Browsing by Author "Yilmaz, A. B."

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First Detection of Ehrlichia Chaffeensis, Ehrlichia Canis, and Anaplasma Ovis in Rhipicephalus Bursa Ticks Collected From Sheep, Turkey
(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2024) Ayan, A.; Celik, B. Aslan; Celik, O. Y.; Kilinc, O. Orunc; Akyildiz, G.; Yilmaz, A. B.; Babaoglu, A. R.
Anaplasmosis and ehrlichiosis are important tick -borne rickettsial diseases of medical and veterinary importance that cause economic losses in livestock. In this study, the prevalence of Anaplasma ovis, Ehrlichia canis and Ehrlichia chaffeensis was investigated in ticks collected from sheep in various farms in Van province, which is located in the Eastern Anatolian Region of Turkey. The ticks used in this study were collected by random sampling in 26 family farm business in 13 districts of Van province. A total of 688 ticks were collected from 88 sheep and 88 tick pools were created. All ticks identified morphologically as Rhipicephalus bursa. Phylogenetic analysis of Chaperonin and 16S rRNA gene sequences confirmed A. ovis, E. canis and E. chaffeensis in this study. Of the 88 tick pools tested, 28.41% (25/88) were positive for at least one pathogen. Anaplasma DNA was detected in five of the 88 pools (5.68%), E. canis DNA was detected in 19 of the 88 pools (21.59%), and E. chaffeensis DNA was detected in one of the 88 pools (1.14%) of R. bursa ticks. To our knowledge, this is the first report describing the presence of A. ovis, E. canis, and E. chaffeensis in R. bursa ticks collected from sheep in Turkey. Further studies are needed to investigate other co -infections in sheep in Turkey.
Investigation of Serum Amyloid A, Haptoglobin and Fibrinogen Levels in Sheep Infected With Fasciola Hepatica
(Journal Livestock Science, 2019) Denizhan, V.; Kozat, S.; Yilmaz, A. B.
The present study aimed to determine the changes in serum haptoglobin, serum amyloid A and fibrinogen levels in pathological disorders of sheep liver with Fasciola hepatica. Sixty sheep (50 with fascioliasis and 10 healthy) were used in the present study. Statistical analysis results demonstrated that SAA, Hp and fibrinogen concentration levels of the sheep infected with Fasciola hepatica were higher when compared to SAA, Hp and fibrinogen concentration levels in healthy sheep (P <0.05). While ALT, AST and GGT values of sheep infected with Fasciola hepatica were found to be higher than those of the healthy sheep, serum TP, ALB and glucose levels were lower. In statistical analysis, it was found that WBC, Neu and Eo values of sheep infected with Fasciola hepatica were higher when compared to WBC, Neu and Eo values in the control group. In conclusion, serum haptoglobin, serum amyloid A, and fibrinogen levels were significantly higher in sheep infected with Fasciola hepatica when compared to the healthy group. Due to the fact that increases in serum haptoglobin, serum amyloid A and fibrinogen levels in sheep with fasciolosis were similar to those observed in liver disorders, it was suggested that these parameters could be useful in determination of the liver damage levels and prognosis and in studies on liver disorders.
Molecular Detection and Subtype Distribution of Blastocystis Sp. From Shelter Dogs and Cats in Van, Turkey: First Report of St10 in Cats and St1, St10 and St30 in Dogs
(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2024) Ayan, A.; Celik, B. Aslan; Celik, O. Y.; Yilmaz, A. B.; Kilinc, O. Orunc; Ayan, O. Oktay
Blastocystis is an intestinal protist commonly found in humans and many different animal species. It is probably the most common enteric parasite with an estimated one billion infections worldwide. The fecal materials for this study were collected from 100 cats and 200 dogs different age and sex in shelter in Van, Turkey. DNA extraction, PCR amplification and sequence analysis were performed on the fecal samples. As a result, a prevalence of 1% (1/100) in cats and 1.5% (3/200) in dogs was detected. The prevalence was higher in both cats and dogs, in age groups younger than one year and in females according to gender. Sequence analysis revealed Blastocystis sp. ST10 in cats and Blastocystis sp. ST1, ST10 and ST30 in dogs. The sequences obtained were deposited in Genbank. In conclusion, stray cats and dogs may be a source of infection for other cats and dogs, and the detection of zoonotic ST1 in dogs suggests that dogs may be a reservoir for human infection.