Browsing by Author "Usta, A."

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The Effect of Thymoquinone Treatment on Total Oxidant and Total Antioxidant Level in Experimental Diabetic Rats
(Ataturk Universitesi, 2018) Usta, A.; Dede, S.; Çetin, S.
In this study, the effects of thymoquinone implementation to total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were investigated in liver and kidney tissues of diabetic rats induced with STZ. The 24 male Wistar-Albino rats, weighing between 200-250 g were used as material. Rats were divided into four groups, each consisting of six rats; control (C), thymoquinone (TQ), diabetes (D) and diabetes+ thymoquinone (DT) groups. To induce diabetes, a single dose of 45mg/kg streptozotocin (STZ) were administered intraperitoneally (ip) to the rats in groups D and DT. TQ was administered to the rats in DT and TQ groups as 30 mg/kg/21 days by oral gavage. The values of TAS and TOS were measured in liver and kidney tissues at the end of the study. OSI was calculated according to TAS and TOS levels. TAS value in liver tissue was found to be the lowest in the diabetes group (P≤0.05). It was noticed that there was no difference between K and TQ groups with DT group. No difference was detected among groups in terms of liver TOS. It was determined that the OSI in the liver increased in all groups compared to the control (P≤0.05), the highest value was determined in group D, it approached to the control by partially decrease after the application of TQ. While TAS and TOS levels in kidney tissue were found to be the highest in the TQ group and the lowest in the diabetic group (P≤0.05), there was no difference between the other groups. There was no significant difference among the groups in the kidney OSI. As a result, it was determined that TOS, TAS values in liver and kidney tissues of experimental diabetic rats were affected from the TQ application and the OSI value in the liver of the STZ treated groups approached that of the control group, after TQ application. © 2016 Ataturk Universitesi. All rights reserved.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride
(Humana Press inc, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.
Prolonged exposure to high doses of fluoride causes chronic poisoning called fluorosis, which affects many tissues and causes serious health problems. This study was planned to investigate the apoptotic, autophagic, and necrotic molecular pathways of fluoride. Sodium fluoride (NaF) was administered to normal rat kidney epithelial (NRK-52E) cells. The NaF IC50 value was determined using the MTT assay. The expression of the genes in the autophagic, apoptotic, and necrotic pathways was determined by real-time PCR. It was determined that there were significant changes in NaF-induced molecular pathways depending on the time. There were no increases in apoptotic and necrotic pathway markers except for Atg3, an autophagy gene, at the 3rd and the 12th hours. However, there was an induction in all cell death signaling pathways at 24 h. The molecular mechanisms demonstrated NaF-induced cellular death in the NRK-52E cell line. It was concluded that these molecular mechanisms were activated with NaF, and different mechanisms accelerated the cellular death at the 24th hour.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride (Nov, 2020, 10.1007/S12011-020-02491-3)
(Springernature, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.
Serum Protein Fraction Profile in Sheep Infected With Sarcoptes Ovis
(Ataturk Universitesi, 2020) Çetin, S.; Usta, A.; Ekici, P.; Dede, S.; Yüksek, V.
Scabies is an ectoparasitic infestation that is rapidly transmitted to sheep of any age. The present study was conducted to identify the significance of serum protein fractions in the diagnosis and treatment of animals infected by Sarcoptes ovis. The study group included 1-4 years old 18 Akkaraman sheeps with excessive itching and molting and infected with natural Sarcoptes ovis scabies and the control group included 1-4 years old 18 healthy sheeps, a total of 36 sheeps were included in the present study. Serum protein fractions were determined with the cellulose-acetate electrophoresis method in collected blood samples. There were no significant differences between total protein and α-2 globulin serum protein concentrations in the study and control groups. Although α-1, β-globulin and albumin concentrations were statistically higher in the patient group, gamma globulin levels decreased in the patient group (P<0.05). While the percentage (%) g protein did not differ based on α-2 globulin when compared to control, α-1, beta globulin, albumin and A/G ratio statistically increased in the patient group when compared to the control (P<0.05). There was a significant decrease in gamma globulins in the patient group when compared to the control (P<0.01). It was concluded that monitoring the serum protein parameters might be significant in the follow-up and treatment of scabies in sheep. © 2020 Ataturk Universitesi. All rights reserved.