Antioxidant and Anti-Lipoxygenase Activities of Cydonia Oblonga

Abstract

The present studies are focused to explore the antioxidant and anti-lipoxygnease activities in crude extract, aqueous and organic fractions (ethyl acetate, chloroform, butanol) of dry fruit of Cydonia oblonga. For determining the antioxidant activity; total phenolic content and 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay were performed in the aqueous and organic fractions of C. oblonga. In our study, phenolic content revealed that crude extract has highest phenolic content followed by ethyl acetate fraction. The results of DPPH radical scavenging activity showed IC50 values of crude extract as 61.4 μg/mL, chloroform and aqueous 67.4 and 55.5 μg/mL, respectively, which are well comparable with butylatedhydroxyanisol (BHA) standard, which showed IC50 as 44.2 μg/mL. In addition, crude extract appeared to be the prominent inhibitor of lipoxygenase. The results suggests that the extract and different fractions of dry fruit of C. oblonga exhibits significant antioxidant and free radical scavenging activities/capacity which might be associated with the presence of main phytochemicals like flavonoids, phenols and tannins. Besides that, all these extract and fractions have well enough lipoxygenase inhibitory potential. As a result, we can claim that the presence of the scavenger molecules in the dry fruit of C. oblonga may attribute health benefits by providing the protective effects during the progressive stages of different acute and chronic disorders.