Preparation and Characterization of Cross-Linked Pei-Lipase Aggregates With Improved Activity and Stability
dc.contributor.author | Albayrak, Nedim | |
dc.contributor.author | Kanışlı, Mehmet Erhan | |
dc.date.accessioned | 2025-05-10T17:17:56Z | |
dc.date.available | 2025-05-10T17:17:56Z | |
dc.date.issued | 2022 | |
dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
dc.department-temp | Hi̇ti̇t Üni̇versi̇tesi̇,Van Yüzüncü Yil Üni̇versi̇tesi̇ | en_US |
dc.description.abstract | Using polyethyleneimine (PEI) as the sole precipitation and aggregation agent, PEI-enzyme complexation was investigated with lipases from Rhizomucor miehei, Thermomyces lanuginosus and Candida antarctica. The approach relied on rapid development of PEI-lipase aggregates in a solution and followed by glutaraldehyde cross-linking thus resulting in cross-linked PEI-lipase aggregates. PEI to enzyme mass ratio of a 1/ 20-40 range, alkaline pH and the absence of impurities produced higher coupling yields and activities. The pH affected the precipitatibility and/or relative activity of the aggregates. Impurities in some lipase preparations may prevent the formation or precipitation of the PEI-lipase aggregates. The aggregates attained higher stabilities especially at high pHs and enhanced thermostability with at least a 20-fold at ambient temperatures. By using p-nitrophenyl propionate as a soluble substrate, app. Vmax for the immobilized lipase increased by two-fold with only 25% increment in app. Km compared with the soluble lipase. Complexation with PEI may have produced favorable interface assisting for conformational change for the lipase activation. Thus, cross-linked PEI-lipase aggregates with ease of recovery and stability can be simple and inexpensive alternative for carrier-free immobilized lipases. | en_US |
dc.identifier.endpage | 144 | en_US |
dc.identifier.issn | 2564-6907 | |
dc.identifier.issue | 2 | en_US |
dc.identifier.scopusquality | N/A | |
dc.identifier.startpage | 127 | en_US |
dc.identifier.trdizinid | 1150521 | |
dc.identifier.uri | https://search.trdizin.gov.tr/en/yayin/detay/1150521/preparation-and-characterization-of-cross-linked-pei-lipase-aggregates-with-improved-activity-and-stability | |
dc.identifier.uri | https://hdl.handle.net/20.500.14720/9495 | |
dc.identifier.volume | 5 | en_US |
dc.identifier.wosquality | N/A | |
dc.language.iso | en | en_US |
dc.relation.ispartof | Journal of the Turkish Chemical Society Section B: Chemical Engineering | en_US |
dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Biyoloji | en_US |
dc.subject | Kimya | en_US |
dc.subject | Uygulamalı | en_US |
dc.subject | Biyoteknoloji Ve Uygulamalı Mikrobiyoloji | en_US |
dc.title | Preparation and Characterization of Cross-Linked Pei-Lipase Aggregates With Improved Activity and Stability | en_US |
dc.type | Article | en_US |