Determination of Serotypic Differences of Lactococcus Garvieae Isolates Obtained From Rainbow Trout Farms

Abstract

This study aimed to determine the serotypic differences between Lactococcus garvieae strainsisolated from rainbow trout obtained from different fish farms in Turkey. For this purpose, somephenotypic properties of isolates were determined and then ELISA test was performed to determine serotypic differences. It was determined that all 22 L. garvieae isolates used in the study gavea cream colored, bright, rounded smooth S type colony in Triptic Soy Agar (TSA) and all strainswere nonmobile in the native examination. Morphologically, all isolates were found to be Grampositive, nonmobile, α-hemolytic, to have a growth of 0-6.5% NaCl salinity, 21, 37 and 45℃temperature, and to be oxidase and catalase negative. After examining the biochemical made withAPI Rapid ID 32 Strep test, it was observed that two L. garvieae isolates were different from theother isolates in terms of sucrose use and one L. garvieae isolate was different from other isolatesin terms of maltose profile. According to the results of ELISA test used to determine the serotypical differences of the isolates, two L. garvieae isolates were serotypically different from the otherisolates and L. garvieae isolates used in the study formed two different serotypic groups.