Identification of Genetic Variations on Fritillaria Imperialis L. Genotypes Collected From Van Lake Basin by Ipbs-Retrotranspozon Markers

Abstract

Fritillaria imperialis L. naturally grows in Turkey and it is used as an ornamental plant. This species harbored plentiful genetic variation for various morphological traits in its natural habitat. In this study, 19 different iPBS-Retrotransposon primers were used to identify genetic variation among 74 F. imperialis genotypes collected around Van lake basin. 19 primers amplified 94 bands, 100% polymorphism. The average number of bands per primer was 4.94 and the average polymorphism information content (PIC) was 0.58. Maximum number of polymorphic bands were 10 while the minimum number of polymorphic bands were 2. The mean effective number of alleles, Shannon information index and the gene diversity were 1.50, 0.47, and 0.30 respectively. The results reflected that genetic variations of F. imperialis genotypes collected from Van lake basin were significant. This is the first report identifying the genetic variations of F. imperialis genotypes by iPBS-Retrotransposon primers, and it proved that iPBS-Retrotransposon marker system could be applied successfully in F. imperialis for genetics and genomic studies. The data obtained from this study will provide preliminary information for future F. imperialis breeding activities. © 2020, Centenary University. All rights reserved.