Browsing by Author "Boyaci, Ismail Hakki"

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The Comparison of Antioxidant Capacity and Cytotoxic, Anticarcinogenic, and Genotoxic Effects of Fe@au Nanosphere Magnetic Nanoparticles
(Tubitak Scientific & Technological Research Council Turkey, 2017) Yegenoglu, Hande; Aslim, Belma; Guven, Burcu; Zengin, Adem; Boyaci, Ismail Hakki; Suludere, Zekiye; Tamer, Ugur
Magnetic gold nanoparticles are used in various biomedical, biochemistry, and biotechnology applications due to their controllable size distribution, long-term stability, reduced toxicity, and biocompatibility. Different coating materials, such as proteins, carbohydrates, lipids, and polyphenols, are applied to enhance the biocompatibility of nanoparticles. In this study, the effects of surface coatings of core-shell structured Fe@Au nanosphere magnetic nanoparticles with regard to antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic properties were investigated. The obtained results demonstrated that avidin-coated Fe@Au nanospheres had higher antioxidant capacities than uncoated nanospheres. Neither avidin-coated nor uncoated nanoparticles had a cytotoxic effect on normal cells (human gingival fibroblast cell line, HGF-1). In addition, they had anticarcinogenic effects on human cervical carcinoma (HeLa), human breast adenocarcinoma (MCF-7), and human colorectal adenocarcinoma (CCL-221). The genotoxic effects of nanoparticles were also evaluated with DNA tail damage ratio.
Dual Responsive Disposable Electrode for the Enumeration Ofescherichia Coliin Whole Blood
(Wiley-v C H verlag Gmbh, 2020) Panhwar, Sallahuddin; Ilhan, Hasan; Hassan, Syeda Sara; Zengin, Adem; Boyaci, Ismail Hakki; Tamer, Ugur
In this report, we have designed a dual responsive disposable electrode for the enumeration ofEscherichia coli K12 (E. coli K12). The immunomagnetic separation strategy provided easily bacterial detection in the whole blood. Metal-organic frameworks magnetic (MOFs) modified, and citrate capped gold nanoparticles were used as capture probe and spectro-electrochemical labels, respectively. The cyclic voltammetry (CV) was employed to quantifyE. coliwith a ranging from 10(1)to 10(7) cfu/mL with a LOD of 1 cfu/mL. The Surface enhanced Raman spectroscopy (SERS) measurements were also performed with using disposable electrode.
Rapid Bacterial Detection Through Microfluidic Integration With a Glucometer
(Elsevier Science Sa, 2025) Eryilmaz, Merve; Ilbasmis-Tamer, Sibel; Panhwar, Sallahuddin; Tayyarcan, Emine Kubra; Boyaci, Ismail Hakki; Suludere, Zekiye; Tamer, Ugur
We present a novel approach for sensitive and portable detection of pathogenic bacteria, which is crucial for household and clinical practice. Our method employs immunoliposomes, antibodies, and microchip to detect specific pathogens quantitatively. Gold and metal metal-organic nanoparticles and liposomes were characterized using high-resolution techniques like TEM and SEM. Utilizing a commercial, personal glucose meter (PGM), we initially detected released glucose from antibody-modified liposomes and microchips with MOF-NPs. Detection on the microchip was achieved within 30 min, while the PGM analysis took only one minute for targeted bacteria, yielding glucose signals of 66 mg/dL and 69 mg/dL, respectively. Serial dilutions with group A-Streptococcus pyogenes (GAS) (1.4 x 10<<^>>4-1.4 x 10<<^>>8 CFU/mL) demonstrated quantitative measurement applicability. This innovative approach and a portable PGM hold promise for various industries, including physician labs, hospitals, and households.
Rapid Quantification of Total Protein With Surface-Enhanced Raman Spectroscopy Using O-Phthalaldehyde
(Wiley, 2017) Eryilmaz, Merve; Zengin, Adem; Boyaci, Ismail Hakki; Tamer, Ugur
In this study, surface-enhanced Raman spectroscopy (SERS) based quantification method for the total protein using o-phthalaldehyde is reported for the first time. For this purpose, o-phthalaldehyde was chosen to form a complex with protein, and SERS signal was observed in the presence of gold nanoparticles. A calibration curve was obtained by plotting the intensity of the SERS signal at 727cm(-1)versus the concentration of protein standard (bovine serum albumin, BSA). Thus, the correlation was found to be linear within the range of 0.054-0.72mg/ml of BSA, and the limit of detection was determined to be 0.08mg/ml. All tests were carried out using a portable Raman instrument with an analysis time of 5min. In addition, the ability of the developed method to quantify total protein in milk samples was investigated, and the obtained results were compared to the conventional ultraviolet methods. Copyright (c) 2017 John Wiley & Sons, Ltd.