Browsing by Author "Comba, Bahat"

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Advanced Oxidation Protein Products (Aopp) Levels and Kidney Function in Fluorotic Sheep
(int Soc Fluoride Research, 2016) Mert, Handan; Comba, Bahat; Mert, Nihat; Cinar, Ali; Apaydin, Betul
The ingestion of excessive amounts of the fluoride ion (F) may cause chronic fluorosis with abnormalities in many organs and systems including, in addition to dental and skeletal fluorosis, impaired renal function. The aim of the present study was to investigate renal function and the levels of advanced oxidation protein products (AOPP) as oxidative stress markers in chronically fluorotic sheep with kidney disease. Fifteen healthy and 30 fluorotic sheep were studied. The plasma F levels were determined with a F-selective electrode. The plasma AOPP and the serum total protein levels were measured spectrophotometrically. The serum BUN, creatinine, potassium, sodium, and chloride levels were determined by autoanalyzer. Compared to the control group, significant increases were present in the fluorotic sheep in the levels of F (p=0.001), AOPP (p=0.001), BUN (p=0.001), creatinine (p= 0.05), and total protein (p= 0.05). A significant decrease was present in the fluorotic sheep in the level of potassium (p=0.01) while no significant changes were present in the sodium and chloride concentrations. The levels of AOPP in chronically fluorotic sheep, reported here for the first time in veterinary science, and the biochemical abnormalities can be considered in the evaluation of the effects of chronic fluorosis on kidney function.
Arpa Çiminin Diyabetli Ratlarda Antioksidan Kapasitesine ve Dna Hasarına Etkisi
(2023) Başbuğan, Yıldıray; Comba, Bahat; Aksu, Devrim Sarıpınar; Comba, Arzu; Mis, Leyla
Amaç: Dünya çapında fitoterapi yöntemleri büyük önem kazanmakta ve bu alandaki çalışmalar her geçen gün önemini artırmaktadır. Bu çalışmada deneysel olarak diyabet oluşturulan ratlarda toplam antioksidan, oksidan durum, oksidatif DNA hasarı, glikoz ve hemoglobin A1c düzeyleri ve arpa çimi suyunun bu parametreler üzerine etkisinin incelenmesi amaçlandı. Gereç ve yöntem: Çalışma için 4 grup oluşturuldu ve her grupta 250-350 gr ağırlığında 6 erkek rat kullanıldı. Kontrol grubu; serum fizyolojik periton içi yol ile enjekte edildi, Diyabet grubu; diyabet, streptozotosin kullanılarak oluşturuldu, Arpa çimi grubu; 4 hafta oral olarak arpa çimi suyu (3 ml/sıçan/gün) verildi, Diyabet+Arpa çimi grubu; streptozotosin ile diyabet oluşturuldu ve 4 hafta boyunca arpa çimi suyu oral olarak verildi. Bulgular: Arpa çimi suyu, streptozotosin kaynaklı diyabette kan şekeri, glikoz, HemoglobinA1c, toplam oksidatif durum ve oksidatif stres gösterge değerlerini düşürdüğü, toplam antioksidan değerini ve canlı ağırlıkları arttırdığı belirlendi. Ayrıca arpa çimi suyu ilavesinin bu parametreler üzerinde belirgin koruyucu etki ve iyileşme sağladığı tespit edildi. Sonuç: Bu bulgulardan yola çıkarak arpa çimi suyunun şeker hastalığında anti-diyabetik, anti-oksidan etki gösterdiği ve kilo kaybını önlediğini söyleyebiliriz.
Assessment of the Wheatgrass Effects on Antioxidant Capacity and Dna Damage in Rats With Experimental Renal Failure
(Parlar Scientific Publications (p S P), 2019) Mis, Leyla; Comba, Bahat; Basbugan, Yildiray; Yasar, Semih; Comba, Arzu
In this study, it was aimed to evaluate the effect of wheatgrass on antioxidant capacity and DNA damage in rats with renal failure. In the study, 200-300 g 24 healthy Wistar-Albino male rats obtained from YYU Experimental Animal Unit were used. The rats were randomly divided into 4 groups and each group was formed with 6 rats. Groups were allocated as; 1. Control Group, 2. Renal Failure Group (Genta Group) 3. Renal Failure + Wheatgrass Group (Genta +Wheatgrass) 4. Wheatgrass Group. Gentamicin sulfate was given intraperitoneal at a dose of 50 mg/kg for 1 week and once a day to the groups with renal failure (Groups 2 and 3). Wheat grass extract (10 ml/kg) was added to the drinking water of the wheatgrass group rats (3rd and 4th groups) once a day. This practice lasted for 4 weeks. Total oxidant, antioxidant quantities, DNA damage were evaluated at the end of 4 weeks. The result that wheatgrass could be useful in renal failure was reached.
The Effect of Barley Grass on Antioxidant Capacity and Dna Damage in Rat With Renal Failure
(Parlar Scientific Publications (p S P), 2017) Comba, Bahat; Mis, Leyla; Uslu, Sema; Comba, Arzu
This study was aimed to search the effect of barley grass (BG) on the Total Antioxidant (TAS)-Oxidant Status (TOS) and DNA damage (8OHdG) in rat with renal failure. The rats which are used in the study were randomly divided into 4 groups that each of has 8 rats: Control (C) group; injected i.p. with physiological saline once a day for 7 days, GM group; Gentamicin (80 mg/kg/day) was injected i.p. for 7 days, BG group; was given oral BG (250 mg/kg/day) for 4 weeks, GM+BG group was injected gentamicin (80 mg/kg/day) with i.p. for 7 days and BG (250 mg/kg/day) was given by oral for 4 weeks. After from the process of experiment for 4 weeks, blood sample and kidney tissue were taken. The analyses of urea and creatinine were done by autoanalyser; TAS, TOS levels by colorimetric kits; 8OHdG level by ELISA kits in serum. The kidney tissues were examined histologically. In the GM+BG group was statistically decreased levels of urea, creatinine, TOS (p<0.05) and OSI (p<0.01) compared to GM group. In the group of BG was determined the levels of TAS p<0.05 statistically increased other groups. The statistical significance was not found in the level of serum 8OHdG differences between the groups. BG decreased widespread damage to the renal glomerulus and proximal tubulus. As a result, adverse effects of high dose gentamicin application such as kidney damage can be reduced when used barley grass.
The Effect of Chemical and Surgical Castration on the Live Weight Gain and Some Hormones of Male Norduz Sheep
(Zoological Soc Pakistan, 2017) Karakus, Kadir; Comba, Bahat; Tas, Abuzer; Sancak, Tunahan; Comba, Arzu; Aksu, Devrim Saripinar; Tariq, Mohammad Masood
The aim of this study was to investigate the effects of surgical and chemical castration on body weight and some hormones. In the present study, 2.5-3-month-old weaned male Norduz sheep were used. The sheep were obtained in the year 2015 from Livestock Application and Research Directorate of Yuzuncu Yil University. The sheep were randomly allocated into three groups each of which contained 10 sheep. The groups were formed as; Group 1 The Control Group (No castration), Group 2 (Surgical castration group) and Group 3 (Chemical castration group), respectively. Blood sample was taken from vena jugularis of the animals before the application and every two months for a total of 4 times for 6 months and hormone analyses were performed. The order of statistical significance for TSH (thyroid stimulating hormone) were found as Surgical > Control > Formol (P<0.05). The order of statistical significance for growth hormone was Formol = Surgery > Control (P<0.05). In August month, the application factor was found to be significant only in testosterone (P<0.05). No significant difference between the control and surgical groups in testosterone was noted. In this study, although the application of different castration methods in 4-month-old male Norduz sheep resulted in changes in the blood hormone levels, it was determined that these changes did not affect body weight. In conclusion, the evaluation of the process in castrated six-month-old sheep revealed an increase in the body weight which was similar to that in the control group.
The Effect of Cinnamaldehyde on The Activity of Glucose-6 Dehydrogenase, Some Biochemical and Hematological Parameters in Diabetic Rats
(Wiley, 2017) Celik, Remzi; Mert, Handan; Comba, Bahat; Mert, Nihat
Effect of Wheatgrass on Dna Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats
(Soc Chilena Anatomia, 2018) Mis, Leyla; Comba, Bahat; Uslu, Sema; Yeltekin, Asli
This study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 mlikg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001),TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.
Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene
(Kafkas Univ, veteriner Fakultesi dergisi, 2016) Comba, Bahat; Oto, Gokhan; Mis, Leyla; Ozdemir, Hulya; Comba, Arzu
In this study was investigated effects of borax (BX) on inflammation markers, haematolojical parameters and total oxidant (TOS)-antioxidant status (TAS) in rats applied 3-methylcholanthrene (3-MC). In this research a total of 24 Wistar Albino rats were used. They were divided into 4 groups each containing 6 rats. 1st group was separated as a control group. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose to the 2nd group with i.p. way. BX was given to 3rd group 300mg/L/day dose with drinking water during 150 days. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose with i.p. way and BX were given with drinking water during 150 days to 4th group. At the end of the study blood analysis, tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) levels in 3-MC group; TOS and oxidative stress index (OSI), platelet (PLT) levels in 3-MC and 3-MC+BX groups showed significantly increases when compared to other groups. It was determined that lymphocytes % (LY%) of ever 3 groups were significantly higher; however, neutrophil % (NEU%) were significantly fewer according to control group. Haemoglobin (HGB) and hematocrit (HCT) values of 3-MC+BX groups showed significantly decrease according to other groups (P <= 0.05). Mean corpusculer volume (MCV) in 3-MC and 3-MC+BX groups showed significantly decrease when compared to other groups (P <= 0.05). As a result, in case of exposure to 3-MC, long-term use of BX with oral ways may not decrease oxidative stress, may changes haematolojical parameters such as, WBC, LY%, NEU%, PLT, HGB, HCT, MCV. However, these changes remain within physiological limits. Even so, in the use of BX should be considered use of iron. Furthermore, BX with the abovementioned dosage may be used to reduce the levels of TNF-alpha, IL-1 beta, IL-6 being inflammation and cancer markers.
Effects of Boric Acid on Proinflammation Cytokines, Total Oxidative-Antioxidative Status and Hematological Parameters in Rats Applied Benzo(A)pyrene
(Parlar Scientific Publications (p S P), 2020) Comba, Arzu; Oto, Gokhan; Comba, Bahat; Ozdemir, Hulya; Keskin, Siddik; Akveran, Gonul Arslan
The aim of this study was to evaluate effects of boric acid (BA) on tumor necrosis factor-alfa (TNF-alpha), interleukin 1 beta (IL-beta), interleukin-6 (IL-6), total oxidative (TOS)-total anti oxidative status (TAS) and leucocyte (WBC), erythrocyte (RBC), thrombocyte (PLT) and their other parameters in rats applied benzo(a)pyrene (B(a)P). In this study, rats were divided into 4 groups and each group were containing 6 rats. 1st group was separated as control group. 100 mg/kg total dosage of B(a)P was applied to the 2nd group 2 times a week total in 4 equal dosage with i.p. way. Only BA 300 mg/L was applied to 3rd group in drinking water every day during 150 days. B(a)P was applied twice a week first 2 weeks, 25 mg/kg dose with i.p. way and BA were given with drinking water during 150 days to 4th group. In the end of study was determined TNF-alpha and IL-1 beta levels in B(a)P group, increases compared with other groups. there was a statistically significant increase in levels of WBC and LY in group B(a)P+BA compared to other groups (P <= 0.05). % LY level in other groups was higher; its % NEU level was lower according to control group. RBC and PLT values in BA group were higher, than the other groups. HGB was higher in group BA than group B(a)P and BA+ B(a)P; HCT was higher in group BA than group B(a)P , significantly (P <= 0.05) As a result, neutropenia and lenfositos develop in case exposure of B(a)P and BA; furthermore, combination of B(a)P and BA increases WBC. BA used alone having considerable effect on excitation of eritopoezis and thrombocytosis. BA has protective effects for organisms together with decrease in the TNF-alpha and IL-1 beta levels caused by BA cure as solution of B(a)P exposition.
Effects of Cinnamaldehyde on Glucose-6 Dehydrogenase Activity, Some Biochemical and Hematological Parameters in Diabetic Rats
(Taylor & Francis Ltd, 2022) Celik, Remzi; Mert, Handan; Comba, Bahat; Mert, Nihat
Context Diabetes is a metabolic disorder related to blood insulin deficiency and high glucose level. Cinnamaldehyde is an important component of cinnamon and has an effect on blood glucose. Objective It was aimed to investigate the the effect of cinnamaldehyde on the liver glutathione (GSH), glucose-6-phosphate dehidrogenase (G6PD) activity, blood glucose, protein, lipid and erythrocyte parameters, live weight in diabetic rats. Material and Methods Rat used for this research were divided 4 group as control, diabetic, cinnamaldehyde and diabetic + cinnamaldehyde group. The live weight and fasting blood glucose level, taken from tail vein were recorded every ten days. End of the trail the blood samples were taken from rats. Biochemical parameters with autoanalyzer and hematological parameters with blood cell counter were determined in blood. The activity of G6PD and GSH amounts were measured with ELISA in the liver tissues. Results Blood sugar, triglyceride, total cholesterol, VLDL, LDL, and urea levels increased in diabetic rats, and cinnemaldehyde significantly decreased these parameters. Cinnemaldehyde also showed a positive effect on body weight, blood total protein, and mean corpuscular volume in diabetes. A decrease in HbA1c and an increase in liver G6PD, GSH activity were found in treatment group, but these changes were not statistically significant. Conclusion In conclusion, the antidiabetic, hypolipidemic and antioxidant effects of cinnamaldehyde were determined. It has also been shown to improve anaemia, urea levels and weight loss.
Experimental Study of In-Vitro Bioanalysis and In-Vivo Living Tissue Biocompatibility of Mg-Zn Alloys
(Springer Heidelberg, 2023) Comba, Bahat; Cicek, Bunyamin; Comba, Arzu; Sancak, Tunahan; Akveran, Gonul Arslan; Koc, Erkan; Afshar, Milad Torkamanian
In this study in-vitro bioanalysis and in-vivo living tissue biocompatibility were evaluated. Pure Mg and Mg + x Zn (x = 1 and 3 wt%) alloys have been used in the study. In vitro immersion and potentiodynamic corrosion were applied to these alloys in Hank's and artificial seawater solution. The materials were kept in living tissue (gluteus-superficial) for 7 weeks in-vivo (albino-rat). After 4 and 7 week, the control and experimental groups' urine parameters, blood mineral substance levels, and radiographic image controls were evaluated. It was observed that the bio-dissolution level decreased as the Zn-ratio increased. As a result, successful results were obtained in the biocompatible and biodegradable material class of Zn-doped Mg alloys used in the study. The dissolution amount of 1.03 mm/y (for Mg + 3Zn alloy) in Hank's solution was calculated. The % vitality was determined at the level of 117%.
Florozisin Koyunlarda Bazı Mineraller ve Hormonlar Üzerine Etkilerinin Araştırılması
(2016) Çınar, Ali; Comba, Bahat
Bu çalışmada; florozisin koyunlarda plazma flor (F), serum kalsiyum (Ca), fosfor (P), magnezyum (Mg), kalsitonin (CT), intakt paratiroid hormon (iPTH) ve vitamin D3 (Vit D3) üzerine etkileri araştırıldı. Araştırmada 3-4 yaşlarında 50 tanesi Tendürek Dağı (yaklaşık 2000 m) bölgesinde kronik florozisli (Florozisli grup) ve 25 tanesi Van bölgesinden (1700 m) florozis belirtisi göstermeyen (Kontrol grubu) toplamda 75 Morkaraman Koyunu kullanıldı. Türkiye' nin Doğu Anadolu bölgesindeki VanAğrı şehirlerinden kronik florozisli 50 koyundan toplanan veriler, Van bölgesinde florozis belirtisi göstermeyen 25 koyunla karşılaştırıldı. Plazma F seviyeleri spesifik flor elektrodu ile ölçüldü, serum Ca, P ve Mg seviyeleri fotometrik yolla, serum CT ve iPTH seviyeleri immünometrik kemilüminesans assay yöntemi ile belirlendi, serum Vit D3 seviyesi HPLC metodu ile analiz edildi. Florozisli gruptaki serum P ve Vit D3 seviyeleri düşük (p<0.001), serum CT (p<0.01) and iPTH (p<0.001) düzeyleri kontrol grubundan yüksekti. Ca ve Mg değerleri istatistiksel olarak önemsizdi. Elde edilen bulgular, uzun süreli ve yüksek miktarda flora maruz kalma sonucunda koyunlarda osteomalatik durumun oluşabileceğini desteklemektedir. Sonuç olarak, florozis şekillendikten sonra dişlerde ve iskelet sisteminde dönüşü olmayan patolojik bir durum söz konusu olduğundan, bu bölgede yaşayanlara temiz su kaynakları verilmeli. Aksi durumda florun dişler ve kemikler üzerindeki olumsuz etkisini önlemek için uygun dozlarda Ca, P, Mg ve Vit D3 ilavesi yapılmalıdır.
How Long-Term Intake of Sodium Fluoride (Naf) in Different Doses and 7,12 Dimetilbenz(A)antrasen (Dmba) Affect Erythrocyte Fragility and Parameters in Rats
(Wiley, 2017) Comba, Bahat; Oto, Gokhan; Arihan, Okan; Comba, Arzu; Uyar, Hasan
In Case of Obesity, Longevity-Related Mechanisms Lead To Anti-Inflammation
(Springer, 2014) Kaya, Mehmet Salih; Bayiroglu, Fahri; Mis, Leyla; Kilinc, Dide; Comba, Bahat
The exact mechanisms which contribute to longevity have not been figured out yet. Our aim was to find out a common way for prompting longevity by bringing together the well-known applications such as food restriction, exercise, and probiotic supplementing in an experimental obesity model. Experimental obesity was promoted in a total of 32 young (2 months old) and 32 aged (16 months old) male Wistar albino rats through 8-week cafeteria diet (salami, chocolate, chips, and biscuits). Old and young animals were divided into groups each consisting of eight animals and also divided into four subgroups as obese control, obese food restriction, obese probiotic-fed and obese exercise groups. Probiotic group diet contained 0.05 %w/total diet inactive and lyophilized Lactobacillus casei str. Shirota. The exercise group was subjected to treadmill running 1 h/day, at 21 m/min and at an uphill incline of 15 % for 5 days a week. Food restriction group was formed by giving 40 % less food than the others. The control group was fed regular pellet feed ad libitum. This program was continued for 16 weeks. Blood samples from all the groups were analyzed for fasting glucose, insulin, IGF-1, insulin-like growth factor binding protein 3 (IGFBP-3), interleukin (IL)-6, IL-12, malondialdehyde (MDA), fT3, TT3, fT4, TT4, and liver tissue MDA levels were measured. All applications showed anti-inflammatory effects through the observed changes in the levels of IGFBP-3, IL-6, and IL-12 in the young and old obese rats. While the interventions normally contribute to longevity by recruiting different action mechanisms, anti-inflammatory effect is the only mode of action for all the applications in the obesity model.
Investigation Effects on Some Mineral Matters and Hormones of Chronic Florozis in Sheep
(2010) Comba, Bahat; Çınar, Ali
Bu çalışmada; kronik florozisin koyunlarda plazma flor (F), serum kalsiyum (Ca), fosfor (P), magnezyum (Mg), kalsitonin (CT), parathormon (İPTH) ve vitamin D (vit D3) üzerine etkileri araştırıldı. Araştırmada hayvan materyali olarak 3?4 yaşlarında 50 adet Kronik Florozisli ve 25 adet Sağlıklı Morkaraman Koyunu kullanıldı. Kronik Florozisli ve Sağlıklı Koyunlar' ın plazma F seviyeleri spesifik flor elektrodu ile; serum Ca, P ve Mg ölçümü spektrofotometrik yolla; serum CT ve İPTH analizi enzim işaretli immünometrik kemilüminesans yöntemi ile; serum vitamin D3 ölçümü HPLC metodu ile belirlendi. Kronik Florozisli ve Sağlıklı Koyunlar' da plazma F düzeyleri 0.38±0.04, 0.13±0.02 ppm; serum Ca seviyeleri 9.34±0,37, 9.15±0.17 mg/dl; P miktarları 2.28±0.21, 6.23±0.32 mg/dl; Mg düzeyleri 2.51±0.34, 2.44±0.18 mg/dl; CT seviyeleri 192.28±15.35, 165.13±12.53 pg/ml; İPTH miktarları 16.20±1.39, 7.41±0.21 pg/ml ve vitamin D3 düzeyleri 0.023±0.004, 0.033±0.005 µg/ml olarak sırasıyla bulundu. Endemik florozisli bölgede uzun süre yaşayan insan ve hayvanlarda Ca, P, Mg ve vitamin D eksikliğine bağlı olarak sekonder hiperparatiroidizm gelişebilmektedir. Sonuç olarak, kronik florozis şekillendikten sonra dişlerde ve iskelet sisteminde dönüşü olmayan patolojik bir durum sözkonusu olduğundan, bu bölgede yaşayanlara temiz su kaynakları verilmeli, aksi durumda florun dişler ve kemikler üzerindeki olumsuz ekisini önlemek için uygun dozlarda Ca, P, Mg ve vitamin D ilavesi yapılmalıdır.
Investigation of In-Vitro Biocompatibility and In-Vivo Biodegradability of Am Series Mg Alloys
(Taylor & Francis Ltd, 2022) Comba, Arzu; Cicek, Bunyamin; Comba, Bahat; Sancak, Tunahan; Akveran, Gonul Arslan; Sun, Yavuz; Afshar, Milad Torkamanian
In this study, in vitro/vivo biocompatibility experiments of Mg-Al-Mn alloy were carried out, and AM50 and AM60 alloys were used in the experiments. Immersion and potentiodynamic corrosion have been applied to these alloys in vitro. For biocompatibility, cytotoxicity test was performed. A total of 40 rats were used for the in-vivo experiment. AM series alloys were surgically placed in the femoral condyle as mini screws and monitored radiographically. AM alloys were removed together with the femur at the end of 7 weeks and further examined. After in-vivo application, the biodegradability level was successfully concluded with blood mineral analysis and urinalysis. In this study, the bioprocess of AM series Mg alloys with Al and Mn additions has been completed. As a result, the AM alloys used can be classified as biocompatible and biodegradable materials.
Investigation of the Immunocastration Efficacy and Longevity of a Single Dose Gnrh Vaccine in Young Male Rats
(Parlar Scientific Publications (p S P), 2019) Eski, Funda; Mis, Leyla; Tasal, Ibrahim; Uslu, Baris Atalay; Comba, Bahat
Conventionally, immunization protocol require an initial priming immunization followed by an antigen boost for adequate immunity and long-term immunization. In this study we were evaluate the immunocastration efficacy and longevity of immunization with a single-dose GnRH vaccine (Repro-Bloc (TM)) in young male rats. Rats in the vaccine group (VAC, n: 35) were treated with GnRH vaccine as single-dose (200 mu l) and the control group (C, n: 15) were also injected with saline solution. Blood samples from all rats were taken monthly for 6 months and at 12th month. GnRH antibody titers, testosterone and leptin levels were determined by commercial RIA kits. Rats at 6th and 12th months were sacrificed for histologic analysis of testes. GnRH antibody levels were found similar in the VAC and C groups (P>0.05) at -1 week. As compared to the C, GnRH antibody levels (pg/ml) of the VAC group at 1st, 2nd, 3rd and 4th months rose continuously and reached the peak level at 5th month, decreased at 6th month and increased again by the 12th month (P<0.05), whereas in the C group GnRH antibodies weren't different (P>0.05). Compared to the unchanged testosterone levels within the process in C group, serum testosterone levels of the VAC group were lower at 2nd month and higher at 3th month, and thereafter lower (P<0.05). Leptin levels were no difference between groups (P>0.05). There were significant differences in the testicle weights, sperm motility, sperm numbers and abnormal sperm rates between VAC and C groups at 6th and 12th months (P< 0.05). Histological examination showed no significant difference in testes between groups at 6th and 12th months (P>0.05). In conclusion, although the vaccine reduced the germinative and endocrine testicular function from 6th months to 12th months with the single-dose GnRH vaccine, the single-dose vaccination did not provide adequate effect in the genital organs in male rats. Further studies are needed to determine an optimal single-dose, longevity of the vaccine and route of administration for complete suppression on the genital organs in different animal species.
An Investigation of the Protective Effects of Resveratrol on Some Biochemical Parameters and Histopathological Findings in Experimentally-Induced Chronic Flurosis in Rats
(int Soc Fluoride Research, 2017) Yildirim, Serkan; Oto, Gokhan; Comba, Bahat; Ekin, Suat; Cinar, D. Ali
This study was carried out to determine the protective effects of resveratrol on some biochemical parameters and histopathological findings in experimentally-induced chronic fluorosis in rats. Forty male Wistar albino rats, weighing 250-300 g, were randomly divided into four groups of 10 rats and treated for 12 weeks as follows: (i) control group (drinking water=tap water), (ii) resveratrol group (drinking water=50 mg resveratrol/L), (iii) NaF group (drinking water=10 mg NaF/L), and (iv) NaF+resveratrol group (drinking water=10 mg NaF + 50 mg resveratrol/L). The animals were sacrificed at the end of the 12 weeks. The hematological values of the control, resveratrol, NaF, and NaF+resveratrol groups were, respectively: RED BLOOD CELLS: 6.99 +/- 0.35, 6.77 +/- 0.41, 6.60 +/- 0.57, and 6.84 +/- 0.65 x10(6)/mm(3); HEMATOCRIT VALUE: 40.93 +/- 2.47, 43.54 +/- 3.38, 40.67 +/- 3.12, and 40.73 +/- 5.21 %; HEMOGLOBIN CONCENTRATION: 13.74 +/- 0.41, 14.62 +/- 0.37, 13.00 +/- 0.49, and 13.38 +/- 0.52 g/dL; PLATELETS: 369.85 +/- 65.73, 383.67 +/- 71.15, 291.25 +/- 66.51, and 351.83 +/- 71.23 x10(3)/mm(3); VITAMIN E: 1.042 +/- 0.045, 1.060 +/- 0.088, 0.977 +/- 0.070, and 0.998 +/- 0.060 mu mol/L; VITAMIN A: 3.141 +/- 0.107, 3.071 +/- 0.134, 2.555 +/- 0.093, and 2.90 +/- 0.131 mu mol/L; VITAMIN D: 0.653 +/- 0.043, 0.621 +/- 0.039, 0.419 +/- 0.039, and 0.582 +/- 0.046 mu mol/L, and VITAMIN K: 0.778 +/- 0.047, 0.756 +/- 0.068, 0.706 +/- 0.052, and 0.725 +/- 0.053 mu mol/L. The hematocrit hemoglobin levels in the resveratrol group were significantly increased (p<0.05) compared to the other groups. The platelet counts, vitamin A, and vitamin D levels were significantly lower (p<0.05) in the NaF group than in the other groups. The histopathological findings were: (i) in the control and resveratrol groups, the liver and bone tissue were found to have a normal histological structure, (ii) in the NaF group, hydropic degeneration and colangiohepatitis were detected in the liver, (iii) in the NaF+ resveratrol group, liver degeneration and colangiohepatitis were not found in the liver, (iv) in the NaF group, thinning of the bone tissue trabeculae and a significant decrease in the cellular density of the epiphyseal growth plate were observed, (v) in the NaF+ resveratrol group, a slight degree of thinning was detected in the bone trabeculae and the epiphyseal plate. It was concluded that resveratrol has protective effects on some biochemical parameters and histopathological findings in chronic fluorosis.
Is There a Relationship Between Serum Minerals (Ca, Mg) and Trace Elements (Cu, Fe, Mn, Zn) at Mating on Pregnancy Rates in Fat-Tailed Morkaraman Sheep
(Agricultural Research Communication Centre, 2017) Uslu, Baris Atalay; Mis, Leyla; Gulyuz, Fetih; Comba, Bahat; Ucar, Omer; Tasal, Ibrahim; Wehrend, Axel
The specific roles of nutrients in reproduction are not well defined in sheep. Hence, the relationships between certain mineral (Ca, Mg) and element (Cu, Fe, Mn, Zn) levels at mating and pregnancy rates was investigated in fat-tailed Morkaraman ewes synchronised with different hormones (melatonin, norgestomet implant and eCG) early in anoestrus season. For this purpose, 40 healthy ewes (aged 2-4 years) allocated in the synchronised (n=30) and no-treated group (n=10) were used during mid-May (suckling period). On the eCG injection day, fertile rams (n=8) were introduced into the groups and, then the oestrus signs were visually monitored twice daily for 7 days. The oestrous ewes were allowed to hand-mate using rotational (four rams daily) mating system. Additionally, jugular blood samples were collected on the days of onset of oestrus (before mating) for determination of serum Ca, Cu, Fe, Mg, Mn and Zn levels. Presence of pregnancies was diagnosed by real-time ultrasonography 35 days post-mating. Totally, 25 ewes became pregnant (21 ewes with stimulated-and 4 with spontaneous oestrus), while 9 ewes (all received stimulation) were not pregnant. In non-pregnant ewes, unlike other minerals and elements, the Cu and Zn values were significantly lower than their reference values (for Cu: 0.87 +/- 10.16 mg/dl vs. 9.20 mg/dl; for Zn: 2.06 +/- 10.30 mu g/dl vs. 3.8 mu g/dl, resp.; P<0.01 in both cases). In pregnant ewes, the corresponding values remained unchanged (P>0.05). Hence, the present findings indicate that the low Cu and Zn levels during the oestrus might adversely affect the subsequent pregnancy rate in suckling ewes.
Leptin Levels and Lipids Profile Determination in Different Sheep Breeds
(Univ Agriculture, Fac veterinary Science, 2016) Comba, Arzu; Mert, Handan; Comba, Bahat
Leptin hormone is produced by fat cells and is believed to coordinate the control of body weight. This study was conducted to determine the levels of leptin, triglyceride, cholesterol, VLDL, LDL, HDL and glucose in serum apart from the body weight of animals, and to find out the correlations between them. Four breeds of sheep (Karakul; semi-fat tailed breed, Morkaraman and Norduz; fat tailed breeds, Tahirova; thin tailed breed) were used as research material under the same feeding conditions. Fifteen sheep of each group -for a total of 60- were selected as research material. ELISA tests were used to determine serum leptin levels; auto analyzer was used for the estimation of triglyceride, cholesterol, VLDL, LDL, HDL and glucose levels. Body weights were measured by a weighing scale. Leptin levels, along with triglyceride, cholesterol, VLDL, LDL, HDL levels and body weight, were higher in Tahirova sheep breed, and the difference was found statistically significant (P<0.05). There was no statistically significant difference between the HDL levels of sheep breeds. Glucose levels were high in Karakul breed and statistically significant difference was determined between Karakul, Norduz and Tahirova breeds (P<0.05). In conclusion, thin tailed sheep breeds were found to have a high leptin level, which shows that leptin levels are independent of the tail fat ratios. Leptin has been studied in detail in human medicine, but researches on animals were scarce. Apparently, further studies are needed to elucidate the factors of production of leptin in animals. (C) 2015 PVJ. All rights reserved