Browsing by Author "Vural, Mehmet Emin"

Now showing 1 - 4 of 4

A Mitochondrial Dna-Based Molecular Phylogenetics Study of the Mahalli Goat as a New Animal Genetic Resource in Southern Anatolia in Turkey
(Agricultural Research Communication Centre, 2024) Kiraz, Selahaddin; Koncaguel, Seyrani; Vural, Mehmet Emin; Koyun, Hasan
Background: The Karacada & gbreve; Mountains are of extinct volcanic origin and cover a large area in the triangle between Diyarbak & imath;r (Center, Nar and Ergani districts), & Scedil;anl & imath;lurfa (Siverek and Viransehir districts) and Mardin (Derik district). Agricultural activities are limited due to the stony structure of the region; therefore, small animal husbandry is intensively practiced in the villages of the region. Mahalli goats, which are morphologically new domestic goats, are distinguished from other domestic goats by their spiral horn structure. They are not only resistant to cold weather conditions, but also resistant to diseases. Therefore, present study aimed to determine the genetic diversity and phylogenetic relationships of Mahalli goats in Diyarbak & imath;r region. Methods: The study was carried out at & Scedil;anl & imath;urfa, Harran University of Agricultural Faculty, Laboratory of Animal Biotechnology and Molecular Genetics, in 2019-2020. Out of 24 Mahalli goats, forward and reverse primers were used to amplify the 598 bp region over the mtDNA D-loop region encompassing the 481 bp most variable region (HVR1). Analysis of goat D-loop sequences revealed 481 regions of 0.378 G+C, 97 polymorphic regions and 19 haplotypes. The phylogenetic analysis of Mahalli goats was carried out using molecular genetic techniques. Result: As a result of data analysis, both haplotype diversity (HD) and nucleotide diversity (ND) were calculated to be 1.000 +/- 0.0039 and 0.0375 +/- 0.00209, respectively. The genetic distance between the haplotypes of Mahalli goat ranged from 0.003 to 0.087 and the genetic distance between the haplotypes of Mahalli goat and other domestic goat breeds in Turkey ranged from 0.016 to 0.027. All 19 haplotypes used in this study were represented in the lineage A phylogenetic tree.
Molecular Genetic Analysis With Rapd-Pcr Method of Zom Sheep
(2014) Vural, Mehmet Emin; Bingöl, Mehmet; Kiraz, Selahaddin
Bu çalışmada 2012-2014 yılları arasında Diyarbakır İli Karacadağ yöresinde, farklı sürülerde bulunan 18 baş zom koyunundan alınan kan örnekleri kullanılmıştır. Harran Üniversitesi Ziraat Fakültesi Hayvansal Biyoteknoloji Labaratuvarı'nda izole edilen DNA örnekleri 8 adet RAPD primeri ile kullanılarak işlenmiş ve RAPD-PCR bantları elde edilmiştir. Agaroz- Jel fotoğrafları incelenmiş ve elde edilen bantlar ile bantların var (1) ve yok (0) olmasına göre oluşturulan veri matrisi, PopGen32 paket programı kullanılarak, değerlendirilmiştir. Çalışmada 300-3000 bç aralığında 899 adet bant elde edilmiştir. Kullanılan 8 adet primer ile toplamda 54 lokus tespit edilmiş olup, bu lokusların 15'i polimorfik 39'u monomorfik bulunmuş, polimorfizm oranı ise % 27.8 olarak hesaplanmıştır. OPA-19 primeri ile tüm lokuslar polimorfik bulunurken, OPE-02 ve OPE-06 primerlerinde ise polimorfizm tespit edilememiştir. Genetik uzaklık, en yüksek 3 – 6 (0.189) nolu örnekler arasında, en düşük ise 4 - 5 (0.019) nolu örnekler arasında tespit edilmiştir. Genetik benzerlik değeri en az 3 - 6 nolu örnekler arasında (0.811), en çok ise 4 - 5 nolu örnekler arasında (0.981) bulunmuştur. Ortalama heterozigotluk 0.1253 olarak bulunmuştur. Sonuç olarak RAPD-PCR yöntemiyle Zom koyunlarında polimorfizm, genetik benzerlik, genetik uzaklık ve heterozigotluk gibi bazı genetik parametreler hesaplanarak DNA seviyesinde tanımlayıcı ön bilgiler elde edilmiş, bireylerin heterojen bir yapıda olduğu ve bu genetik çeşitliliğin ıslah amaçlı kullanım potansiyeline sahip olduğu söylenebilir.
Morphological Characteristics of Zom Sheep
(Kafkas Univ, veteriner Fakultesi dergisi, 2012) Koncagul, Seyrani; Akca, Nalan; Vural, Mehmet Emin; Karatas, Ahmet; Bingol, Mehmet
This research was a descriptive study and carried out on Zom sheep raised in the mount Karacadag province in Diyarbakir, Turkey. The study was conducted to determine some morphological characteristics of the Zom sheep according to different age and sex groups. A total of 16 males and 195 females were measured. Overall means and standard errors of some characters of rams and ewes were: live weight 65.0 +/- 2.0 and 46.0 +/- 1.25 kg, wither height 76.2 +/- 1.57 and 67.9 +/- 0.94 cm, height at rump 75.5 +/- 0.83 and 67.9 +/- 0.50 cm, body length 66.1 +/- 0.91 and 60.2 +/- 0.55 cm, heart girth circumference 99.8 +/- 1.73 and 94.7 +/- 1.04 cm, chest depth 34.4 +/- 0.59 and 29.9 +/- 0.36 cm, chest width 21.1 +/- 0.49 and 18.2 +/- 0.30 cm, respectively. The overall results of this study show that the Zom sheep have some similarities with Karakas, Awassi and Akkaraman sheep according to morphological characteristics.
Reproductive Performance of Ewes and Growth Characteristics of Lambs in Zom Sheep Reared in Karacadag District
(Kafkas Univ, veteriner Fakultesi dergisi, 2013) Koncagul, Seyrani; Vural, Mehmet Emin; Karatas, Ahmet; Akca, Nalan; Bingol, Mehmet
This is the first study and report in which some ewe reproductive and lamb growth performances of Zom sheep were investigated and described in Turkey. The research was carried out on three different flocks randomly chosen among the Zom sheep flocks in the district of Karacadag surrounded by Diyarbakir, Sanliurfa and Mardin provinces, and lasted for two years, 2010 and 2011. The data consisted of 242 and 254 heads of lamb born from 226 and 214 heads of ewe in 2010 and 2011, respectively. Lambing rate (LR), twinning rate (TW), fecundity (FEC) and litter size (LITS) were found as 94%, 17%, 1.09 and 1.17 on average, respectively. There was no significant difference among flocks within years or among years within flocks in terms of ewe reproductive performance, except for the flock 2 in terms of TW. Average means of the birth weight (BWT), weaning weight (WWT) at 90th day and 6th month live weight (SMLW) were 4.00 kg and 22.06 kg, 36.11 kg, respectively. The effects of age of dam, type of birth and sex of lambs were significant on BWT, WWT and SMLW (P<0.05). Besides the WWT was also significantly affected by the year (P<0.05), every 1 kg increase in BWT caused 2.02 +/- 0.281 kg increase on average in WWT of lambs (P<0.01). In addition, it was observed that the havier lambs at birth were havier in terms of SMLW (P<0.01). The average daily weight gain (ADWG1) and survival rate (SR) of lambs at weaning were 200 g/day and 90%, respectively.