Investigation of Genetic Polymorphisms of Csn1s1 and Blg Genes in Norduz Sheep by Pcr-Rflp Method

Abstract

This study was undertaken to examine genetic polymorphisms of Alpha-Si casein (CSN1S1) and β-Lactoglobulin (BLG) genes in Norduz sheep by PCR-RFLP method. CSN1S1 and BLG genes have known a significant effect on cheese making. Restriction fragment length polymorphism (RFLP) is a molecular biology tool that reveals the difference between samples of homologous DNA molecules from differing locations of restriction enzyme sites.10 ml blood samples from 102 heads of Norduz sheep was used in the study.The genotypes of CSN1S1 and BLG genes was determination by PCR-RFLP method. Statistical analysis, after calculating allele and genotype frequencies by direct gene counting method, the distribution of observed and expected genotypic frequencies was determined according to the Hardy-Weinberg Equilibrium, and whether it was compatible with the x2 test.In the study conducted in Norduz sheep, polymorphism was observed in CSN1S1 and BLG genes. AA, AB and BB genotype frequencies of BLG gene were found to be 17.6%, 69.6% and 12.7%, respectively. It was determined that the BLG gene was not in Hardy-Weinberg Equilibrium. AA, AC and CC genotype frequencies of CSN1S1 gene were determined as 0.0%, 2.9% and 97.1%, respectively. The CSN1S1 gene was determined to be in Hardy-Weinberg equilibrium. In conclusion, it was determined that the A allele and AB genotype of the BLG gene were more common, the C allele and the CC genotype of the CSN1S1 gene were more common however, the AA genotype was not observed in Norduz sheep. © 2023 B Çak, O Yılmaz, AF Demirel. All Rights Reserved.