Browsing by Author "Wells, Kenny J."

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    Cpg Motif-Based Adjuvant as a Replacement for Freund's Complete Adjuvant in a Recombinant Lhrh Vaccine
    (Elsevier Sci Ltd, 2008) Conforti, Valeria A.; De Avila, David M.; Curnmings, Nada S.; Zanella, Ricardo; Wells, Kenny J.; Uelker, Hasan; Reeves, Jerry J.
    This study compared: (1) Freund's complete adjuvant and CpG oligodeoxynucleotide (ODN) 2006 in water-in-oil emulsion as adjuvants; and (2) increasing doses of a recombinant ovalbumin-LHRH (ova-LHRH) fusion protein as an antigen for a contraceptive vaccine. Treatment groups (n = 8 heifers/group) were: one untreated control group; five groups receiving CpG ODN with different doses of ova-LHRH (1.5; 2.3; 3.4; 5.1; and 7.6 mg); and one group receiving 3.4 mg ova-LHRH in Freund's. Heifers were immunized at weeks 0 and 14. Ail vaccine treatments caused gonadal regression and estrus suppression. CpG ODN is a suitable replacement for Freund's for LHRH immunization. (c) 2007 Elsevier Ltd. All rights reserved.
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    The Effectiveness of a Cpg Motif-Based Adjuvant (Cpg Odn 2006) for Lhrh Immunization
    (Elsevier Sci Ltd, 2007) Conforti, Valeria A.; de Avila, David M.; Cummings, Nada S.; Wells, Kenny J.; Uelker, Hasan; Reeves, Jeffy J.
    A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum. Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization. (C) 2007 Elsevier Ltd. All rights reserved.