Evaluation of Dose-Dependent Effects of Nesfatin-1 in Pentyleneterazole Induced Seizures in Mice

Abstract

Objectives: It is well known that neuropeptides have powerful modulator effects on inhibitory and excitatory systems in the brain. Serum nesfatin-1 levels are reported to be increased in epileptic patients and experimental models of epilepsy. However, the effects of nesfatin-1 administration on acute pentylenetetrazole (PTZ)-induced seizures are unknown. The aim of this study is to investigate the dose-dependent effects of nesfatin-1 in PTZ-induced acute seizure model in mice. Methods: Animals were divided into six groups: (1) Control, (2) PTZ, (3, 4, 5, 6) PTZ+Nesfatin-1 (1, 10, 20 veya 40 mu g/kg, i.p). Physiological saline (PS, 1 mL/kg, i.p) was administrated to the mice in the control group. Nesfatin-1 or PS was administered to the experimental groups. Thirty minutes after injection of nesfatin-1 or SF, PTZ (80 mg/kg, i.p) was injected into all experimental groups to induce epileptic seizures. Latencies of the first myoclonic jerk (FMJ), generalized clonic seizures (GCS), and tonic generalized extension (TGE), and duration of TGE were determined after PTZ injection. Results: No significant difference was found between the groups in terms of latencies of FMJ, GCS, TGE, and duration of TGE. Conclusion: Nesfatin-1 did not show anti or pro-convulsant effects at all doses. In conclusion, our data demonstrated that nesfatin-1 administration had no anti/pro-convulsant effect on PTZ (80 mg/kg) induced acute seizures in mice.