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Preparation and Characterization of Cross-Linked Pei-Lipase Aggregates With Improved Activity and Stability

dc.contributor.author Albayrak, Nedim
dc.contributor.author Kanışlı, Mehmet Erhan
dc.date.accessioned 2025-05-10T17:17:56Z
dc.date.available 2025-05-10T17:17:56Z
dc.date.issued 2022
dc.department T.C. Van Yüzüncü Yıl Üniversitesi en_US
dc.department-temp Hi̇ti̇t Üni̇versi̇tesi̇,Van Yüzüncü Yil Üni̇versi̇tesi̇ en_US
dc.description.abstract Using polyethyleneimine (PEI) as the sole precipitation and aggregation agent, PEI-enzyme complexation was investigated with lipases from Rhizomucor miehei, Thermomyces lanuginosus and Candida antarctica. The approach relied on rapid development of PEI-lipase aggregates in a solution and followed by glutaraldehyde cross-linking thus resulting in cross-linked PEI-lipase aggregates. PEI to enzyme mass ratio of a 1/ 20-40 range, alkaline pH and the absence of impurities produced higher coupling yields and activities. The pH affected the precipitatibility and/or relative activity of the aggregates. Impurities in some lipase preparations may prevent the formation or precipitation of the PEI-lipase aggregates. The aggregates attained higher stabilities especially at high pHs and enhanced thermostability with at least a 20-fold at ambient temperatures. By using p-nitrophenyl propionate as a soluble substrate, app. Vmax for the immobilized lipase increased by two-fold with only 25% increment in app. Km compared with the soluble lipase. Complexation with PEI may have produced favorable interface assisting for conformational change for the lipase activation. Thus, cross-linked PEI-lipase aggregates with ease of recovery and stability can be simple and inexpensive alternative for carrier-free immobilized lipases. en_US
dc.identifier.endpage 144 en_US
dc.identifier.issn 2564-6907
dc.identifier.issue 2 en_US
dc.identifier.scopusquality N/A
dc.identifier.startpage 127 en_US
dc.identifier.trdizinid 1150521
dc.identifier.uri https://search.trdizin.gov.tr/en/yayin/detay/1150521/preparation-and-characterization-of-cross-linked-pei-lipase-aggregates-with-improved-activity-and-stability
dc.identifier.uri https://hdl.handle.net/20.500.14720/9495
dc.identifier.volume 5 en_US
dc.identifier.wosquality N/A
dc.language.iso en en_US
dc.relation.ispartof Journal of the Turkish Chemical Society Section B: Chemical Engineering en_US
dc.relation.publicationcategory Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Biyoloji en_US
dc.subject Kimya en_US
dc.subject Uygulamalı en_US
dc.subject Biyoteknoloji Ve Uygulamalı Mikrobiyoloji en_US
dc.title Preparation and Characterization of Cross-Linked Pei-Lipase Aggregates With Improved Activity and Stability en_US
dc.type Article en_US

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