Plasma Macro and Trace Element Levels of Male Rats Vaccinated With Gnrh Hormone

Abstract

GnRH hormone is important in both male and female reproduction. Chemical agents are preferred to prevent fertility in some animals. Trace elements play an important role in reproduction. In this study, it was aimed to investigate the changes in macro- and trace element levels in blood plasmas of 6th and 12th months of GnRH vaccination (Repro-Bloc (TM)) male rats. 50 male Wistar Albino rats were used in the study. Each rat in the experimental group was administered a single 200 mu l intramuscular dose of GnRH. The levels of Cu, Fe, Mn, Zn, Se, K, P, Ca, and Mg elements were determined by analyzing the collected plasma samples using inductively coupled plasma-optical emission spectroscopy (ICP-OES). It was determined that zinc, manganese and selenium levels of the trace elements showed statistically significant changes in the first 6 months and Cu and Fe levels were statistically significant (P<0.05). In the twelfth month, it was observed that the levels of Fe, Mn, Se and Zn trace elements differ statistically. In the macro elements, K, P elements in the first 6 months period and Ca, Fe and Mg element levels in 16 months period gave statistically significant results (P<0.05). Conclusions: It was observed that levels of macro and micro elements were affected in male rats administered GnRH vaccine for immunocastration