Browsing by Author "Yunusoglu, Oruc"

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Adsorptive Stripping Voltammetric Determination of Higenamine on a Boron-Doped Diamond Electrode Improved by the Use of an Anionic Surfactant
(Elsevier Science Sa, 2020) Allahverdiyeva, Shabnam; Pinar, Pinar Talay; Keskin, Ertugrul; Yunusoglu, Oruc; Yardim, Yavuz; Senturk, Zuhre
The present work describes the first electroanalytical investigation and a new voltammetric method for a cheap, rapid and simple determination of higenamine at a surface of mildly-oxidized boron-doped diamond electrode (at +1.6 V during 180 s). The oxidation of compound was strongly dependent on pH. The sensitivity of method was significantly improved in the presence of anionic surfactant, sodium dodecyl sulfate (SDS). The first oxidation peak was chosen for the electroanalytical determination of higenamine due to its higher sensitivity compared to the other two ones. By employing square-wave stripping mode, there was a linear dependence of the oxidation peak current at +0.75 V and higenamine concentration in the range of 0.5-30.0 mg L-1 (1.84 x 10(-6)-1.11 x 10(-4) M), with a detection limit of 0.13 mg L-1 (4.79 x 10(-7) M) in BR buffer pH 2.0 containing 2 x 10(-4) M SDS (after 30 s accumulation at open-circuit condition). As an example, the practical applicability of the proposed method was tested with the quantification of higenamine in the commercial dietary supplement formulations, with results in good agreement to those obtained declared by high-performance liquid chromatography with diode-array detection as a reference method.
Effects of Artemisinin on Anti-Epileptogenic, Antioxidant and Cholinesterase Enzymes in Pentylenetetrazole-Induced Kindling Model in Mice
(Assoc Pharmaceutical Teachers india, 2023) Kocak, Yilmaz; Yunusoglu, Oruc; Huyut, Zubeyir; Turkan, Fikret
Background: Artemisinin (ART) is a compound synthesized from the plant Artemisia annua. This compound has various therapeutic effects and is widely used against malaria. However, ART is known to have modulating effects on GABA (gamma-aminobutyric acid) receptors, which are thought to be responsible for epileptic seizures. This study aimed to evaluate the effects of ART on anti-convulsant, antioxidant, and cholinesterase enzyme activities in Pentylenetetrazole (PTZ)-induced kindling model in mice. Materials and Methods: In the experiment, 6 groups were formed, with seven mice in each group. Mice received a total of 11 intraperitoneal injections of PTZ (35 mg/kg). On the last day of the study, a threat dose of PTZ (75 mg/kg) was administered. In addition, behavioral analysis tests (Locomotor activity and rotarod) and biochemical measurements were performed. Results: Compared with the PTZ group, ART attenuated the severity of the kindling, decreasing the seizure score. ART and VPA reversed increased oxidative stress. Decreased cholinesterase enzymes in PTZ-induced brain increased with ART treatment. While the PTZ application impaired locomotor activity in mice, the ART application provided improvement in locomotor activity. However, no significant difference was found between the groups in the motor performance of the mice. Conclusion:The findings show that ART may have the potential to prevent PTZ-induced oxidative stress, neurochemical changes, behavioral disorders, and seizures.
Effects of Chronic Ghrelin Administration on Il-1, Il-6 and Tnf- Levels in Ptz Kindling Epilepsy Model in Rats
(Karger, 2021) Erkec, Ozlem Ergul; Huyut, Zubeyir; Yunusoglu, Oruc; Kara, Busra
Effects of Fisetin on Ethanol-Induced Rewarding Properties in Mice
(Taylor & Francis inc, 2024) Yalniz, Yasin; Yunusoglu, Oruc; Berkoz, Mehmet; Demirel, Mustafa Enes
Background: Alcohol use disorder (AUD) is a chronic relapsing disorder associated with compulsive drinking of alcohol. Natural flavonoid fisetin affects a variety of transmitter systems relevant to AUD, such as aminobutyric acid, N-methyl-D-aspartate, and dopamine, as well as peroxisome proliferator-activated receptors.Objectives: This study investigated fisetin's impact on the motivational properties of ethanol using conditioned place preference (CPP) in mice (n = 50).Methods: Mice were conditioned with ethanol (2 g/kg, i.p.) or saline on alternating days for 8 consecutive days and were given intragastric (i.g.) fisetin (10, 20, or 30 mg/kg, i.g.), 45 min before ethanol conditioning. During extinction, physiological saline was injected to the control and ethanol groups, and fisetin was administered to the fisetin groups. To evaluate the effect of fisetin on the reinstatement of ethanol-induced CPP, fisetin was given 45 min before a priming dose of ethanol (0.4 g/kg, i.p.; reinstatement test day).Results: Fisetin decreased the acquisition of ethanol-induced CPP (30 mg/kg, p < .05) and accelerated extinction (20 and 30 mg/kg, p < .05). Furthermore, fisetin attenuated reinstatement of ethanol-induced CPP (30 mg/kg, p < .05).Conclusions: Fisetin appears to diminish the rewarding properties of ethanol, as indicated by its inhibitory effect and facilitation of extinction in ethanol-induced CPP. These findings imply a potential therapeutic application of fisetin in preventing ethanol-seeking behavior, promoting extinction, and reducing the risk of relapse.
The Effects of Moxidectin Nicotine-Conditioned Cue on Nicotine-Seeking Behavior in Mice
(inst Advanced Science Extension, 2021) Yunusoglu, Oruc; Demirkol, Muhammed Hamdi; Berkoz, Mehmet; Sagmanligil, Vedat; Oto, Gokhan; Ozdemir, Hulya
Current pharmacotherapies for nicotine abuse are few and relatively inefficient demonstrating the need for the development of new, effective remedies. Moxidectin is used as an anti-parasitic agent in both animals and humans, it also activates GABA receptors. The objective of the present investigation was to study the effect of moxidectin on nicotine-induced conditioned place preference (CPP) in male Swiss mice. Intraperitoneal (i.p.) route was used for nicotine (0.5mg/kg) administration for a 3-day conditioning program. The influences of moxidectin on the reinforcing characteristics of nicotine were tested in mice given i.p. treatment of moxidectin (5 and 10mg/kg) 30 minutes prior to per nicotine administration. CPP was extinguished by repeated testing, through which conditioned mice were daily given two doses of moxidectin (5 and 10mg/kg, i.p.). Subsequently, the potency of moxidectin in blocking the reinstatement of CPP provoked by priming given low-dose nicotine (0.1mg/kg, i.p.) was also evaluated. Moxidectin treatment illustrated a reserve of acquisition of nicotine-induced CPP. It was reduced priming nicotine-induced reinstatement and accelerated the extinction of CPP. Relatively nicotine enhanced the locomotor, motor activity but was not statistically significant. In conclusion, the outcomes demonstrate the potential for the development of moxidectin as a new pharmacotherapy for the treatment of nicotine addiction. (C) 2021 The Authors. Published by IASE.
Electroanalytical Investigation and Determination of Hepatitis C Antiviral Drug Ledipasvir at a Non-Modified Boron-Doped Diamond Electrode
(Elsevier Science Sa, 2020) Allahverdiyeva, Shabnam; Keskin, Ertugrul; Pinar, Pinar Talay; Yunusoglu, Oruc; Yardim, Yavuz; Senturk, Zuhre
This study is about the use of the boron-doped diamond electrode pretreated electrochemically (anodic and subsequent cathodic) in the voltammetric determination of hepatitis C antiviral drug ledipasvir. Cyclic voltammetric measurements revealed that the compound yielded two well-separated irreversible oxidation peaks (P-A1 and P-A2) in acidic medium. The dependence of intensities of currents and potentials on electrode pretreatment, pH and nature of the supporting electrolyte, and other variables was investigated by square-wave voltammetry. Determination of ledipasvir was performed in Britton-Robinson buffer (pH 2.0) on the basis of both P-A1 and P-A2, which offered linear concentration ranges 0.5-60.0 mu g mL(-1) (5.6 x 10(-7)-6.8 x 10(-5) mol L-1) and 5.0-60.0 mu g mL(-1) (5.6 x 10(-6)-6.8 x 10(-5) mol L-1), respectively, with detection limits of 0.12 mu g mL(-1) (1.4 x 10(-7) mol L-1) and 0.76 mu g mL(-1) (8.6 x 10(-7) mol L-1). The practical applicability of developed methodology was tested by the assay of a fixed-dose combination tablet with sofosbuvir.
Evaluation of Repeated Ghrelin Administration on Seizures, Oxidative Stress and Neurochemical Parameters in Pentyleneterazole Induced Kindling in Rats
(Taylor & Francis Ltd, 2024) Ergul Erkec, Ozlem; Yunusoglu, Oruc; Huyut, Zubeyir
Introduction: Epileptic seizures are thought to be caused by the impaired balance between excitatory (glutamate) and inhibitor [gamma amino butyric acid (GABA)] neurotransmitters in the brain. Neuropeptides have potent modulator properties on these neurotransmitters.Objective: Ghrelin exerts anticonvulsant effects in an acute pentylenetetrazole (PTZ) model. However, the effect of repeated ghrelin injections in chronic pentylenetetrazole kindling model is not known. In this study, the effects of repeated ghrelin administration on seizure scores, working memory, locomotor activity, oxidative biomarkers, and neurochemical parameters in PTZ kindling in rats was examined.Methods: For this purpose, 35 mg/kg of PTZ was administered intraperitoneally to the experimental groups. The rats also received physiological saline/diazepam or ghrelin before each PTZ injection. After behavioural analysis (Y-maze, rotarod, and locomotor activity tests), biochemical and neurochemical analyses were conducted using ELISA.Results: PTZ administration induced progression in the seizure scores and all of the rats in the PS + PTZ group were kindled with the 20(th) injection. Ghrelin treatment significantly reduced the seizure scores. The difference among the groups in terms of the Y-maze, locomotor activity, and rotarod tests was nonsignificant. PTZ administration significantly decreased the brain GABA, CAT, and AChE levels, and increased the MDA, NO, and protein carbonyl levels. Repeated ghrelin treatment ameliorated the GABA, AChE, CAT, MDA, NO, and protein carbonyl levels.Conclusion: Taken together, the results indicated that repeated ghrelin treatment had antioxidant, and anticonvulsant activity on PTZ kindling in rats.
The First Electroanalytical Study of Umifenovir (Arbidol) Used as a Potential Antiviral Drug for The Treatment of Sars-Cov a Voltammetric Quantification on The Boron-Doped Diamond Electrode by Using Anionic Surfactant Media
(Electrochemical Soc inc, 2023) Ozok, Hande Izem; Kiran, Musa; Yunusoglu, Oruc; Yardim, Yavuz
In this work, an electroanalytical procedure for sensing umifenovir (arbidol) by square wave adsorptive stripping voltammetry (SW-AdSV) was developed utilizing an anodically pretreated boron-doped diamond electrode. Measurements of umifenovir using cyclic voltammetry with phosphate buffer solution (PBS, 0.1 M, pH 2.5) revealed irreversible behaviour, adsorption-controlled as well as an ill-defined (+1.13 V, P-A1) and a well-defined (+1.47 V, P-A2) two oxidation peaks. Umifenovir oxidations depend critically on supporting electrolytes and pH. The second oxidation peak (P-A2) current of the umifenovir was enhanced by adding sodium dodecyl sulfate (SDS, anionic surfactant) in the chosen supporting electrolyte. Umifenovir was quantified using its second oxidation peak (P-A2) at about +1.39 V. Using the optimized condition, the oxidation peak current of P-A2 showed a linear relationship for umifenovir determination in the concentration range from 0.005 to 1.0 mu g ml(-1) (9.73 x 10(-9)-1.95 x 10(-6) M), with a detection limit of 0.0014 mu g ml(-1) (2.72 x 10(-9) M) in PBS (PH 2.5) with SDS. Finally, the developed approach was successfully utilized to determine umifenovir in the pharmaceutical formulation and urine samples. To the best of our knowledge, this is the first electroanalytical approach for voltammetric sensing of umifenovir.
First Electrochemical Evaluation of Favipiravir Used as an Antiviral Option in the Treatment of Covid-19: a Study of Its Enhanced Voltammetric Determination in Cationic Surfactant Media Using a Boron-Doped Diamond Electrode
(Elsevier, 2021) Allahverdiyeva, Shabnam; Yunusoglu, Oruc; Yardim, Yavuz; Senturk, Zuhre
Favipiravir, a promising antiviral agent, is undergoing clinical trials for the potential treatment of the novel coronavirus disease 2019 (COVID-19). This is the first report for the electrochemical activity of favipiravir and its electroanalytical sensing. For this purpose, the effect of cationic surfactant, CTAB was demonstrated on the enhanced accumulation of favipiravir at the surface of cathodically pretreated boron-doped diamond (CPT-BDD) electrode. At first, the electrochemical properties of favipiravir were investigated in the surfactant-free solutions by the means of cyclic voltammetry. The compound presented a single oxidation step which is irreversible and adsorption controlled. A systematic study of various operational conditions, such as electrode pretreatment, pH of the supporting electrolyte, concentration of CTAB, accumulation variables, and instrumental parameters on the adsorptive stripping response, was examined using square-wave voltammetry. An oxidation signal at around +1.21 V in Britton-Robinson buffer at pH 8.0 containing 6 x 10(-4) M CTAB allowed to the adsorptive stripping voltammetric determination of favipiravir (after 60 s accumulation step at open-circuit condition). The process could be used in the concentration range with two linear segments of 0.01-0.1 mg mL(-1) (6.4 x 10(-8-)6.4 x 10(-7) M) and 0.1-20.0 mg mL(-1) (6.4 x 10(-7)-1.3 x 10(-4) M). The limit of detection values were found to be 0.0028 mg mL(-1) (1.8 x 10(-8) M), and 0.023 mg mL(-1) (1.5 x 10(-7) M) for the first and second segments of calibration graph, respectively. The feasibility of developed methodology was tested to the analysis of the commercial tablet formulations and model human urine samples. (C) 2021 Elsevier B.V. All rights reserved.
Hepatoprotective Potentials of Usnea Longissima Ach. and Xanthoparmelia Somloensis (Gyelnik) Hale Extracts in Ethanol-Induced Liver Injury
(Taylor & Francis Ltd, 2025) Berkoz, Mehmet; Aslan, Ali; Yunusoglu, Oruc; Krosniak, Miroslaw; Francik, Renata
In our study, the antioxidant and anti-inflammatory effects of different lichen applications were investigated in rats using an experimental ethanol toxicity model. 48 rats were used in the study and they were divided into 6 groups with 8 rats in each group. These groups were: control, ethanol (2 g/kg), ethanol + Usnea longissima Ach. (200 mg/kg), ethanol + Usnea longissima Ach. (400 mg/kg), ethanol + Xanthoparmelia somloensis (Gyelnik) Hale (100 mg/kg) and ethanol + Xanthoparmelia somloensis (Gyelnik) Hale (200 mg/kg). The experimental work continued for 21 days. Lichen extracts and ethanol were administered by gavage to rats divided into groups. According to the experimental protocol, the experimental animals were sacrificed and their liver tissues were isolated. Biochemical parameters in serum, histological examinations, oxidative stress and inflammation parameters both at biochemical and molecular level in liver tissues were performed. Oxidative stress and inflammatory response were increased in the liver tissue of rats treated with ethanol for 21 days, and liver functions were impaired. It was found that U. longissima and X. somloensis extracts showed good antioxidant activity and conferred protective effects against ethanol-induced oxidative stress and inflammation. This could be attributed to the presence of secondary metabolites in the extract, which act as natural antioxidants and could be responsible for increasing the defence mechanisms against free radical production induced by ethanol administration.
Investigation of Antiepileptic Potentials of Usnic Acid and Some Lichen Species on the Behavioral and Biochemical Levels in Pentylenetetrazole-Induced Kindling Model of Epilepsy
(Marmara Univ, 2024) Berkoz, Mehmet; Yunusoglu, Oruc; Aslan, Ali; Bozkurt, Ayse
In this study, the effects of various lichen and usnic acid applications on seizure scores and biochemical parameters in brain tissue in rats with epilepsy model was investigated. For this aim, 91 rats were divided into 13 groups, each containing 7 rats, which were: control, pentylenetetrazole (PTZ) (35 mg/kg), PTZ + Valproic acid (100 mg/kg), PTZ + Dolichousnea longissima(200 mg/kg), PTZ + Dolichousnea longissima (400 mg/kg), PTZ + Xanthoparmelia somloensis( 50 mg/kg), PTZ + Xanthoparmelia somloensis(200 mg/kg), PTZ + Cetraria islandica(250 mg/kg), PTZ + Cetraria islandica(500 mg/kg), PTZ + Pseudevernia furfuracea(250 mg/kg), PTZ + Pseudevernia furfuracea(500 mg/kg), PTZ + usnic acid (50 mg/kg), and PTZ + usnic acid (200 mg/kg). All items were applied with an interval of 120 minutes for a period of one week. Seizure detection, seizure scores and total seizure duration of each group was recorded. After the applications, oxidative stress parameters and acetylcholinesterase enzyme activity in the brain tissue of rats were measured. There was no difference between the groups in the 1st, 2nd, and 3rdinjections (p>0.05). Starting from the 4th injection, the seizure score was significantly higher in the PTZ group compared to the control group (p<0.05). When the effects on locomotor activity were evaluated, no difference was found between any group (p>0.05). In PTZ applied groups, an increase in lipid and protein oxidation as well as a decrease in antioxidant and acetylcholine esterase levels were observed(p<0.05). Valproic acid, high concentration of lichen extract applications and high and low concentration of usnic acid applications were found to reverse this situation (p<0.05). As a result, various lichen extracts and usnic acid were shown to reduce behavioral symptoms and oxidative stress of epilepsy, with a preventive effect on the complications of epilepsy.
Investigation of the Impact of Antiparasitic Drug Moxidectin on the Rewarding Effects of Alcohol
(Aepress Sro, 2022) Ekici, Abdurrahman; Gurbuz, Esra; Berkoz, Mehmet; Turkmen, Omer; Basbugan, Yildiray; Yunusoglu, Oruc
Alcohol addiction or alcoholism constitutes a significant risk factor worldwide for morbidity and mortality. Moxidectin is a recently approved anthelmintic drug, which also activates the gamma-aminobutyric acid receptors. The objective of the present study was to examine the impact of moxidectin on rewarding effects of ethanol in the conditioned place preference (CPP) model in mice. In separate experiments, mice were administered intraperitoneal (i.p.) injections of moxidectin (5 or 10 mg/kg) before a) acquisition of alcohol-induced CPP, b) each extinction session, and c) alcohol-induced reinstatement of CPP. The present experiments provide consistent data about ethanol place preference in mice (2 g/kg, i.p.), with mice in all tests spending significantly more time on the ethanol-paired side. The acquisition of the CPP response to ethanol was prevented by the administration of moxidectin at a dose of 10 mg/kg. Additionally, moxidectin treatment accelerated the extinction of ethanol CPP when given repeatedly during the extinction phase. Ethanol-induced reinstatement of CPP following an extinction phase was inhibited by moxidectin. Ethanol alone and co-administration with moxidectin did not change locomotor activity and motor coordination. In conclusion, we suggest that moxidectin may be a promising therapeutic candidate for prevention of ethanol-induced addiction and relapse as well as detoxification.
Investigation of the Pharmacological Potential of Myricetin on Alcohol Addiction in Mice
(Marmara Univ, 2022) Yunusoglu, Oruc; Shahzadi, Andleeb; Turel, Canan Akunal; Demirkol, Muhammed Hamdi; Berkoz, Mehmet; Akkan, Ahmet Gokhan
Alcohol addiction is one of the leading causes which is associated with morbidity and mortality with outcomes in high healthcare and economic costs. Myricetin is a flavonoid that demonstrates therapeutic actions in many central nervous system diseases. In the current study, the conditioned place preference (CPP) tests were performed W examine the effects of myricetin on ethanol reward. During conditioning, intraperitoneal (i.p) administration of ethanol (2 g/kg) and serum physiologic were given on alternate days for 8 days. In order to evaluate the effect of myricetin on the development of alcohol addiction, myricetin was injected into mice 30 minutes before ethanol administration. Subsequently, a daily myricetin injection was performed to evaluate the effect of myricetin on the extinction of alcohol addiction. Finally, ethanol was administered 900 seconds after different dose myricetin administration, and reinstatement was evaluated immediately thereafter. Systemic ethanol (2 g/kg, i.p) administration significantly produced CPP. Myricetin (5 and 10 mg/kg, i.p) attenuated the development of ethanol addiction (p < 0.05). Systemic myricetin injections immediately after each extinction period precipitated extinction and decreased reinstatement (10 mg/kg, i.p, p < 0.05, respectively). Ethanol alone and in combination with myricetin did not change locomotor activity and motor coordination. As a result, it can be suggested that myricetin is effective in attenuating the rewarding effect of alcohol in mice and can be used for the adjunctive therapy for alcohol addiction. In addition, it will be appropriate to conduct mechanistic experimental studies regarding these results in the future.
Investigation of the Pharmacological, Behavioral, and Biochemical Effects of Boron in Parkinson-Indicated Rats
(C M B Assoc, 2022) Ozdemir, Hulya Sagmanligil; Yunusoglu, Oruc; Sagmanligil, Vedat; Yasar, Semih; Colcimen, Nese; Goceroglu, Rezzan Temelli; Catalkaya, Ege
Parkinson's disease (PD) is a progressive neurodegenerative disorder of the central nervous system. In different studies, it has been investigated that boric acid has positive effects on different mechanisms that are important in PD. The aim of our study was to investigate the pharmacological, behavioral and biochemical effects of boric acid on rats with experimental PD with Rotenone. For this purpose, Wistar-albino rats were divided into 6 groups. Only normal saline was applied subcutaneously (s.c) to the first control and sunflower oil to the second control group. Rotenone was administered (s.c) to 4 groups (groups 3-6) at a dose of 2 mg/kg for 21 days. Only rotenone (2mg/kg, s.c) was administered to the third group. Boric acid was administered intraperitoneally (i.p.) at 5 mg/kg, 10 mg/kg, and 20 mg/kg to groups 4, 5, and 6, respectively. During the study, behavioral tests were applied to the rats, and then histopathological and biochemical analyzes were performed from the sacrificed tissues. According to the data obtained, a statistically significant difference (p<0.05) was observed between the Parkinson's group and the other groups in motor behavior tests, excluding the catalepsy test. Boric acid exhibited dose-dependent antioxidant activity. As a result of the histopathological and immunohistochemical (IHC) examination, a decrease in neuronal degeneration was observed at the increasing doses of boric acid, while gliosis and focal encephalomalacia were rarely encountered. There was a significant increase in tyrosine hydroxylase (TH) immunoreactivity, especially in group 6, with a dose of 20 mg/kg of boric acid. From these results, we conclude that the dose-dependent effect of boric acid may protect the dopaminergic system with antioxidant activity in the pathogenesis of PD. However, the effectiveness of boric acid on PD needs further investigation in a larger, more detailed study using different methods.
Linalool Attenuates Acquisition and Reinstatement and Accelerates the Extinction of Nicotine-Induced Conditioned Place Preference in Male Mice
(Taylor & Francis inc, 2021) Yunusoglu, Oruc
Background: Nicotine is the addictive agent in tobacco products. The monoterpene linalool is the main ingredient in the essential oils of various aromatic plants. It has previously been demonstrated that linalool has beneficial effects on some mechanisms that are important in drug addiction. Objectives: The goal of the current study was to investigate the effect of linalool on nicotine-induced conditioned place preference (CPP) in male mice. Methods: CPP was induced by administering intraperitoneal (i.p.) injection of nicotine (0.5 mg/kg) during the conditioning phase. The effects of nicotinic acetylcholine receptor partial agonist varenicline and linalool on the rewarding characteristics of nicotine were tested in mice with administration of linalool (12.5, 25, and 50 mg/kg, i.p.), varenicline (2 mg/kg, i.p.) or saline 30 minutes before nicotine injection. CPP was extinguished by repeated testing, during which conditioned mice were administered varenicline and linalool every day. One day after the last extinction trial, mice that received linalool, varenicline or saline 30 minutes before a priming injection of nicotine (0.1 mg/kg, i.p.) were immediately tested for reinstatement of CPP. Results: Linalool attenuated nicotine acquisition (50 mg/kg, p < .01) and reinstatement (25 and 50 mg/kg, respectively p < .05, p < .01) and accelerated the extinction of nicotine-induced CPP (50 mg/kg, p < .05). Linalool exhibited similar effects on the reference drug varenicline in the CPP phases. Conclusion: These results suggest that linalool may be helpful as an adjuvant for the treatment of nicotine use disorder.
Myricetin Inhibits Angiotensin Converting Enzyme and Induces Nitric Oxide Production in Huvec Cell Line
(General Physiol and Biophysics, 2020) Berkoz, Mehmet; Yildirim, Metin; Yalin, Serap; Ilhan, Mert; Yunusoglu, Oruc
Nitric oxide is known as relaxing factor because it acts as a vasodilator, increases blood flow, and inhibits platelet aggregation and adhesion, on the other hand nitric oxide can modulate cellular and physiological processes to limit oxidative injury, limiting processes such as leukocyte adhesion. As the complete mechanism of myricetin and its cardiovascular benefits is not completely understood, the aim of this study was to investigate the antihypertensive activity of myricetin in human umbilical vein endothelial cell (HUVEC). Angiotensin converting enzyme (ACE) activity, nitric oxide production, reactive oxygen species (ROS) scavenger activity, cellular calcium concentration, and endothelial nitric oxide synthase (eNOS) activity and protein expression was investigated in HUVEC treated with different concentration of myricetin (1-60 mu M). Myricetin increased nitric oxide production in HUVEC through decreased ROS levels and increased nitric oxide production and eNOS activation. Activation of eNOS enzyme was achieved by an increase of cellular calcium concentration. At the same examined concentration of myricetin, the activity of ACE was significantly inhibited. These findings indicate that myricetin may be helpful for lowering blood pressure; this could be achieved through dietary intervention or by the production of new antihypertensive treatments from a natural product.
Prophylactic Effect of Myricetin and Apigenin Against Lipopolysaccharide-Induced Acute Liver Injury
(Springer, 2021) Berkoz, Mehmet; Unal, Seda; Karayakar, Fahri; Yunusoglu, Oruc; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Aslan, Ali
Background Liver has an important role in the initiation and progression of multiple organ failure that occurs in sepsis. Many natural active substances can be used to reduce the liver injury caused by sepsis. For this aim, the effects of myricetin and apigenin on mice model of acute liver injury was evaluated in this study. Methods and results Thirty-six mice were randomly divided into six groups as; control, lipopolysaccharide (LPS) (5 mg/kg), LPS + myricetin (100 mg/kg), LPS + myricetin (200 mg/kg), LPS + apigenin (100 mg/kg), and LPS + apigenin (200 mg/kg) groups. Myricetin and apigenin were administered orally for 7 days, and LPS was administered intraperitoneally only on the 7th day of the study. 24 h after LPS application, all animals were sacrificed and serum biochemical parameters, histopathology and oxidative stress and inflammation markers of liver tissue were examined. Myricetin and apigenin pre-treatments increased serum albumin and total protein levels, liver GSH level and catalase and SOD activities and decreased serum ALT, AST, ALP, gamma-GT, CRP, total and direct bilirubin levels, liver MPO activity, MDA, NOx, PGE(2), TNF-alpha, IL-1 beta, and IL-6 levels, iNOS and COX-2 mRNA levels, phosphorylation of NF-kappa B p65, I kappa B, and IKK proteins but not p38, ERK, and JNK proteins in LPS-treated mice. Myricetin and apigenin administration also regained the hepatic architecture disrupted during LPS application. Conclusion Myricetin and apigenin pre-treatments led to reduction of liver injury indices and oxidative stress and inflammatory events and these flavonoids has probably hepatoprotective effects in acute liver injury.
Resveratrol Impairs Acquisition, Reinstatement and Precipitates Extinction of Alcohol-Induced Place Preference in Mice
(Taylor & Francis Ltd, 2021) Yunusoglu, Oruc
Objective Alcohol abuse causes several neurological disorders. Resveratrol is a natural polyphenol that occurs as a phytoalexin. In different studies, it has been investigated that resveratrol has positive effects on various mechanisms that are important in drug addiction or substance use disorder. The objective of the present study was to examine the effect of resveratrol on alcohol-induced conditioned place preference (CPP) in mice. Methods CPP was induced by intraperitoneal (i.p.) administration of ethanol (2 g/kg) in an 8-day conditioning program. The influence of reference drug, acamprosate and resveratrol on the rewarding properties of ethanol was tested in mice given treatment of acamprosate (300 mg/kg, i.p.) and resveratrol (25, 50, and 75 mg/kg, i.p.) 30 minutes prior to ethanol administration. Once established, CPP was extinguished by repeated testing, through which conditioned mice were administered acamprosate, various doses of resveratrol or saline daily. Subsequently, the potency of acamprosate and resveratrol in preventing reinstatement of CPP provoked by priming with low-dose ethanol (0.4 g/kg, i.p.) was also evaluated. Results The present findings confirm that resveratrol impairs acquisition, reinstatement and precipitates the extinction of preference for alcohol-induced CPP. Resveratrol presented a similar effect in the CPP phases to the acamprosate. Conclusions The effect of resveratrol on ethanol-induced CPP in mice demonstrated for the first time. As a conclusion, these findings may shed light on the fact that resveratrol can be utilized as an agent which is potentially beneficial to prevent the various harmful effects of ethanol, however, more research is needed to completely elucidate this attribute.
Roe Protein Hydrolysate of Alburnus Tarichi Induces Apoptosis in Breast Cancer Mcf-7 and Mda-Mb Cells Through a Caspase-Dependent Pathway
(General Physiol and Biophysics, 2020) Berkoz, Mehmet; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Krosniak, Miroslaw; Turkmen, Omer; Yunusoglu, Oruc
The protein hydrolysates of fishes have been reported to be a potential source of many health benefits components for pharmaceutical or nutritional applications. The aim of this study is to examine the possible antiproliferative function of roe protein hydrolysates of Alburnus tarichi using enzymatic hydrolysis against breast cancer cells and explore its detailed mechanisms. In addition, we evaluated the effects of protein hydrolysate on the proliferation and apoptosis of two human breast cancer cell lines (MCF-7 and MDA-MB-231). The cultured cells were treated with protein hydrolysate at concentrations of 0-5 mu g/ml for 24 h and 48 h. Inhibition of cell proliferation, percentage of apoptotic cells, cell cycle distribution, morphological changes, DNA fragmentation, intracellular reactive oxygen species (ROS) production, and apoptotic protein levels were also examined. Decreases in proliferation of MCF-7 and MDA-MB-231 cells were observed after treatment with the protein hydrolysate in a dose-dependent manner. Distinct morphological changes, a typical pattern of fragmented DNA, and increased intracellular ROS production and apoptotic protein levels were observed in both cell lines after hydrolysate treatment (p < 0.05). The results suggested that the protein hydrolysate inhibits the proliferation of human breast cancer cell lines by introducing apoptosis through a caspase-dependent pathway in a dose-dependent manner.
Sensing Ivacaftor Accomplished Using the Square-Wave Voltammetric Technique With the Assistance of a Cationic Surfactant on a Boron-Doped Diamond Electrode
(Elsevier Science Sa, 2024) Barzani, Hemn A. H.; Ali, Hoshyar Saadi; Yunusoglu, Oruc; Yardim, Yavuz
This investigation aims to describe the voltammetric analysis of ivacaftor (IVA) by utilizing the boron -doped diamond (BDD) electrode to add a cationic surfactant. Cyclic voltammetry via Britton-Robinson (BR, 0.04 mol L-1, pH 2.0) buffer was used to perform determinations of the IVA demonstrating irreversible behaviors, adsorption-controlled and well-defined (+1.04 V, PA1) and an ill-defined (+1.42 V, PA2) oxidation peaks (vs. Ag/ AgCl). The findings revealed that the oxidation peaks of IVA are pH-dependent (ranging from 2.0 to 5.0). The use of cetyltrimethylammonium bromide (CTAB, cationic surfactant) in the chosen supporting electrolyte enormously raised the oxidation peak currents of IVA. For the measurement of IVA in a 0.04 mol L-1 BR buffer solution with a pH of 2.0, the linear relationship was discovered to exist under the conditions of the experimental optimal parameters involving 2 x 10-4 mol L-1 CTAB at +1.11 V (vs. Ag/AgCl) (after an accumulation of 60 s at the open-circuit condition). The linear concentration was discovered using 0.25 to 10.0 mu g mL-1 (6.4 x 10-7-2.5 x 10-5 mol L-1) and the limit of detection 0.073 mu g mL-1 (1.9 x 10-7 mol L-1). The devised methodology was effectively employed to determine IVA in pharmaceutical formulation. To the best of our understanding, it represents the first electroanalytical method for detecting IVA by voltammetry.