Browsing by Author "Yoruk, M."

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Age Related Distribution of Mast Cells in the Trachea and Lung of Chicken
(indian veterinary Journal, 2006) Karaca, T.; Yoruk, M.; Simsek, N.
Age-Related Changes in the Number of Mast Cells in the Avian Lymphoid Organs
(Wiley-blackwell Publishing, inc, 2006) Karaca, T.; Yoruk, M.; Uslu, S.
The distribution of mast cells (MCs) was studied in the lymphoid organs (thymus, bursa of Fabricius and spleen) of 0-, 7-, 21-, 30- and 120-day-old chickens, using light microscopic histochemical techniques. Tissues samples were obtained under deep anaesthesia from animals in five groups. Tissues were fixed in Mota's fixative (basic lead acetate) for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with toluidine blue in 0.5% aqueous solution at pH 1.0 for 5 min and Alcian blue/Safranine at pH 1.42 for 30 min. MCs were found in the organs, mostly associated with sinuses and blood vessels. A large increase in MCs was observed in both thymus and spleen of 21-day-old chickens compared with 0-, 7-, 30- and 120-day-old chickens. However, in the bursa of Fabricius, numbers of MCs were significantly higher in the 7-day-old group compared with other age groups. Safranine-positive MCs were not observed in all organs and age groups. These results showed age-related changes in the number of MCs in avian lymphoid tissues.
Changes in Testicular Development, Ultrasonographic and Histological Appearance of the Testis in Buck Kids Immunized Against Lhrh Using Recombinant Lhrh Fusion Protein
(Wiley-blackwell Publishing, inc, 2009) Ulker, H.; Kucuk, M.; Yilmaz, A.; Yoruk, M.; Arslan, L.; deAvila, D. M.; Reeves, J. J.
This study was designed to evaluate the effectiveness of recombinant Ovalbumin-LHRL (OL) immunization on changes in testicular size, histological appearance and testosterone production in buck kids. Thirty native buck kids at 18 weeks of age were divided into three groups, control (n = 10), immunization (n = 10) and castration (n = 10) groups. Immunized animals received OL protein generated by recombinant DNA technology. Ultrasonographic and histological examinations of the testes were performed. Animals were slaughtered at 44 weeks of age. Semen and epididymides were evaluated for the presence of sperm cells. Immunized animals generated anti-LHRH antibodies. Testosterone production, testicular and accessory glands development and sperm production were suppressed in the immunized animals (p < 0.01). Semineferous tubule diameters decreased (p < 0.01), basal membrane of the tubule was thickened and hyalinized in immunized kids. Immunization affected ultrasonographic appearance of the testes drastically. While testes of control animals gained their normal ultrasonographic appearance as the age increased, immunized animals had uniform hypoechogenic testicular structure as observed at 18 weeks of age until slaughter. Simultaneous histological and ultrasonographic evaluations indicated that the changes in testicular histology could partly be monitored via ultrasonographic imaging; nevertheless, it is difficult to claim that ultrasonographic image reflects the exact changes in such instances. In conclusion, these results indicate that recombinant OL fusion protein is effective in immunocastration in buck kids and has a potential to be used as an alternative to physical castration. Further researches should be conducted to help assessing reproductive status of testes from ultrasound images.
Distribution and Heterogeneity of Mast Cells in Female Reproductive Tract and Ovary on Different Days of the Oestrus Cycle in Angora Goats
(Wiley-blackwell, 2008) Karaca, T.; Arikan, S.; Kalender, H.; Yoruk, M.
The physiological distribution of mast cells (MCs) in the reproductive tract and ovary of 12 Angora goats was determined using light microscopic histochemical techniques. Uterus (corpus uteri and cornu uteri), uterine cervix, uterine tubes (isthmus and ampulla) and ovary samples were obtained by laparatomy from groups of animals during metoestrus, dioestrus and proestrus (days 5, 10 and 16 of the oestrous cycle). Tissues were fixed in Mota's fixative (basic lead acetate) for 48 h and embedded in paraffin. Six-micrometre-thick sections were stained with toluidine blue in 1% aqueous solution at pH 1.0 for 5 min and alcian blue-Safranin at pH 1.0 for 30 min. MCs were generally associated with blood vessels in all reproductive organs. In the uterus, they were concentrated mainly in the close of the uterine gland and deep stroma in the endometrium. Higher MC numbers were observed by toluidine blue staining in the uterus, uterine cervix and uterine tubes on days 10 (corpus uterine: 4.7 +/- 3.8 and cornu uterine: 4.9 +/- 3.5) and 16 (corpus uterine: 5.9 +/- 4.5 and cornu uterine: 5.4 +/- 2.4) of the oestrous cycle compared with day 5 (p < 0.05). Mast cells were not observed in the follicles, the corpus luteum and the underside of the surface epithelium of the ovarian cortex, but were observed in the interstitial cortical stroma and the ovarian medulla. In the ovary, MC numbers were significantly higher on day 16 of the oestrous cycle (cortex: 3.4 +/- 2.4 and medulla: 5.7 +/- 4.5, p < 0.05). Safranin-positive connective tissue MCs were not observed in the uterine tube on any occasion. These results indicate oestrous cycle-related changes in the number and location of MCs in goat reproductive organs.
Distribution and Quantitative Patterns of T Lymphocytes in the Female Reproductive Tract and Ovary Throughout the Oestrus Cycle of Angora Goats
(2007) Karaca, T.; Arikan, S.; Kalender, H.; Yoruk, M.
The present study was designed to evaluate the distribution of alpha-naphthyl acetate esterase (ANAE) positive T lymphocytes in the female reproductive tract and ovary throughout the estrous cycle of Angora Goats. Tissue samples were collected on days 5, 10 and 16 of the oestrus cycle and then fixed in formol-sucrose fixative (pH 6.8) and stored for 22 hours at +4°C. The samples were then additionally fixed in Holtz' solution under the same conditions they were in the first fixation. Cryostat sections of 8 μm thickness were stained for alpha naphthly acetate esterase activity at pH 6.4. The ANAE positive T lymphocytes were mainly located in the epithelium, lamina propria and around blood vessels in other region of connective tissues. Density of ANAE positive T lymphocytes in the ovarian and uterine tissues was highest on day 10 and 16 respectively. It was concluded that the estrous cycle may have been responsible for variations in the distribution of ANAE positive T lymphocytes in goat ovarian and reproductive tract tissues.
The Effect of Royal Jelly on Cd3+, Cd5+, Cd45+ T-Cell and Cd68+ Cell Distribution in the Colon of Rats With Acetic Acid-Induced Colitis
(Elsevier Espana Slu, 2012) Karaca, T.; Simsek, N.; Uslu, S.; Kalkan, Y.; Can, I.; Kara, A.; Yoruk, M.
Background: Traditional medicines and health supplements have historically been used to treat many illnesses but most of them have not been evaluated objectively to prove their efficacy. We have been investigating the effects of royal jelly (RJ) supplements on acetic acid-induced colitis on the distribution of CD3(+), CD5(+), CD45(+) T-cell and CD68(+) cells in rats. Methods: The rats were divided into four equal groups: control group, royal jelly-treated (RJ - 150 mg kg(-1) body weight), acetic acid-treated (colitis) and acetic acid-treated (colitis) + royal jelly (CRJ - 150 mg kg(-1) body weight). Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 mL kg(-1)). Colon samples were obtained under deep anaesthesia from animals in four groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Results: The proliferative response of CD3(+) and CD45(+) T cells stimulated with colitis was affected by colitis treated with RJ. No differences were found in CD5(+) T cells and CD68(+) macrophages in the colitis treated with RJ. Conclusions: This study has shown that RJ has anti-inflammatory and cell regeneration effect in the colon of rats with acetic acid induced colitis. (c) 2011 SEICAP. Published by Elsevier Espana, S.L. All rights reserved.
Effect of Royal Jelly on Experimental Colitis Induced by Acetic Acid and Alteration of Mast Cell Distribution in the Colon of Rats
(Pagepress Publ, 2010) Karaca, T.; Bayiroglu, F.; Yoruk, M.; Kaya, M. S.; Uslu, S.; Comba, B.; Mis, L.
This study investigated the effects of royal jelly (RI) on acetic acid-induced colitis in rats. Twenty adult female Wistar albino rats were divided into four treatment groups of 5 animals each, including a control group (Group I); Group II was treated orally with RJ (150 mg kg(-1) body weight); Group III had acetic acid-induced colitis; and Group IV had acetic acid-induced colitis treated orally with RJ (150 mg kg(-1) body weight) for 4 weeks. Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 mL kg(-1)). Colon samples were obtained under deep anaesthesia from animals in all groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with Mallory's triple stain and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for Mast Cells). RI was shown to protect the colonic mucosa against the injurious effect of acetic acid. Colitis (colonic damage) was confirmed histomorphometrically as significant increases in the number of mast cells (MC) and colonic erosions in rats with acetic acid-induced colitis. The RI treatment significantly decreased the number of MC and reduced the area of colonic erosion in the colon of RJ-treated rats compared with rats with untreated colitis. The results suggest that oral treatment with RI could be used to treat colitis.
The Effects of Immunization Against Lhrh Using Recombinant Lhrh Fusion Protein Ol on Testicular Development, Ultrasonographic and Histological Appearance of the Testis in Buck Kids
(Amer Soc Animal Science, 2006) Ulker, H.; Kucuk, M.; Yilmaz, A.; Yoruk, M.; Arslan, L.; deAvila, D. M.; Reeves, J. J.
Effects of Photoperiod on Number of Mast Cells in Lymphoid Organs of the Japanese Quail (Coturnix Coturnix Japonica)
(2010) Karaca, T.; Ari, H.H.; Yoruk, M.; Cinaroglu, S.
The distribution of Mast Cells (MCs) was studied in the lymphoid organs of 7, 14, 21 and 30 days old quails (Coturnix coturnix Japonica) kept in different photoperiods using light microscopy histochemical techniques. The distribution of MCs was determined in different age groups housed in continuous light (23L: ID) or in a light-dark regimen (18L: 6D). Tissue samples were obtained under deep anesthesia from birds in the four age groups, fixed in Mota's fixative (basic lead acetate) for 24 h and embedded in paraffin. Six micrometre-thick sections were stained with 0.5% toluidine blue and the number of MCs counted under the microscope. The numbers of MCs were significantly different between both age and light treatment groups. The number of mast cells was significantly lower (p<0.05) in birds exposed to continuous light (23L: ID) than in birds exposed to a shorter light period (18L: 6D). Photoperiod was concluded to influence mast cell numbers in the lymphoid organs of the Japanese quail and thus the immune response of the birds. © Medwell Journals, 2010.
Endocrine and Cytological Responses To Oral Administration of Altrenogest at the Onset of the Breeding Season in Coloured Mohair Goats
(Wiley-blackwell, 2014) Uslu, S.; Belhan, S.; Uslu, B. A.; Cetin, N.; Yoruk, M.; Gulyuz, F.; Wehrend, A.
Investigation of Desensitization Effect of Deslorelin Implant (4.7 Mg) in Young Male Rats
(Wiley-blackwell, 2014) Eski, F.; Uslu, S.; Cetin, N.; Sendag, S.; Uslu, B. A.; Yoruk, M.; Wehrend, A.
Lhrh Fusion Protein Immunization Alters Testicular Development, Ultrasonographic and Histological Appearance of Ram Testis
(Wiley-blackwell Publishing, inc, 2009) Ulker, H.; Yilmaz, A.; Karakus, F.; Yoruk, M.; Budag, C.; deAvila, D. M.; Reeves, J. J.
This study was designed to evaluate the effectiveness of a luteinizing hormone releasing hormone (LHRH) fusion protein immunization on reproductive traits in ram lambs including the changes in histology and ultrasonographic appearance of testis. Thirty native ram lambs at 19 weeks of age were divided into control (C, n = 10), immunization (I, n = 10) and castration (E, n = 10) groups. Animals in immunization group were immunized against LHRH using ovalbumin-LHRH-7 (OL) protein generated by recombinant DNA technology as a primary and a booster injection at 19 and 23 weeks of age respectively. Animals were bled via jugular venepuncture at 2-week intervals to determine LHRH antibody and testosterone concentrations. Bi-weekly ultrasonographic examination of the testes was performed to determine the changes in ultrasonographic appearance as the age increased. Biopsied testicular tissues taken at 19, 29 and 41 weeks age were also evaluated. At 41 weeks of age, animals were slaughtered. Semen and epididymis were evaluated for the presence of sperm cells. Testicular development and sperm production were suppressed in the immunized animals. Semineferous tubule diameters decreased, basal membrane of the tubule was thickened and hyalinized in immunized ram lambs. While testes of control animals gained their normal ultrasonographic appearance as the age increased, immunized animals had uniform hypoechogenic testicular structure as observed at 19 weeks of age until slaughter. Simultaneous histological and ultrasonographic evaluations indicated that the changes in testicular histology could partly be monitored via ultrasonographic imaging. Nevertheless, it is difficult to claim that ultrasonographic image reflects the exact changes in such instances. In conclusion, these results indicate that recombinant OL fusion protein is effective in immunocastration in ram lambs and has a potential to be used as an alternative to physical castration. Further research studies should be conducted to help assess reproductive status of testes from ultrasound images.