Analysis of Antibiotic Resistance Profiles of Gram-Positive Bacteria Isolated From Blood Culture Samples in Patients With Catheter Infection

Abstract

Bloodstream enfections, which is common and often lethal, is a serious public health problem. There is consensus that the incidence is increased in patients with sepsis due to an aging population, increased use of immunosuppressi ve therapy, and high-risk interventions. In this study, 750 patients with suspected sepsis hospitalized in intensive care units in the Health Education University Training and Research Hospital were evaluated. 750 patients were classified according to thei r age and sex. Bacteria were isolated from their blood cultures. Biochemical tests such as catalase test, oxidase test, Gram staining and Coagulaz test were performed. Vitek 2 Compact (Biomerieux, USA) device was used for identification of bacteria and eva luation of the antibiogram test. The mecA genes were analyzed by polymerase chain reaction (PCR). Two hundred and four Gram-positive bacteria from 750 (375 male and 375 female) were isolated in the blood culture samples taken from the intensive care units. 101 (26.9%) and 103 (27.5%) bacteria were isolated from 375 each male and female patients, respectively. Slime factor was positive in 47 of the coagulase negative bacteria. Twelve methicillin resistant Staphylococcus aureus (MRSA) isolates were positive for mecA gene carriage. Identification of the bacteria causing sepsis in our hospital and determining the antibiotic resistance rates were found quite important. Among the causative agents of sepsis, effect of the presence of methicillin and multidrug resistance bacteria on humman health were found to be very important. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.