Collection of Semen From Van Cats Using Electroejaculation and Freezing of Semen

Abstract

In this study, the aim was to collect semen from Van Cats using electroejaculation, to perform spermatologic examinations and to freeze the semen. In the research, 7 Male Van Cats were used. Semen was collected from cats at 10 different times at weekly intervals using electroejaculation with general anesthesia. The semen was diluted with 3 diluents (3% Equiex, 6% Equiex, Biosfos). Motility and vitality values were analyzed. Then the semen was frozen in liquid nitrogen vapor. The semen dissolved was analyzed for motility and vitality values. In the study, amount of semen, sperm motility, dead sperm, sperm concentration, total abnormal sperm, stimulation time and stimulation count were found to be 91.92±21.49 μl, %67.42±4.94, %23.91±8.59, 109.20±66.12x106/ml, %25.57±4.57, 83.08±4.57 sec and 10.35±0.56, respectively. After dissolution, motility and vitality values were found to be, respectively, 45.79±5.49% and 53.57±6.03% with 3% Equiex diluent, 41.78±5.71% and 52.64±6.35% with 6% Equiex diluent, 40.00±5.45% and 51.50±6.72% with Biosfos diluent. After the dissolution, abnormal sperm rates related to the head, middle piece and tail were 3.74±0.91%, 13.62±2.63%, 21.91±4.03%, respectively. In conclusion, approximately 43% motility achieved with the freezing and dissolution of the semen suggests that the semen of Van Cat can be frozen with 43% success. © 2018 IJVS. All rights reserved.