Browsing by Author "Akgul, O."

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Analysis of Antibiotic Resistance Among Kpc-3 Positive Pseudomonas Aeruginosa Strains Isolated From Intensive Care Unit Patients
(Yuzuncu Yil Universitesi Tip Fakultesi, 2025) Akgul, O.; Bora, G.
The increase in antibiotic resistance among microorganisms is an issue of utmost importance. Specifically, the resistance to carbapenem and extended-spectrum β-lactam antibiotics in gram-negative bacteria has had a considerable impact on patient morbidity and mortality rates. This research is centered around the examination of severe infections caused by Pseudomonas aeruginosa and KPC-3 in patients who have been admitted to intensive care units. P. aeruginosa strains were collected from the intensive care units of Van Training and Research Hospital. In order to conduct a thorough microbiological analysis of these strains, various methods including culture, biochemical tests, antibiogram tests, and polymerase chain reaction (PCR) were employed. A total of 126 carbapenem-resistant P. aeruginosa strains were extracted from patients within intensive care units. Among these isolates, 17 were identified as being both multidrug-resistant and positive for KPC-3. Interestingly, all of the identified strains exhibited sensitivity to Amikacin, Piperacillin, Piperacillin/Tazobactam, Gentamicin, Colistin, Fosfomycin and Tigecycline. The identification of KPC-3 positive P. aeruginosa strains among patients in our hospital highlights a concerning issue. It has been observed that this presence increases the risk of both mortality and morbidity in affected patients. Consequently, it has been deemed crucial to address this matter in terms of hospital surveillance practices and the implementation of strategies to combat antibiotic resistance. © 2025, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Analysis of Antibiotic Resistance Profiles of Gram-Positive Bacteria Isolated From Blood Culture Samples in Patients With Catheter Infection
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Akgul, O.; Bora, G.
Bloodstream enfections, which is common and often lethal, is a serious public health problem. There is consensus that the incidence is increased in patients with sepsis due to an aging population, increased use of immunosuppressi ve therapy, and high-risk interventions. In this study, 750 patients with suspected sepsis hospitalized in intensive care units in the Health Education University Training and Research Hospital were evaluated. 750 patients were classified according to thei r age and sex. Bacteria were isolated from their blood cultures. Biochemical tests such as catalase test, oxidase test, Gram staining and Coagulaz test were performed. Vitek 2 Compact (Biomerieux, USA) device was used for identification of bacteria and eva luation of the antibiogram test. The mecA genes were analyzed by polymerase chain reaction (PCR). Two hundred and four Gram-positive bacteria from 750 (375 male and 375 female) were isolated in the blood culture samples taken from the intensive care units. 101 (26.9%) and 103 (27.5%) bacteria were isolated from 375 each male and female patients, respectively. Slime factor was positive in 47 of the coagulase negative bacteria. Twelve methicillin resistant Staphylococcus aureus (MRSA) isolates were positive for mecA gene carriage. Identification of the bacteria causing sepsis in our hospital and determining the antibiotic resistance rates were found quite important. Among the causative agents of sepsis, effect of the presence of methicillin and multidrug resistance bacteria on humman health were found to be very important. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Molecular Analysis of Oxa-48 Producing K. Pneumoniae Strains Isolated From Patients With Catheter-Associated Sepsis Br
(verduci Publisher, 2022) Akgul, O.; Bora, G.
OBJECTIVE: K. pneumoniae is an important cause of hospital and community-acquired infections. In particular, carbapenem-resistant strains of K. pneumoniae spread globally, increasing the public health risk. This study aims to sequence and phylogenetically analyze K. pneumoniae strains isolated from blood cultures of patients in intensive care units in our hospital. MATERIALS AND METHODS: In this study, blood samples were collected from patients with catheter-related sepsis. Culture, biochemical, antibiotic susceptibility, and molecular tests were performed as microbiological analyses. RESULTS: Twenty-four K. pneumoniae strains showing multidrug resistance by isolating 276 K. pneumoniae were included in the study. It was determined that they showed the highest resisAcid, Ceftazidime, and Ceftriaxone. The comparison determined that K. pneumoniae isolates from different countries isolated from blood cultures had closeness and distance in OXA-48. CONCLUSIONS: After multilocus sequence typing, all of our 24 K. pneumoniae isolates were determined to be ST11.
Phenotypic and Genotypic Analysis of the Antibiotic Resistance Profiles of Gram Negative Bacteria Isolated From the Blood Culture Samples
(Yuzuncu Yil Universitesi Tip Fakultesi, 2021) Akgul, O.; Bora, G.; Kustan, A.
Sepsis, which is common and often lethal, is a serious public health problem. There is consensus that the incidence is increased in patients with sepsis due to an aging population, which leads to an increase in the use of immunosuppressive therapy, and high-risk interventions. Seven hundred fifty patients with suspected sepsis hospitalized in intensive care units in the Health Education University Van Training and Research Hospital were evaluated. These patients were classified according to their age and sex. Bacteria were isolated from their blood cultures. Biochemical tests such as catalase, oxidase tests and Gram staining were performed. Vitek 2 Compact (Biomerieux, USA) device was used for identification of bacteria and evaluation of the antibiogram test. The blaOXA-48 and blaIMP genes were analyzed by polymerase chain reaction (PCR) for those which display multidrug resistance to certain antibiotics. One hundred and sixteen Gram negative bacteria were isolated. Among them, 44 Klebsiella pneumoniae (37.9%), 40 Acinetobacter baumannii (34.5%), 27 Escherichia coli (23.3%) and 5 Pseudomonas aeruginosa (4.3%) were identified. Eleven multidrug-resistant bacteria were assessed by Vitek 2 Compact device. Among them, two K. pneumoniae isolates were found to be blaOXA-48 carriers, whereas blaIMP gene was not found in any of eleven isolates. The presence of the bacteria with carbapenem, extended β-lactamase and multidrug resistance among the infectious bacteria may create a risk for human health. The risk factors may vary depending on age and gender in patients with sepsis and bloodstream infections. It was concluded that surveillance reports should be regulated according to this fact. © 2021, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Serum Homocysteine Levels in Calves With Foot and Mouth Disease
(Medwell Journals, 2013) Kaya, A.; Kozat, S.; Ozkan, C.; Yildirim, S.; Akgul, Y.; Akgul, O.
The association between serum Homocysteine (Hey) and myocarditis in calves with Foot and Mouth Disease (FMD) are not well defined. To investigate the relationship between serum Hey concentrations and myocarditis in healthy calves and calves with FMD. A total of 9 healthy calves (control) and 15 calves with FMD. In the study, all animals underwent a comprehensive clinical and laboratuar findings study to document cardiac health or presence of FMD. Blood samples were collected via jugular venipuncture. Serum Hey, Nitric Oxide (NO), cardiac Troponin I (cTnl) concentrations, serum levels of activity Creatine Kinase (CK), Lactac Dehydrogenase (LDH) and cardiac origin Creatine Kinase (CK-MB) were measured. Serum Hey, NO, cTnl, CK, CK-MB and LDH values in calves with FMD significantly higher compared to control (p<0.01, p<0.001, p<0.01, p<0.001, p<0.001 andp<0.05, respectively). The results suggest that determination of serum Hey concentrations in calves with clinical signs compatible with FMD might prove useful as a guide to quantify cardiac remodeling associated with heart damage. © Medwell Journals, 2013.