Antidiabetic and Antioxidant Effects of Vitis Vinifera L. Cv. 'kara Erik' Seed Extract in Streptozotocin Diabetic Rats

Abstract

In this study was aimed to investigate effects on the levels of liver and renal tissue CAT, MDA, SOD, GPx and GSH activities of Vitis vinifera L. cv. 'Kara Erik' seed extract on diabetic rats. A total of 28 male rats were randomly divided to four groups containing of 7 rats per group. Control Group: Received only vehicle 0.5% Carboxymethyl cellulose (CMC), STZ-DM (Streptozotosin-Diabetus mellitus) Group: Streptozotocin 60 mg/kg/IP single dose, VVE (Vitis vinifera L. seed extract) Group: Vitis vinifera seed extract 100 mg/kg/20days/po, STZ-DM+VVE Group: STZ as 60 mg/kg/IP single dose + VVE100 mg/kg/20days/po. At the end of these processes, the animals were sacrificed, liver and renal tissue were excised by dissection and evaluated. Catalase (CAT), Malondialdehyde (MDA), Superoxide dismutase (SOD), Glutathione peroxidase (GPx) and Glutathione (GSH) were measured in the liver and renal homogenisates. VVE was shown that the activity of CAT, SOD and GSH activities increased and decreased levels of MDA in diabetic rats. As a result, the results showed that oral administration of VVE extract reduced the levels of lIPid peroxides and improved the antioxidant activity in liver and renal tissues in rats treated with V. vinifera seed extract as compared with the diabetic and control rats. These results suggested that the V. vinifera L. cv. seed extract enhanced the antioxidant defense under hyperglycaemic conditions hence protected the liver and renal cells.