Investigation of Antibiotic Susceptibility and Presence of Plasmids in Staphylococcus Isolated From Cow Milk With Subclinical Mastitis
Abstract
Bu çalışmada Van ve yöresinde yetiştirilen subklinik mastitisli süt ineklerinden alınan 173 adet süt örneğinden izole edilen 51 adet Staphylococcus spp. suşu kullanıldı. İzole edilen suşların 47 tanesinin koagülaz negatif, 4 tanesinin ise koagülaz pozitif olduğu tespit edildi. İzolatların Van bölgesinde mastitis tedavisinde sık kullanılan 6 antibiyotiğe duyarlılıkları belirlendi ve suşlardan plazmit izolasyonu yapıldı. Subklinik mastitisli ineklerden izole edilen 51 adet stafilokok suşundan 3'ünün (% 5.88) oksitetrasikline, 3'ünün (% 5.88) sulfametoksazol-trimetoprim kombinasyonuna, 6'sının (% 11.76) novobiyosin'e, 11'inin (% 21.56) eritromisin'e, 24'ünün (% 47.05) penisilin-G'ye dirençli; 27'sinin (% 52.94) penislin-G'ye, 40'ının (% 78.43) eritromisin'e, 45'inin (% 88.23) novobiyosin'e, 48'sinin (% 94.11) oksitetrasikline, 48'inin (% 94.11) sulfametoksazol-trimetoprim kombinasyonuna, 51'nin (% 100.00) amoksisilin-klavulanik asit kombinasyonuna duyarlı olduğu tespit edildi. İzole edilen suşların %31.37'sinde plazmit tespit edildi. Plazmit taşıyan suşların 1'inin (% 6.25) eritromisine, 2'sinin (% 12.50) oksitetrasikline, 4'ünün (% 25.00) penisilin-G'ye dirençli olduğu, 12'sinin (% 75.00) penisilin-G'ye, 14'ünün (% 87.50) oksitetrasikline, 15'inin (% 93.75) eritromisin'e ve tamamının (% 100.00) novobiyosin, amoksisilin-klavulanik asit ve sülfametoksazol-trimetoprim kombinasyonlarına duyarlı olduğu tespit edildi. Koagülaz negatif suşların 16'sında (% 31.37) plazmit tespit edilmesine rağmen koagülaz pozitif suşlarda plazmit tespit edilemedi. Direnç aktarımında plazmitlerin küçümsenmeyecek bir yeri ve önemi olduğu bilinmekle beraber, plazmit tespit edilemeyen suşlarda da çeşitli antibiyotiklere direnç belirlenmesi nedeniyle antibiyotik direncinin plazmit varlığından kaynaklanıp kaynaklanmadığı anlaşılamadı.Anahtar Kelimeler: Süt, Stafilokok, Antibiyotik direnci, Plazmit.
In this study, 51 Staphylococcus spp. strains that were isolated from 173 milk samples taken from milk cows with subclinic mastitis that are raised in Van and its neighborhood. It was found that 47 of the strains isolated were coagulase-negative and 4 were coagulase-positive. The isolates were found to be susceptible to 6 antibiotics frequently used in mastitis treatment in Van region and plasmid isolation was conducted from the strains.It was found that 3 of the 51 Staphylococcus strains isolated from cows with subclinic mastitis (5.88%) were resistant to oxytetracycline, 3 (5.88 %) to sulphametoxazole- trimethoprim combination, 6 (11.76 %) to novobiocin, 11 (21.56 %) to erythromycin, and 24 (47.05 %) to penicillin-G, and 27 of them (52.94 %) were susceptible to penicillin-G, 40 (78.43 %) to erythromycin, 45 (88.23 %) to novobiocin, 48 (94.11 %) to oxytetracycline, 48 (94.11 %) to sulphametoxazole- trimethoprim combination, and 51 (100.00 %) to amoxicillin- clavulanic acid.Plasmids were detected in 31.37 % of the strains that were isolated. It was found that 1 of the strains that carried plasmids (6.25 %) was resistant to erythromycin, 2 (12.50 %) to oxytetracycline, 4 (25.00 %) to penicillin-G, and 12 of them (75.00 %) were susceptible to penicillin-G, 14 (87.50 %) to oxytetracycline, 15 (93.75 %) to erythromycin, and all of them (100.00 %) to novobiocin, amoxicillin- clavulanic acid, and sulphametoxazole- trimethoprim combination. Although plasmids were found in 16 of the coagulase-negative strains (31.37 %), there were no plasmids found in coagulase-positive strains. It is known that plasmids have a place and importance not to be underestimated in resistance transfer. However, since resistance to certain antibiotics was also detected in strains in which no plasmids were found, it was not understood whether the antibiotic resistance stemmed from the existence of plasmids or not.Keywords: Milk, Staphylococcus, Antibiotic Resistance, Plasmid
In this study, 51 Staphylococcus spp. strains that were isolated from 173 milk samples taken from milk cows with subclinic mastitis that are raised in Van and its neighborhood. It was found that 47 of the strains isolated were coagulase-negative and 4 were coagulase-positive. The isolates were found to be susceptible to 6 antibiotics frequently used in mastitis treatment in Van region and plasmid isolation was conducted from the strains.It was found that 3 of the 51 Staphylococcus strains isolated from cows with subclinic mastitis (5.88%) were resistant to oxytetracycline, 3 (5.88 %) to sulphametoxazole- trimethoprim combination, 6 (11.76 %) to novobiocin, 11 (21.56 %) to erythromycin, and 24 (47.05 %) to penicillin-G, and 27 of them (52.94 %) were susceptible to penicillin-G, 40 (78.43 %) to erythromycin, 45 (88.23 %) to novobiocin, 48 (94.11 %) to oxytetracycline, 48 (94.11 %) to sulphametoxazole- trimethoprim combination, and 51 (100.00 %) to amoxicillin- clavulanic acid.Plasmids were detected in 31.37 % of the strains that were isolated. It was found that 1 of the strains that carried plasmids (6.25 %) was resistant to erythromycin, 2 (12.50 %) to oxytetracycline, 4 (25.00 %) to penicillin-G, and 12 of them (75.00 %) were susceptible to penicillin-G, 14 (87.50 %) to oxytetracycline, 15 (93.75 %) to erythromycin, and all of them (100.00 %) to novobiocin, amoxicillin- clavulanic acid, and sulphametoxazole- trimethoprim combination. Although plasmids were found in 16 of the coagulase-negative strains (31.37 %), there were no plasmids found in coagulase-positive strains. It is known that plasmids have a place and importance not to be underestimated in resistance transfer. However, since resistance to certain antibiotics was also detected in strains in which no plasmids were found, it was not understood whether the antibiotic resistance stemmed from the existence of plasmids or not.Keywords: Milk, Staphylococcus, Antibiotic Resistance, Plasmid
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Mikrobiyoloji, Microbiology
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