Browsing by Author "Yenilmez, Ayse"

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Effects of Emodin and Metformin on Biochemical, Histological and Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats
(Polish Society Magnesium Research, 2023) Bati, Bedia; Yildirim, Serkan; Celik, Ismail; Kaptaner, Burak; Huyut, Zubeyir; Yenilmez, Ayse; Demir, Abdulbaki
Diabetes mellitus (DM) is a lifelong metabolic disease with a high mortality rate, which reduces the quality of life of many people worldwide. Metformin is a hypoglycemic drug usually used to treat type 2 diabetes. Emodin has various protective effects and is widely used in holistic medicine. The aim of this study was to investigate the effects of emodin, metformin and the emodin + metformin combination in liver and kidney tissues of rats with diabetes induced by streptozotocin (STZ). For this purpose, 5 groups: I - Group Control (CG), II - Group STZ control (SC), III - Group STZ + metformin 250 mg kg-1 (SM), IV - Group STZ + emodin 40 mg kg-1 (SE) and V - Group STZ + metformin 250 mg kg-1 + emodin 40 mg kg-1 (SME) were formed with 7 rats in each group. Liver, kidney and blood samples were taken at the end of the experiment. In the study, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), gluta-thione-S-transferase (GST), glutathione reductase (GR), reduced glutathione (GSH) and malond-ialdehyde (MDA) levels in liver and kidney tissues were analyzed. In addition, histopathology and immunohistochemical and serum toxicity biomarkers (AST, ALT, BUN and CREA) were analyzed. According to the experimental results, there was an increase in serum biomarker values in the SC group compared to the control group (p values for AST, ALT, CREA and BUN were p=0.005, p=0.001, p=0.006, p=0.001, respectively). Some antioxidant parameter values increased in SM, SE and SME groups compared to CG and SC groups (p=0.001 for GR in liver tissue and p=0.032 for GPX in kidney tissue). Histopathologic and immunohistochemical obser-vations also supported biochemical parameter findings. In conclusion, oxidative stress parame- ters in liver and kidney tissue, serum samples, histopathologic and immunologic findings sug- gest that metformin, emodin and the metformin + emodin combination have positive effects on nephrotoxic and hepatotoxic side effects.
Preliminary Phytochemical Analysis and DNA Protective Activities of Ephedra Major and Ephedra Equisetina Species
(Pamukkale University, 2025) Demirkuş, Nasip; Ünal, Murat; Bati, Bedia; Yeskendirov, Farkhad; Mukhitdinov, Nashtay; Yenilmez, Ayse
Worldwide, the Ephedra plant is one of the oldest medicinal herbs used in traditional alternative medicine. The medicinal varieties are utilized as stimulants and antiasthmatic agents for the treatment of colds, bronchial asthma, cough, fever, influenza, headache, edema, and allergies. In our study, it was aimed to determine the preliminary phytochemical composition of Ephedra major and Ephedra equisetina species belonging to the Ephedra genus and to determine their antioxidant and DNA protective activity. Ephedra major is known to contain components such as flavonoids and tannins with a moderate ephedrine content and has been traditionally used in the treatment of respiratory diseases. Ephedra equisetina, on the other hand, contains higher concentrations of ephedrine and other bioactive compounds and is widely used in traditional medicine for the treatment of asthma, bronchitis, and allergic reactions. When we look at the study findings, the total phenolic and flavonoid substance amounts in both plant species were determined at close levels in both tests, and it was seen that they contained phenolic and flavonoid substances and had antioxidant activity and DNA protection effect. In addition, it was observed that there was a DNA protection effect at a concentration of 0.5 mg/mL in the presence of Fenton in the medium.