil University, Van, Turkey). Starting 24 h postpartum, ultrasonographic measurements were performed on the placental and interplacental uterine horn regions every day. Decreases in the diameters and uterine content were considered as criteria for uterine involution. Vaginal discharge samples were collected every day for 4 weeks postpartum. The smears were stained with Papanicolaou stain. Results The average diameters of placental and interplacental regions (IPRs) in the uterine horns were 3.12 +/- 0.29 cm and 2.36 +/- 0.43 cm, respectively, at 24 h postpartum. Placental regions (PRs) shrank faster than IPRs. At 48 h postpartum, it became difficult to distinguish PRs from IPRs in the uterine horns. The uterine horns could be seen in the abdominal cavity up to 5.60 +/- 0.99 days postpartum. The mean of the last assessable diameter of the uterine horns from days 4 to 7 in all cats was 0.49 +/- 0.07 cm. The vaginal epithelial cells appeared to be under the effect of oestrogen for 4 weeks postpartum. Conclusions and relevance The morphological involution of the uterus completes, to a large extent, within the first 48 h postpartum in Van cats. A more detailed hormonal analysis would contribute greatly to the understanding of the physiological processes involved in this period. Although postpartum involution appeared complete by 5.60 +/- 0.99 days after parturition in Van cats, histological verification of this finding is needed.