Purification and Characterization of Van Lake Fish (Chalcalburnus Tarichii P.1811) Liver and Brain Acethylcholinesterase
| dc.contributor.author | Akman, Esrin | |
| dc.contributor.author | Türkoğlu, Vedat | |
| dc.contributor.author | Celik, Ismail | |
| dc.date.accessioned | 2025-05-10T16:50:06Z | |
| dc.date.available | 2025-05-10T16:50:06Z | |
| dc.date.issued | 2009 | |
| dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
| dc.department-temp | Van Yüzüncü Yil Üni̇versi̇tesi̇,Van Yüzüncü Yil Üni̇versi̇tesi̇,Van Yüzüncü Yil Üni̇versi̇tesi̇ | en_US |
| dc.description.abstract | In this study, acethylcholinesterase was purified from liver and brain of Van Lake fish (Chalcalburnus Tarichii P.1811) by using affinity chromatography technique. The purification folds were determined as 142 and 344 for brain and liver, respectively. The purity of enzyme was controlled with sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDSPAGE). The optimal pH was determined as 7.5-8.5 for brain and liver, respectively. On the other hand, the optimal temperature was determined as 35oC for both liver and brain. Also, the highest activities were observed in concentration of 0.2 M (NH4)2SO4 as ionic strength for both liver and brain. | en_US |
| dc.identifier.endpage | 336 | en_US |
| dc.identifier.issn | 1303-5002 | |
| dc.identifier.issn | 2687-475X | |
| dc.identifier.issue | 4 | en_US |
| dc.identifier.scopusquality | N/A | |
| dc.identifier.startpage | 331 | en_US |
| dc.identifier.trdizinid | 103866 | |
| dc.identifier.uri | https://search.trdizin.gov.tr/en/yayin/detay/103866/purification-and-characterization-of-van-lake-fish-chalcalburnus-tarichii-p1811-liver-and-brain-acethylcholinesterase | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14720/2083 | |
| dc.identifier.volume | 37 | en_US |
| dc.identifier.wosquality | N/A | |
| dc.language.iso | en | en_US |
| dc.relation.ispartof | Hacettepe Journal of Biology and Chemistry | en_US |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Biyoloji | en_US |
| dc.subject | Balıkçılık | en_US |
| dc.subject | Deniz Ve Tatlı Su Biyolojisi | en_US |
| dc.subject | Fizyoloji | en_US |
| dc.subject | Biyokimya Ve Moleküler Biyoloji | en_US |
| dc.title | Purification and Characterization of Van Lake Fish (Chalcalburnus Tarichii P.1811) Liver and Brain Acethylcholinesterase | en_US |
| dc.type | Article | en_US |