Biochemical Marker Levels in Peri-Implant Crevicular\rfluid of Completely Edentulous Patients With Two\rimplant-Supported Mandibular Overdentures Before\rand 6 Months After Loading: Prospective Clinical Trial

Abstract

Aim: The aim of this study was to determine the levels of biochemical\rmarkers detected in the peri-implant crevicular fluid (PICF) before and 6\rmonths after loading in completely edentulous patients with two implantsupported mandibular overdentures and to correlate them with clinical\rparameters.\rMethodology: Two implants were applied to the mandibular canine\rregion of the 20 completely edentulous and systemically healthy patients\r(11 females, 9 males) with conventional 2-stage surgery. PICF samples of\rthe participants were collected before and 6 months after prosthetic\rloading, and clinical parameters—including modified plaque index (MPI),\rgingival index (GI), bleeding on probing (BoP), probing pocket depth (PPD),\rand keratinized gingival width (KGW)—were recorded. Interleukin-1β (IL1β), tumor necrosis factor-α (TNF-α), matrix metalloproteinase-8 (MMP-8),\rand aspartate aminotransferase (AST) levels were examined in PICF\rsamples via enzyme-linked immunosorbent assay (ELISA).\rResults: The average age of the participants was 57.05 ± 9.82 years.\rAlthough a decrease was observed in all clinical parameters after\rprosthetic loading compared to baseline levels, this decrease was not\rconsidered statistically significant (p>0.05). PICF volume showed a\rstatistically significant decrease from baseline to 6 months (p<0.05). The\rdecreases observed at 6th month values in all of the biochemical markers\rexamined were not found to be statistically significant, similar to the\rclinical parameters (p>0.05).\rConclusion: Within the limits of this study, the PICF marker levels and\rclinical parameters determined following osseointegration and 6 months\rafter prosthetic loading are similar. This suggests that the studied sample\rhas prosthetic restorations that contribute positively to the tissue\rmaturation process and apply ideal forces to the tissue. More\rcomprehensive studies are recommended to understand the tissue healing\rprocess after implant treatment.