The Incidence of Barley Yellow Dwarf Viruses (Bydvs) in Wheat Crops in Diyarbakir (Turkey) and Sequence Characterization of Bydv-Pav

Abstract

To ascertain the presence of Barley/Cereal yellow dwarf viruses (BYDV-PAV, MAV, SGV, RMV,and CYDV-RPV), a total of 365 wheat leaf samples were randomly collected from wheat cultivation fields ofDiyarbakir province in 2016 and were screened by using multiplex RT-PCR and RT-PCR methods. As a resultof PCR tests the wheat samples were found to be infected by BYDV-PAV, BYDV-SGV and CYDV-RPV withthe percentage of 3.5%, 2.4% and 1.3%, respectively. Detected mix infections of BYDV-PAV+BYDV-SGV,BYDV-PAV+CYDV-RPV, BYDV-SGV+CYDV-RPV, and BYDV-PAV+BYDV-SGV+CYDV-RPV were2.2%, 0.8%, 0.8%, and 0.8%, respectively. A BYDV-PAV Diyarbakir isolate was chosen randomly, and itscomplete coat protein gene was characterized. The coat protein gene of BYDV-PAV virus (Genbank accessionno. KX774424) was molecularly cloned and sequenced with the universal primers. The CP nucleotide sequenceof BYDV-PAV Diyarbakir isolate was showed 81.4 - 98.3% identities with the other 21 world isolates. Thepresent study is the first report for documentation of BYDV-PAV, BYDV-SGV and CYDV-RPV viruses inwheat fields of Diyarbakir province (Turkey).