Browsing by Author "Yörük, M."
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Article The Effect of Plantago (Plantago Lanceolata) Added Into Diets at Specific Proportions Upon Distribution of Mast Cells of Digestive System Organs in Male Quails(Ataturk Universitesi, 2016) Uslu, S.; Temur, C.; Yörük, M.This research was performed to determine the effect of plantago (Plantago Lanceolate) added 0, 1, 3, 5% into diet groups (n=6 each) on the number of mast cells in some organs of the digestive system in male quails (n=24, in total). In digestive system of quails, the appropriate pieces of tissue samples were taken from the proventriculus, duodenum, jejunum and ileum, and fixed in Mota's basic lead asetat solution for 24 h and after histological tissue processing, embebbed in paraplast. The sections of 6 μ in thickness were stained with toludine blue (0.5%) at pH 0.5. When distributions of mast cells in the lamina propria, submucosa, tunica muscularis plus tunica serosa were examined, it was observed that most of the mast cells were located within the submucosal layer. In the organs examined, the highest number of mast cells were found in the proventriculus. It was determinded that plantago added into diets increased the number of mast cells in male quails.Article Investigation of the Effect of Gnrh (Deslorelin) on the Histochemical Structure of Salivary Glands in Rats(Universidad de la Frontera, 2019) Uslu, S.; Çakmak, G.; Yörük, M.This study was planned to determine the histochemical alterations of the submandibular gland by implantation of long-term GnRH (deslorelin 4.7 mg). Eighteen Wistar albino rats were used in the study. Alcian blue (AB; pH: 2.5), periodic acid–Schiff (PAS) staining was performed to determine the microscopic structure and histochemical structure of the GI submandibular gland. The Avidin–Biotin Complex (ABC) method was used to determine the immunohistochemical reactivity of lectin. After GnRH implantation, the organs were examined and atrophies were observed in organs. In the group in which the implants were removed, it was determined that there was no atrophy; organ structures and microscopic examination were similar to the control group. At the end of the study, submandibular gland was fixed in 10 % buffered formaldehyde. In three groups, PAS and AB histochemical staining revealed similar reactions. Immunohistochemically, lectin activity was found to react positively. © 2019, Universidad de la Frontera. All rights reserved.