TR-Dizin İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14720/5

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First Report of ‘candidatus Phytoplasma Australasia’ Strain Related To Witches’-Broom of Tomato in Türkiye
(Centenary University, 2023) Usta, M.; Güller, A.; Sipahioğlu, H.M.
Phytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, the identification of phytoplasmas infecting tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Five plants, two symptomatic and three asymptomatic, were tested to verify phytoplasma infection. Total DNA isolated from 5 leaf samples was used as a template for PCR reactions. The phytoplasma agents were confirmed in the two symptomatic samples. BLASTn search of 16S rRNA of two sequences shared identity similarity of 99.84% with ‘Candidatus Phytoplasma australasia’. Computer-simulated virtual RFLP profiles show that the 16S rRNA sequences is identical to the reference pattern of the 16SrII-D subgroup, with a similarity coefficient of 1.00. Based on BLAST, virtual RFLP, and phylogenetic dendrogram, the identified phytoplasma strains are enclosed in the 16SrII-D subgroup. This is the first report of tomato witches' broom disease related to 16SrII-D subgroup phytoplasma strains in the Antalya province of Türkiye. © 2023, Centenary University. All rights reserved.
Identification and Antimicrobial Activity of Actinobacteria Isolated From Rhizosphere of the Local Endemic Plants
(Centenary University, 2021) Özakin, S.; Taşkin, B.; İnce Bostanci, E.
The search for novel antimicrobial compounds from the environment has been gaining momentum with the increase in resistance of pathogens. In the present study, the antimicrobial potential of 11 local isolates of Actinobacteria which were isolated from rhizosphere soils of the three local endemic plants in Turkey was evaluated against different pathogenic bacteria and Candida albicans. Antimicrobial activity was investigated by the disk diffusion agar method using the organic extracts obtained from the isolates grown in the modified Bennett's medium and Tryptic Soy Broth (TSB). Eight isolates were revealed to show remarkable antimicrobial activity against to pathogens. Study of the production of bioactive metabolites in two different culture media indicated that the higher antimicrobial activity was observed in modified Bennett's medium when compared to TSB. Also, partial 16S rDNA sequence analyses revealed that 10 of these local isolates belong to member of Streptomyces and one of them to member Promicromonospora genera. © 2021, Centenary University. All rights reserved.

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