Investigations of the Inhibition Kinetics of Some Drugs and Chemicals on Enzyme of Polyphenol Oxidase Purified From Apricot's (Salak)
| dc.authorscopusid | 23570725000 | |
| dc.authorscopusid | 56259656000 | |
| dc.authorscopusid | 14066125000 | |
| dc.authorscopusid | 10045994700 | |
| dc.contributor.author | Cimen, C. | |
| dc.contributor.author | Demir, H. | |
| dc.contributor.author | Gur, A. | |
| dc.contributor.author | Kubilay, S. | |
| dc.date.accessioned | 2025-05-10T17:51:22Z | |
| dc.date.available | 2025-05-10T17:51:22Z | |
| dc.date.issued | 2007 | |
| dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
| dc.department-temp | Cimen C., Science and Arts Faculty, Department of Chemistry, Yuzuncu Yil University, Van - 650 80, Turkey; Demir H., Science and Arts Faculty, Department of Chemistry, Yuzuncu Yil University, Van - 650 80, Turkey; Gur A., Science and Arts Faculty, Department of Chemistry, Yuzuncu Yil University, Van - 650 80, Turkey; Kubilay S., Science and Arts Faculty, Department of Chemistry, Yuzuncu Yil University, Van - 650 80, Turkey | en_US |
| dc.description.abstract | Polyphenol oxidase (PPO) was purified from Igdir apricot, with a 367 fold purification of PPO by affinity chromatography being achieved. Amount of the protein was determined according to Bradford method. Vmax and Km values were found by means of Lineweaver- Burk graphs. Asetly salisilic acid, paracetamol, ascorbic acid (vitamin C), sodium sulphate, copper sulphate, glucose, sodium nitrite, sodium chlorure, glisine, sodium azide, 2-merkaptoethanol, tyrosine, citric acid, etilendiamin tetra acetic acid (EDTA) ve p-amino benzoic acid were used as inhibitor. Inhibition constants Ki of each inhibitor were found from Lineweaver-Burk graphs. It was found that the p-aminobenzoik acid function showed the highest inhibitory effect. | en_US |
| dc.identifier.endpage | 664 | en_US |
| dc.identifier.issn | 0973-1245 | |
| dc.identifier.issue | 2 | en_US |
| dc.identifier.scopus | 2-s2.0-39649095181 | |
| dc.identifier.scopusquality | N/A | |
| dc.identifier.startpage | 659 | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.14720/18102 | |
| dc.identifier.volume | 4 | en_US |
| dc.identifier.wosquality | N/A | |
| dc.language.iso | en | en_US |
| dc.relation.ispartof | Biosciences Biotechnology Research Asia | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Characterization | en_US |
| dc.subject | Inhibitor | en_US |
| dc.subject | Kinetics | en_US |
| dc.subject | Polyphenol Oxidase | en_US |
| dc.subject | Purification | en_US |
| dc.title | Investigations of the Inhibition Kinetics of Some Drugs and Chemicals on Enzyme of Polyphenol Oxidase Purified From Apricot's (Salak) | en_US |
| dc.type | Article | en_US |