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TR-Dizin İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14720/5

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Browsing TR-Dizin İndeksli Yayınlar Koleksiyonu by Subject "18S Rrna"

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    Article
    Molecular Identification Using 18s Ribosomal Rna of Sarcocystis Spp. in Bovine Minced Meat in Van Province, Turkey
    (Ankara Univ Press, 2021) Oguz, Bekir; Deger, M. Serdar; Kosal, Sena
    This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey. For this purpose a total of 150 samples were obtained from Ipekyolu, Tusba, Edremit, Ercis, and Gevas districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods. Sarcocystis cruzi (96.6%) was detected in all samples that were detected positive using molecular methods. Sarcocystis hominis-like was found in 143 (95%) samples whereas S. hirsuta was detected in only 4 (2.6%) samples. According to the Basic Local Alignment Search Tool (BLASTN) analysis of the 18S rRNA gene region of the S. cruzi (MN832695) and S. hirsuta (MN832697) isolates, they showed 100% similarity to the samples (MH681972; MH681973) that were submitted to GenBank from China. The BLASTN analysis of the 18S rRNA gene region of the S. hominis-like isolate (MN832696) revealed that it was 99.45% identical to the S. bovini (KT901155) isolated from a water buffalo in New Zealand. In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study.
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    Morphology and Phylogeny of a New Soil Ciliate, Colpodidium Zelihayildizae N. Sp. (Ciliophora, Nassophorea, Colpodidiidae), From Van, Turkey
    (Tubitak Scientific & Technological Research Council Turkey, 2021) Yildiz, Ismail
    A new ciliate species Colpodidium zelihayildizae n. sp., which was discovered in a dry irrigation channel in Van (Turkey), was investigated morphologically using standard live and silver impregnation methods, and its SSU rRNA gene phylogeny was provided. This new species was characterized as follows: body size about 40-60 x 15-20 mu m in vivo, elongated ellipsoidal with flat or slightly concave ventral surface; the buccal cavity slightly below equatorial plane; macronucleus spherical or slightly elliptical, usually located in the last third of the cell; cortex distinctly furrowed in the anterior half; 19-22 somatic kineties, 4 postoral kineties; paroral membrane has 20 dikinetids; 3 nassulid organelles (NOs), with NO1 very close to the left edge of the paroral membrane, which consists of 1 dikinetid pair. Phylogenetic analyses conducted on the SSU rDNA sequences determined that Colpodidium zelihayildizae was clustered with Colpodidium caudatum and formed a clade with Apocolpodidium etoschense and Colpodididae sp.