Browsing by Author "Demir, Canan"

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3.5 Ghz Radiofrequency Radiation May Affect Biomechanics of Bone and Muscle of Diabetics
(Taylor & Francis Ltd, 2023) Bektas, Hava; Dasdag, Suleyman; Nalbant, Asrin; Akdag, Mahmut Berat; Demir, Canan; Kavak, Servet
With the developments in wireless technologies, living beings are increasingly exposed to electromagnetic fields (EMFs). EMFs are known to affect bone metabolism and muscle tissue. However, their effects on bones and skeletal muscles are controversial, as some studies have reported positive effects while others have reported adverse effects. In this study, the effects of radiofrequency radiation (RFR) on bone biomechanics and skeletal muscle tissues were investigated in diabetic and healthy rats. Rats were exposed to 3.5 GHz RFR for 2 h per day for 30 days. Bone biomechanics measurements were taken to evaluate the effects of RFR on bone quality, flexibility and durability. The whole-body specific absorption rate (SAR) was found to be 37 mW/kg. The results showed that RFR exposure had adverse effects on bone biomechanics, including decreased elasticity coefficient and Young's modulus, increased maximum displacement and decreased maximum force. However, oxidative stress parameters in diabetics were also altered by 3.5 GHz RFR to a greater extent than in healthy rats. In conclusion, 3.5 GHz RFR may have potential to alter bone quality and structural integrity including muscle oxidative stress parameters in rats. It should be emphasized that the observed changes were more obvious in diabetic rats. In addition, the changes observed in healthy and diabetic rats exposed to RFR showed a statistically significant difference according to the sham groups.
Adverse Effects of 900, 1800 and 2100 Mhz Radiofrequency Radiation Emitted From Mobile Phones on Bone and Skeletal Muscle
(Taylor & Francis inc, 2023) Bektas, Hava; Nalbant, Asrin; Akdag, Mahmut Berat; Demir, Canan; Kavak, Servet; Dasdag, Suleyman
The goal of this study was to biomechanically and morphologically research both the impact of mobile phone like radiofrequency radiations (RFR) on the tibia and the effects on skeletal muscle through oxidative stress parameters. Fifty-six rats (200-250 g) were put into groups: healthy sham (n = 7), healthy RFR (900, 1800, 2100 MHz) (n = 21), diabetic sham (n = 7) and diabetic RFR (900, 1800, 2100 MHz) (n = 21). Over a month, each group spent two hours/day in a Plexiglas carousel. The rats in the experimental group were exposed to RFR, but the sham groups were not. At the end of the experiment, the right tibia bones and skeletal muscle tissue were removed. The three-point bending test and radiological evaluations were performed on the bones, and CAT, GSH, MDA, and IMA in muscles were measured. There were differences in biomechanics properties and radiological evaluations between the groups (p < .05). In the measurements in the muscle tissues, significant differences were statistically found (p < .05). The average whole-body SAR values for GSM 900, 1800 and 2100 MHz were 0.026, 0.164, and 0.173 W/kg. RFRs emitted from mobile phone may cause adverse effects on tibia and skeletal muscle health, though further studies are needed.
Alopecia Areata Different View; Heavy Metals
(Wolters Kluwer Medknow Publications, 2019) Ozaydin-Yavuz, Goknur; Yavuz, Ibrahim Halil; Demir, Halit; Demir, Canan; Bilgili, Serap Gunes
Background: Alopecia areata (AA) is a noncicatricial alopecia affecting any hair-bearing area. Although AA is considered to be an autoimmune disease, oxidative stress has been shown to be an important factor in the etiology of AA. Trace elements are highly essential for humans since they form the building blocks of large molecules, function as the cofactors of enzymes, and have some key biological functions. The aim of this study was to investigate serum levels of heavy metals like iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), magnesium (Mg), cobalt (Co), cadmium (Cd), and lead (Pb) in patients with AA. Materials and Methods: The study included a patient group of 30 with AA and a control group of 31 healthy individuals. Serum levels of heavy metals were measured using atomic absorption spectrophotometry. Results: The 30 patients included 17 (56.7%) men and 13 (43.3%) women with a mean age of 33.8 (range, 19-48) years. Serum Zn and Mn levels were significantly lower and serum Cd, Fe, Mg, Pb, Co, and Cu levels were significantly higher in the patient group compared to that of the control group (P<0.05 for all). Conclusion: The results indicated that low levels of Zn and Mn are associated with AA while other metals were normal. So Zn supplementation may have some beneficial effect in AA while Fe prescription is fruitless.
Anom Approach for Statistical Evaluation of Some Antioxidant Enzyme Activities
(Frontiers Media Sa, 2022) Demir, Canan; Keskin, Siddik; Sen, Fatih
Free radicals are chemical molecules that are more reactive and have an unpaired electron. Free radicals formed inside the cell oxidize biomolecules, leading to cell death and tissue damage. Antioxidants are molecules that can stabilize or inactivate free radicals before they damage the cell. In this study; the availability of Malondialdehyde, Superoxide dismutase, Catalase and Reduced glutathione levels as markers for related diseases was evaluated by examining whether and in what range they may vary in some diseases. In study, nine groups consist of prostate cancer, cirrhosis, liver transplantation, chronic kidney damage, acute kidney injury, X-ray exposure, CT exposure, MR exposure and Osteonecrosis. Analysis of means is a method developed to compare group means with the overall mean and presents the results graphically in an easy-to-understand manner without the required for any post hoc test. In addition, related characteristics were categorized as "low and high" and Nonlinear Principal Component Analysis was conducted to visually present their relationship with related disease types in two-dimensional space. The upper and lower decision lines were found 3.123 and 2.794 mu mol/L, respectively for Malondialdehyde. Those with cirrhosis, chronic kidney disease, acute kidney disease and tomography exposure were included in the upper and lower decision lines. Those with prostate cancer, osteonecrosis, and X-ray exposure were above the upper decision line and are found higher than the overall mean. Those with lung transplantation and MR exposure appear to be below the lower decision line and lower than the overall mean. The present study provides the first comprehensive assessment of the availability of Malondialdehyde, Superoxide dismutase, Catalase and Reduced glutathione levels as markers for some related diseases. This study has shown that Analysis of means can be used as an alternative graphical procedure for multiple group comparisons with an overall mean in the studies regarding as biochemical characteristics and relating diseases. In addition, Nonlinear Principal Component Analysis can be useful aid for decision marker in some biochemical characteristics and related diseases.
Approach Artificial Neural Network for Nonlinear Principal Components Analysis
(2019) Demir, Canan; Keskin, Sıddık
Doğrusal Olmayan Temel Bileşenler Analizi (DOTBA), aralarında doğrusal veya doğrusal olmayan ilişki bulunan veri kümeleri için rakamsal ve görsel sonuçlar veren açıklayıcı bir boyut indirgeme yöntemidir. DOTBA'da, sürekli değişkenlerin yanı sıra, kategorik ve sıralı değişkenler de aynı anda analize dâhil edilebilir. Analizde gözlenen değişkenler arasındaki ilişkilerin doğrusal olduğu varsayımına gerek yoktur. Bu çalışmada DOTBA için yapay sinir ağları yaklaşımı açıklanarak uygulama yapılmıştır. Uygulamada veri seti olarak 422 hastaya ait 19 değişkenli hipotiroidi verisi kullanılmıştır. Yapay sinir ağlarında temel bileşenler analizi ile birlikte yapay sinir ağlarında doğrusal olmayan temel bileşenler analizi kullanılarak elde edilen sonuçlar, tablo ve grafikler halinde sunularak yorumlanmıştır. İlk iki temel bileşen, DOTBA' da toplam varyansın %95.65'ini açıklarken, TBA'da %90.08'ini açıklamıştır. Sonuç olarak, DOTBA'nın TBA'ya göre yüksek bir varyans açıklama oranı ile başarılı sonuçlar verdiği ve böylece ileriye dönük yapılacak tahminlerde kullanılabileceği vurgulanmıştır. Anahtar Kelimeler: Doğrusal Olmayan Temel BileĢenler Analizi, Hipotiroidi, Temel BileĢenler Analizi
Assessment of Heavy Metal and Trace Element Levels in Patients With Telogen Effluvium
(Wolters Kluwer Medknow Publications, 2018) Yavuz, Ibrahim Halil; Yavuz, Goknur Ozaydin; Bilgili, Serap Gunes; Demir, Halit; Demir, Canan
Background: Despite a multitude of studies, etiology of primary chronic telogen effluvium (TE) remains incompletely understood. Essential heavy metals are associated with beneficial effects in humans as well as in other living organisms. However, they may lead to toxic effects when the exposure exceeds the higher tolerable limits. We wanted to assess the heavy metal and trace element levels in patients with chronic TE. Materials and Methods: A total of 40 subjects with chronic TE were included in the study, and 30 healthy women served as control. General and dermatological examinations were taken up in all individuals. Those patients with positive hair pull test were evaluated with the help of a trichogram. The presence of >20% telogen hair as documented by trichogram was a requirement for the study inclusion. UNICAM-929 spectrophotometry device was used for determining serum trace element and heavy metal concentrations. Results: In spite of an absence of significant differences in terms of average Zn concentration, weight, or height between patients and controls, significant differences were noted for Cd, Fe, Mg, Mn, Pb, Co, and Cu (P<0.05). Conclusion: Our results suggest that heavy metals may play a causative role in the development of chronic TE. However, contrary to previous reports, zinc did not appear to play an important etiological role, while these patients had elevated serum iron levels.
Can We Reduce Oxidative Stress With Liver Transplantation
(Soc Medical Biochemists Serbia, 2021) Aydin, Mesut; Dirik, Yaren; Demir, Canan; Tolunay, Harun; Demir, Halit
Background: The aim of this study was to determine the levels of lipid peroxidation (MDA) and antioxidants such as reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) in the blood serum of patients with cirrhosis and liver transplantation. Methods: In this study, serum malondialdehyde acid (MDA) levels, superoxide dismutase (SOD), reduced glutathione (GSH), and catalase (CAT) activities were measured spectrophotometrically and compared to the results of the healthy control group. Results: SOD, CAT and GSH activities were significantly decreased in the patient groups compared to the healthy control group (p<0.05). MDA levels were significantly higher in the patient group compared to the healthy control group (p <0.05). Conclusions: In conclusion, this study demonstrated that oxidative stress may play an important role in the development of liver cirrhosis and in liver transplantation. This study is the first one to show how MDA, SOD, CAT and GSH levels change in liver cirrhosis and liver transplantation, while further studies are essential to investigate antioxidant enzymes and oxidative stress status in patients with cirrhosis and liver transplantation.
Change of Antioxidant Enzyme Activities, Some Metals and Lipid Peroxidation in Alzheimer's Disease
(Carbone Editore, 2016) Arslan, Ayse; Tuzun, Fatma Aykan; Tamer, Sibel; Demir, Halit; Aycan, Abdurrahman; Demir, Canan; Gonullu, Edip
Introduction: Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive cognitive regression and memory loss. It has been claimed that oxidative stress and factors such as metal accumulation in the brain play important roles in the etiopathogenesis of the disease. Material and methods: The subjects of this study consisted of 24 individuals with Alzheimer's disease and 15 healthy agematched controls. Blood samples were withdrawn from the patients and healthy controls, and the activities of antioxidant enzymes SOD (Superoxide Dismutase), GSH (Glutathion), GSHPx (Glutathion peroxidase), GST (Glutathion S-Transferase) and MDA (Malondialdehyde) levels were determined by Spectrometer. Some metals and heavy metals were measured by atomic absorption spectrometry (AAS). Results: Biochemical analyses showed a significant decrease of the main enzymatic antioxidant defences (SOD, GSH, GST and GSHPx) and increased production of lipid peroxidation marker (MDA) in the serum of AD patients, compared to age-matched control group (p< 0.001). Also the levels of Zn, Mg, and Mn was lower and Fe, Pb, and Cd was higher in the patient group, compared to the control group. Serum Cu and Co levels did not differ significantly between the patient and control groups (p> 0.001). Conclusion: These results supports the theory that in AD there is a defect in the antioxidant defense system, which may lead to oxidative damage. Also alterations in some trace metals and their related enzymes may play a role of etiopathogenesis in AD.
Change of the Levels of Trace Elements and Heavy Metals in Threatened Abortion
(Lippincott Williams & Wilkins, 2019) Turan, Kasim; Arslan, Ayse; Uckan, Kazim; Demir, Halit; Demir, Canan
Backround: This study aimed to investigate the impact of changes in the serum levels of trace elements and heavy metals in threatened abortion, which is a common complication in early pregnancy. Earlier studies have shown that changes in the serum levels of some trace elements and increased serum concentrations of heavy metals are associated with spontaneous abortion; however, this relationship has not been fully clarified. Methods: The patient group consisted of 45 pregnant women diagnosed with threatened abortion. There were 40 healthy nonpregnant women in the control group. Blood samples were obtained from the patients and the healthy controls, and the concentrations of serum trace elements and heavy metals were measured using atomic absorption spectrophotometry. Results: Statistically significant decreases were found in the serum zinc (Zn), iron (Fe), magnesium (Mg) and manganese (Mn) levels, and significant increases were found in the serum copper (Cu), cadmium (Cd) and lead (Pb) levels in cases of threatened abortion in comparison to the controls (p < 0.001). However, there was no difference between the groups in terms of the serum cobalt (Co) levels (p > 0.001). Conclusion: Changes in the balance of some essential trace elements (especially decreases in Fe and Zn) and elevated concentrations of some toxic heavy metals in the blood may be important diagnostic and prognostic parameters for threatened abortion
Deri Kanseri Hastalarının Serum Oksidatif Stres Parametrelerinin Değerlendirilmesi
(2018) Bilgili, Serap Güneş; Yavuz, İbrahim Halil; Yavuz, Göknur Özaydın; Demir, Halit; Demir, Canan
Giriş ve amaç: Deri kanserleri en sık görülen kanserlerden biridir. Bu kanserler non melanomve melanom olarak genellikle iki grup altında incelenir ve çoğu nonmelanomadır. Deri kanserlerinin insidansı ve mortalite oranları tüm dünyada giderek artmaktadır. Bu çalışmada hücre içiantioksidanlar olan superoksit dismutaz (SOD), katalaz (CAT), redükte glutatyon (GSH), glutatyonredüktaz (GR), glutatyon peroksidaz (GPx) gibi moleküller ile oksidatif stres belirteci olan malondialdehit (MDA) değerlerini deri kanseri hastalarında incelemek istedik.Materyal ve metod: Prospektif çalışma modifiye edilen Dünya Helsinki Deklarasyonu'na göreuygulandı. Bu çalışma üniversite hastanesinin dermatoloji departmanında yapıldı. Çalışmaya 30deri kanseri hastası ve 30 sağlıklı gönüllü alındı. Çalışmaya katılanların serumlarında hücre içiantioksidantlar ve malondialdehit seviyeleri değerlendirildiBulgular: Çalışma 33 erkek (%55), 27 kadın (%45) olmak üzere toplam 60 kişi ile gerçekleştirilmiştir. Çalışmaya katılan kişilerin hasta ve kontrol grubuna göre yaş ve cinsiyet değişkenlerikarşılaştırılmış aralarında anlamlı farklılık saptanmamıştır (p>0.05). GPx, GR, GSH, SOD, MDA,CAT değerlerinin, hasta ve kontrol grup ortalamaları arasındaki fark istatistik olarak önemli bulunmuştur (p<0.05).Sonuç: Bu çalışma deri kanseri hastalarında antioksidan serum düzeylerinin azaldığını ve oksidatif stres belirteci olan MDA’nın yüksek olduğunu göstermiştir.
Determination of Maternal Lipid Peroxidation and Antioxidant Activities in Term and Preterm Birth in Different Weeks
(Bayrakol Medical Publisher, 2021) Arslan, Ayse; Uckan, Kazurn; Turan, Kasurn; Demir, Halit; Demir, Canan
Aim: It has been suggested that oxidative stress may be associated with various pregnancy complications, including preterm birth (PTB). However, the rote of oxidative stress in preterm births and its effects on the pregnancy process are not conclusive. Material and Method: In this study, oxidative stress parameters were investigated in maternal blood samples who delivered at preterm and term. One hundred twelve mothers (<37 gestational weeks) diagnosed with preterm delivery were included in the study as a patient group. Also, sixty-four women who delivered at term as a control group were included in the study. Serum antioxidant enzymes (CAT (Catalase), SOD (Superoxide dismutase), GSHPx (Glutathione peroxidase), GSH (Glutathione) and MDA (Malondialdehyde) levels were determined spectrophotometrically. Results: Serum antioxidant activity was lower in the preterm groups than in the control group. Also, serum lipid peroxidation levels were higher in the preterm groups than the control group (p< 0.05). Discussion: Our findings show that women with preterm birth have higher levels of oxidative stress. These results suggest that oxidative stress is associated with preterm labor. However, It is still unclear whether oxidative stress is a cause or a result of preterm birth.
Determination of Oxidative Stress Level and Some Antioxidant Activities in Refractory Epilepsy Patients
(Nature Portfolio, 2024) Yilgor, Abdullah; Demir, Canan
The aim of this study was to determine the levels of superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and malondialdehyde (MDA) in patients with refractory epilepsy. Serum superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and malondialdehyde (MDA) levels were determined using the spectrophotometer method. Refractory epilepsy patients' serum superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and malondialdehyde (MDA) levels were statistically significant compared to the healthy control group (p < 0.05). In conclusion, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and malondialdehyde (MDA) levels may play an important role in the etiopathogenesis of refractory epilepsy. This study was the first to investigate some parameters in refractory epilepsy disease.
Determination of Oxidative Stress Levels and Some Antioxidant Enzyme Activities in Prostate Cancer
(Taylor & Francis Ltd, 2019) Amar, Safa Ali Ahmed; Eryilmaz, Recep; Demir, Halit; Aykan, Serdar; Demir, Canan
In this study, the antioxidant enzyme activities such as (SOD, GSH, and CAT) and malondialdehyde (MDA) level which is the end product of lipid peroxidation, were determined from the serum samples taken from patients diagnosed with prostate cancer Van Yuzuncu Yl University Medical Faculty of Educational Research and Training Hospital and stanbul Bagcilar Education Research Hospital. The SOD, GSH, and CAT activity of patient groups was found significantly lower than the healthy control group in patients with prostate cancer (p<.05). Serum MDA level is found significantly high when compared to control groups. MDA levels increased in patients that suffer prostate cancer disorder. Whereas, firstly antioxidant enzymes activity of SOD, GSH and CAT have been decreased in control groups. Thus, we concluded that the cause of development of prostate cancer may be the result of an imbalance between the antioxidants and oxidative stress. As a result, SOD, CAT, GSH, and MDA may play an important role in the etiopathogenesis of prostate cancer.
Determination of Some Antioxidant Activities and Oxidative Stress Leves in Patients With Lip and Oral Cavity Cancer
(2022) Belli, Şeyda; Ozkol, Halil; Demır, Halıt; Aslan, Ali; Mer, Ali Hussein; Demir, Canan
Squamous cell carcinomas constitute the majority of oral cavity tumors. Poor oral hygiene, smoking, chronic irritation and alcohol consumption are counted as etiological factors. In study, it was aimed to determine oxidative stress levels and some antioxidant activities such as superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT) in cancer of the lip and oral cavity. Malondialdehyde (MDA) levels, SOD, GSH and CAT activities were measured using the spectrophotometric method. The results showed that SOD, CAT and GSH levels decreased significantly in the patient group (p<0.05), while MDA levels increased significantly (p<0.05). Lack of antioxidants can increase the development of oral and lip cancers.
Determination of Some Trace Elements and Heavy Metal Levels (Cu, Mn, Mg, Fe, Zn, Co, Pb, and Cd) in Blood Serum of Patients With Lip and Oral Cavity Cancers
(Polish Society Magnesium Research, 2023) Demir, Derya Cay; Demir, Halit; Bozan, Nazim; Belli, Seyda; Demir, Canan
This study investigated copper (Cu), manganese (Mn), magnesium (Mg), iron (Fe), zinc (Zn), cobalt (Co), lead (Pb) and cadmium (Cd) levels in the blood serum of patients with lip and oral cavity cancer. Our study aims to determine the relationship of some trace elements and heavy metals with lip and oral cavity cancer. Blood serum results of 21 individuals with lip and oral cavity cancer were compared with those in the control group consisting of 30 volunteer and healthy subjects. The serum levels of trace elements and heavy metals (Cu, Mn, Mg, Fe, Zn, Co, Pb and Cd) were determined by atomic absorption spectrophotometry at Yuzuncu Yil University Central Research Laboratory (Spectrometer: Thermo Scientific C103500100, China). In this study, when the descriptive statistics and comparison results for Cu, Mn, Mg, Fe, Zn, Co, Pb, and Cd were examined, the difference between the patient and healthy group means was found to be statistically significant (p<0.05). According to these results, Zn, Fe, Mn, Mg, and Cu levels which are essential trace elements for the human body, were significantly lower in patients than in healthy groups. On the other hand, Cd, Co, and Pb levels which are harmful and toxic to the human body were significantly higher in patients than in healthy persons (p<0.05). In conclusion, deficiencies of some trace elements, such as Cu, Mn, Fe, Zn, and Co, were detected in the blood serums of patients with lip and oral cavity cancer. Deficiencies of trace elements in patients with lip and oral cavity cancer may result from excessive nutrient con-sumption or malnutrition of cancer patients, in addition to which trace element and heavy metal levels (Cu, Mn, Mg, Fe, Zn, Co, Pb and Cd) can affect the etiopathogenesis of the disease.
The Effect of an Improved Environment According To Watson's Theory of Human Care on Sleep, Anxiety, and Depression in Patients Undergoing Open Heart Surgery: a Randomized Controlled Trial
(Mdpi, 2025) Azizoglu, Hatice; Gurkan, Zeynep; Bozkurt, Yasemin; Demir, Canan; Akaltun, Hatice
Background/Objectives: According to Watson's Human Care Theory, an improved environment influences patients' care processes. The purpose of this study was to examine the effect of an improved environment, according to Watson's Human Care Theory, on sleep quality, anxiety, and depression in patients undergoing open heart surgery. Methods: Upon admission to the ward from the postoperative intensive care unit, the experimental group underwent environmental remediation for three days. The environmental arrangements ensured that the patient's room maintained an appropriate temperature range of 18-26 degrees C and humidity values of 30-50%. Monitoring took place at 21:00, 22:00, and 23:00 on Days 1, 2, and 3, at which times, the brightness of the patient rooms gradually decreased. On the morning of the fourth day, the patients were interviewed face to face, and research questionnaires were filled out (ClinicalTrials.gov identifier of the manuscript: NCT06744023). Results: After the implementation of an improved environment in accordance with Watson's Human Care Theory, the sleep duration of the patients in the experimental group (5.91 h) was higher than that of the control group (4.1 h). At the same time, the mean sleep quality score was measured as 300 +/- 15.33 in the experimental group and 116.33 +/- 14.94 in the control group. In addition, anxiety and depression levels were lower in the experimental group (5.63 +/- 0.59; 4.53 +/- 0.42) compared with the control group (12.03 +/- 0.85; 10.03 +/- 0.82). Conclusions: We recommend implementing improved environmental arrangements in accordance with Watson's Human Care Theory to improve sleep quality and reduce anxiety and depression levels in patients undergoing open heart surgery.
The Effect of Computed Tomography on Oxidative Stress Level and Some Antioxidant Parameters
(Sage Publications Ltd, 2021) Gunduz, Ali Mahir; Demir, Halit; Toprak, Nursen; Akdeniz, Huseyin; Demir, Canan; Arslan, Ayse; Goya, Cemil
Background X-rays are defined as ionizing radiation and hydrolyze the water, causing free radical formation. Oxidative stress is the damage that occurs in cells due to the lack of antioxidants, which detoxifies them, with the increased production of free radicals that occur during normal cellular metabolism. Purpose To examine the acute effects of computed tomography (CT), i.e. ionizing radiation, on oxidative stress and antioxidant defense mechanisms. Material and Methods The study included a total of 53 patients that were selected among the patients that underwent non-contrast full-body CT. Malondialdehyde (MDA) and reduced glutathione (GSH) levels and superoxide dismutase (SOD) and catalase (CAT) activities were investigated in blood samples taken from patients. Results The post-scan levels of MDA increased significantly while the post-scan levels of GSH, SOD, and CAT decreased significantly compared to their pre-scan levels. Conclusion CT, which is a widely used X-ray imaging technique and has numerous known side effects, was found to increase the levels of MDA, which is an indicator of oxidative stress, and to decrease the levels of some antioxidants including GSH, SOD, and CAT.
The Effect of Local Anaesthetic Agents on Opioid Use and Recovery in Patients Undergoing Open Heart Surgery: a Randomized Controlled Study
(Bmc, 2025) Azizoglu, Hatice; Asam, Mehmet; Gurkan, Zeynep; Bozkurt, Yasemin; Demir, Canan
BackgroundAfter open heart surgery, patients experience high levels of pain at the sternotomy incision site and around the chest tube(s), affecting their recovery. This study was conducted to determine the effects of local anesthetic application around the sternotomy incision site and chest tube(s) on the amount of opioids used and recovery after surgery in patients undergoing open heart surgery.MethodsThis randomized controlled experimental study was conducted with a total of 60 patients, with 30 patients in the experimental group and 30 patients in the control group. In the experimental group, LIDOFAST 40 mg/2 ml + 0.025 mg/2 ml, a local anesthetic agent, was applied to the postoperative sternotomy incision site and around the chest tube(s) in addition to routine treatment. Patients in the control group received only routine treatment. Data were collected using the "Descriptive Characteristics Form" and the "Postoperative Recovery Index".ResultsIt was observed that postoperative pain started later, opioid consumption decreased, and postoperative recovery was faster in the experimental group. As the number of chest tubes increased, recovery was delayed in all groups, and as the number of opioids used increased, postoperative recovery was negatively affected in the control group.ConclusionsIn this study, local anesthetic application to the sternotomy incision site and around the chest tubes after open heart surgery was found to reduce postoperative opioid consumption and positively affect recovery.Trial registrationCurrent Controlled Trials NCT06642077 (Retrospectively registered).
Erythrocyte Catalase and Carbonic Anhydrase Activities in Lung Cancer
(Asian Pacific Organization Cancer Prevention, 2010) Cobanoglu, Ufuk; Demir, Halit; Duran, Memet; Sehitogullari, Abidin; Mergan, Duygu; Demir, Canan
Aim: To study the relationship between the pathogenesis of lung cancer and antioxidant status and acidic media by measuring the activities of erythrocyte catalase (CAT) and carbonic anhydrase (CA). Methods: A total of 26 patients with lung cancer and 15 healthy individuals were included in the study. The CAT and CA activities of erythrocytes were defined. The catalase (CAT) activity of erythrocytes was measured using Aebi's method. Carbonic anhydrase (CA) activity was analyzed by CO2 hydration. Results: It was found that erythrocyte CA and CAT activities were significantly lower in patients with lung cancer compared to controls (p<0.05). Of the 26 patients with lung cancer, seven (26.9%) had metastasis, and the CA and CAT levels in patients with metastasis were significantly decreased (p=0,0001). Conclusions: Development of oxidative stress due to lung cancer may be related to the balance between prooxidant and antioxidant reactions. Catalase may have a preventive effect for malignant lung cancers and the gene of the antioxidant enzymes may be one of the anti-oncogenes, and inactivation of one of these genes in the process of carcinogenesis may lead to tumor development. This may be an explanation for the very low levels of antioxidant CAT in patients with lung cancer compared to healthy individuals. Carbonic anhydrase (CA) in tumor cells may be an indicator of the acid-base balance in lung cancer. Decreased levels of CA in patients with lung cancer may provide a convenient media for tumor development, growth and metastasis by creating an acidic media.
Evaluation of Oxidative Stress in Angiography Workers
(2020) Demir, Canan; Gündüz, Ali Mahir
Aim: In this study, we wanted to investigate the oxidative stress level and some antioxidant parameters in angiography workers exposed to high dose x-ray.Material and Methods: The study included 47 angiography workers and 50 healthy control group. Some antioxidant parameters (superoxide dismutase, catalase, and reduced glutathione) and levels of malondialdehyde, which is a marker of oxidative stress, were measured in the blood samples of all participants.Results: There was a significant increase in oxidative stress marker MDA levels and a significant decrease in antioxidant enzyme (superoxide dismutase, catalase and reduced glutathione) levels in all individuals of the study group compared to the control group (p<0.001 for all).Conclusion: Our results indicated that oxidative stress level increased and some antioxidant enzyme levels (superoxide dismutase, catalase and decreased glutathione) decreased in angiography workers exposed to long-term high-dose ionized radiation. We have linked this to the oxidant-antioxidant balance disorder caused by increased free radicals due to ionizing radiation, as noted in theliterature.