Analysis of Antibiotic Resistance Among Kpc-3 Positive Pseudomonas Aeruginosa Strains Isolated From Intensive Care Unit Patients

Abstract

The increase in antibiotic resistance among microorganisms is an issue of utmost importance. Specifically, the resistance to carbapenem and extended-spectrum β-lactam antibiotics in gram-negative bacteria has had a considerable impact on patient morbidity and mortality rates. This research is centered around the examination of severe infections caused by Pseudomonas aeruginosa and KPC-3 in patients who have been admitted to intensive care units. P. aeruginosa strains were collected from the intensive care units of Van Training and Research Hospital. In order to conduct a thorough microbiological analysis of these strains, various methods including culture, biochemical tests, antibiogram tests, and polymerase chain reaction (PCR) were employed. A total of 126 carbapenem-resistant P. aeruginosa strains were extracted from patients within intensive care units. Among these isolates, 17 were identified as being both multidrug-resistant and positive for KPC-3. Interestingly, all of the identified strains exhibited sensitivity to Amikacin, Piperacillin, Piperacillin/Tazobactam, Gentamicin, Colistin, Fosfomycin and Tigecycline. The identification of KPC-3 positive P. aeruginosa strains among patients in our hospital highlights a concerning issue. It has been observed that this presence increases the risk of both mortality and morbidity in affected patients. Consequently, it has been deemed crucial to address this matter in terms of hospital surveillance practices and the implementation of strategies to combat antibiotic resistance. © 2025, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.