A Pcr Method With Internal Control for Detection of Brucella Spp. From Bovine Abortion Samples
| dc.authorid | Altug, Nuri/0000-0001-5805-0340 | |
| dc.authorwosid | Altug, Nuri/Aaj-3790-2020 | |
| dc.authorwosid | Solmaz, Hasan/Lze-0276-2025 | |
| dc.authorwosid | Altug, Nuri/E-3427-2018 | |
| dc.contributor.author | Solmaz, H. | |
| dc.contributor.author | Cantekin, Z. | |
| dc.contributor.author | Altug, N. | |
| dc.contributor.author | Ilhan, Z. | |
| dc.contributor.author | Aslan, S. | |
| dc.contributor.author | Ergun, Y. | |
| dc.date.accessioned | 2025-05-10T16:57:35Z | |
| dc.date.available | 2025-05-10T16:57:35Z | |
| dc.date.issued | 2014 | |
| dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
| dc.department-temp | [Solmaz, H.] Yuzuncu Yil Univ, Fac Pharm, Dept Pharmaceut Microbiol, TR-65100 Van, Turkey; [Cantekin, Z.] Mustafa Kemal Univ, Dept Microbiol, Fac Vet Med, TR-31000 Antakya, Turkey; [Altug, N.] Mustafa Kemal Univ, Fac Vet Med, Dept Internal Dis, TR-31000 Antakya, Turkey; [Ilhan, Z.] Yuzuncu Yil Univ, Fac Vet Med, Dept Microbiol, TR-65080 Van, Turkey; [Aslan, S.] Adana Vet Control & Res Inst, Adana, Turkey; [Ergun, Y.] Mustafa Kemal Univ, Fac Vet Med, Dept Obstet & Gynaecol, TR-31000 Antakya, Turkey | en_US |
| dc.description | Altug, Nuri/0000-0001-5805-0340 | en_US |
| dc.description.abstract | Brucella spp. are important infectious agents in bovine abortions worldwide. The bacteriological culture of Brucella spp. is fastidious and time consuming procedure as a classical laboratory method. Brucella spp. can be detected by using different molecular techniques. The aim of this study is to develop a PCR technique with an internal control for detection of Brucella spp. from bovine abortion samples. For this purpose, the sensitivities of three different primer pairs (BgF/BgR, B4/B5 and JP-R/JP-F) were compared. Bovine 12S gene specific primer pairs (12SM-FW/12SBT-REV2) were used as an internal control. The sensitivity of BgF/BgR primers was found higher than the other primer sets. A PCR assay was developed by combining BgF/BgR primer sets and primers for bovine 12S. This protocol was tested and validated by using abomasal contents of two Brucella-positive and eighteen Brucella-negative clinical samples. In conclusion, the developed PCR method with an internal positive control has a potential for use in direct detection and identification of the Brucella spp. from bovine abortion samples. | en_US |
| dc.description.woscitationindex | Science Citation Index Expanded | |
| dc.identifier.endpage | 257 | en_US |
| dc.identifier.issn | 0035-1555 | |
| dc.identifier.issn | 2258-0646 | |
| dc.identifier.issue | 9-10 | en_US |
| dc.identifier.scopusquality | N/A | |
| dc.identifier.startpage | 253 | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.14720/4106 | |
| dc.identifier.volume | 165 | en_US |
| dc.identifier.wos | WOS:000343280600001 | |
| dc.identifier.wosquality | N/A | |
| dc.language.iso | en | en_US |
| dc.publisher | Ecole Nationale veterinaire Toulouse | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Brucella Spp., Pcr | en_US |
| dc.subject | Internal Control | en_US |
| dc.title | A Pcr Method With Internal Control for Detection of Brucella Spp. From Bovine Abortion Samples | en_US |
| dc.type | Article | en_US |