Browsing by Author "Koyuncu, Ismail"

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Ameliorative Influence of Urtica Dioica L Against Cisplatin-Induced Toxicity in Mice Bearing Ehrlich Ascites Carcinoma
(Taylor & Francis Ltd, 2012) Ozkol, Halil; Musa, Davut; Tuluce, Yasin; Koyuncu, Ismail
Cisplatin (CP) is a widely used cytotoxic agent against cancer, and high doses of CP have been known to cause nephrotoxicity and hepatotoxicity. Some reports claim that antioxidants can reduce CP-induced toxicity. This study investigated the hepatoprotective, nephroprotective, and antioxidant activity of Urtica dioica L methanolic extract (UDME) against CP toxicity in Erhlich ascites tumor (EAT)-bearing mice. Levels of serum hepatic enzymes, renal function markers, and oxidant/antioxidant parameters of liver tissue were measured. Mice were inoculated with EAT on day 0 and treated with nothing else for 24 hours. After a single dose of CP administration on day 1, the extract was given at the doses of 50, 100, 200, and 400 mg/kg body weight daily during 6 days. Almost all doses of UDME performed a significant (P<0.05) preventive role against CP toxicity by decreasing aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, blood urea nitrogen, creatinine, lipid peroxidation, protein oxidation levels, and myeloperoxidase activity, as well as increasing reduced glutathione content, superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase activities. This suggests that UDME has a protective capacity and antioxidant activity against CP toxicity in EAT-bearing mice, probably by promoting antioxidative defense systems.
Anthocyanin-Rich Extract From Hibiscus Sabdariffa Calyx Counteracts Uvc-Caused Impairments in Rats
(Taylor & Francis Ltd, 2015) Ozkol, Hatice Uce; Koyuncu, Ismail; Tuluce, Yasin; Dilsiz, Nihat; Soral, Sinan; Ozkol, Halil
Context: Ultraviolet radiation (UV) was reported to cause oxidative stress. Hibiscus sabdariffa L. (Malvaceae) calyx is commonly used in traditional Asian and African medicines and possesses strong antioxidant capacity due to its anthocyanin (ANTH) content. Objective: This study researched the possible protective role of Hibiscus sabdariffa calyx extract (HSCE) in UVC exposure of rats. Material and methods: Levels of serum enzymes, renal function tests, and some oxidant/antioxidant biomarkers of skin, lens, and retina tissues were monitored. Rats were exposed to UVC 4 h daily for 40 d and simultaneously received HSCE containing 2.5, 5, and 10 mg doses of ANTH in drinking water. Results: Significant (p < 0.05) increases in the levels of serum aminotransferases, lactate dehydrogenase, urea, creatinine, and uric acid were noted after UVC exposure. In skin, lens, and retina tissues, total oxidant status, oxidative stress index, lipid peroxidation, and protein oxidation escalated markedly (p < 0.05) whereas total antioxidant status, reduced glutathione, and superoxide dismutase decreased dramatically (p < 0.05) related to UVC. Co-administration of HSCE with each ANTH dose significantly (p < 0.05) reversed aforementioned parameters (except total oxidant status) almost in all tissues. The LD50 of HSCE in rats was determined to be above 5000 mg/kg. Discussion and conclusion: Our data revealed that HSCE has a remarkable potential to counteract UVC-caused impairments, probably through its antioxidant and free radical-defusing effects. Therefore, HSCE could be useful against some cutaneous and ocular diseases in which UV and oxidative stress have a role in the etiopathogenesis.
Antiproliferative and Apoptotic Role of Novel Synthesized Cu(Ii) Complex With 3-(3 Benzenesulfonamide in Common Cancer Models
(int inst Anticancer Research, 2018) Tuluce, Yasin; Gorgisen, Gokhan; Gulacar, Ismail Musab; Koyuncu, Ismail; Durgun, Mustafa; Akocak, Suleyman; Kaya, Zehra
Background/Aim: Chemotherapeutic treatment options are often ineffective due to the development of resistance in cancer cells. Therefore, developing new anti-cancer agents is crucial for cancer treatment. Some triazine derivatives, their complexes and Copper(II) have anti-cancer effects on cancer cells. In this study, we aimed to determine the anti-proliferative effect of the novel synthesized Cu(II) complex with 3-(3-(4-fluorophenyl)triaz-1 -en- 1-yl) benzene-sulfonamide compound on the common cancer cell lines HeLa, MDA-MB-231, A2780 and MCF7. Materials and Methods: Common cancers cell lines were treated with copper complexes. Cell viability and apoptotic gene expression were examined. Results: Novel synthesized copper complex led to decreased viability of all cell lines. It also induced apoptosis via increasing the expression of caspase-3, caspase-9, Bax and p53 proteins and decreasing ERK expression. Conclusion: The novel synthesized copper complex has a significant inhibitory effect on the viability of cancer cell lines and can be considered as an antitumor agent for further studies.
The Apoptotic, Cytotoxic and Genotoxic Effect of Novel Binuclear Boron-Fluoride Complex on Endometrial Cancer
(Springer, 2017) Tuluce, Yasin; Lak, Pawan Tareq Ahmed; Koyuncu, Ismail; Kilic, Ahmet; Durgun, Mustafa; Ozkol, Halil
Endometrial cancer (EC) is one of the most common types of gynecologic cancer of the female genital tract; it considered being the fourth leading death factor among other types of cancer. Therefore, developing new anti-cancer agents are crucial for cancer treatment. Based on the potential of Schiff based complexes for the induction of apoptosis, Schiff base compounds, and their metal complexes displayed excellent anticancer properties. In this current study, antiproliferative activity of [L(BF2)(2)] as a novel binuclear boron-fluoride complex was examined to preliminary research in eight different cell lines, HELA, DU-145, PC3, DLD-1, ECC-1, PNT1-A, HT-29, and MCF-7, it was found to have a potent, suppressive effect on human endometrial adenocarcinoma cell line ECC-1. Based on this data, later investigated its apoptotic, cytotoxic, and genotoxic properties on human endometrial adenocarcinoma cell line ECC-1 in different concentrations. Apoptotic and cytotoxic tests such as single cell gel electrophoresis assay (comet assay), DNA fragmentation laddering, acridine orange test for DNA damage, and ELISA for apoptotic measurement was performed. We also gauged the oxidative status by evaluating total antioxidant status (TAS) and total oxidant status (TOS). Oxidative stress index (OSI) was calculated too. As a result [L(BF2)(2)] has been found to have a marvelous effect on ECC-1 cells, especially in damaging their DNA and cause a series of reactions lead to apoptosis. Taken together, it suggests that the [L(BF2)(2)] complex can induce the apoptotic pathway of endometrial cancer cells and is a possible candidate for future cancer treatment studies.
Attenuation of Uvc-Induced Toxicity by Linum Usitatissimum L Oil in Rats: Monitoring of Some Hematological and Biochemical Constituents
(Parlar Scientific Publications (p S P), 2011) Ozkol, Halil; Tuluce, Yasin; Koyuncu, Ismail
The aim of this study was to investigate the protective effect of Linum usitatissimum L. seed oil (LUSO) against ultraviolet C (UVC) exposure through monitoring some hematological and biochemical constituents of rats. For this purpose, rats were divided into three groups: control, UVC and UVC+LUSO. The rats of UVC and UVC+LUSO groups were subjected to UVC during 1 h twice a day for 4 weeks. LUSO (4 ml/kg b.w.) was given to rats of UVC+LUSO group by gavage before each irradiation period. Results showed that lipase and cholesterol levels were markedly higher in UVC group than control. Lipase decreased significantly, and cholesterol diminished slightly with combined treatment of UVC and LUSO. While the level of red cell distribution width (RDWc) decreased in UVC group, it increased significantly after LUSO supplementation. Apart from that, calcium and monocyte levels increased markedly in UVC+LUSO group compared with control and UVC groups. Significant decreases were determined in amounts of white blood corpuscles and lymphocytes as well as an increase was observed in monocyte percentage of UVC+LUSO group compared to control. This investigation showed that treatment of LUSO with UVC supplied relative protection against UVC injury and perfected some blood parameters significantly, even when compared with control group.
A Biochemical Study on the Protective Potential of Centaurium Erythraea L. in Rats at Acute Aspirin Exposure
(Parlar Scientific Publications (p S P), 2011) Ozkol, Halil; Tuluce, Yasin; Koyuncu, Ismail; Balahoroglu, Ragip
The aim of this study was to determine protective effect of Centaurium erythraea L. on rats exposed to aspirin (ASA). The protective influence of the 50% aqueous-ethanolic Centaurium erythraea L. extract (AECE) (100 mg/kg body weight) was investigated in rats exposed to a single dose of 200 mg/kg body weight ASA administration. Twenty-one Sprague-Dawley albino rats were distributed into three groups equally (n=7) as follows: A (control), B (only ASA-treated) and C (ASA+AECE-treated) group. At the end of 4 h ASA administration to groups B and C, some serum biochemical constituents were assigned and compared between the groups. Results showed that levels of aspartate aminotransferase, alanine aminotransferase, creatinine and potassium increased in group B compared with the control group, but they were decreased significantly with AECE supplementation. While lipase activity decreased only in group B, level of uric acid decreased both in groups B and C compared with control group. In conclusion, the current trial has shown that treatment of AECE with ASA supplied relative protection against acute ASA toxicity through ameliorating some blood parameters.
Calendula Officinalis L. Ameliorates Levels of Some Blood Constituents and Body Weight Loss in Passive Cigarette Smoke Exposure of Rats
(Parlar Scientific Publications (p S P), 2011) Ozkol, Halil; Tuluce, Yasin; Koyuncu, Ismail
The aim of this study was to investigate the protective effect of Calendula officinalis L. (CO) on rats exposed to cigarette smoke (CS). For this purpose twenty one albino male Sprague Dawley rats were randomly divided into three groups as control, CS and CS+CO. The rats of the CS and CS+CO groups were subjected to CS during 1 h twice a day for 23 days. CO (100 mg/kg body weight) was given to the rats of the CS+CO group by oral gavage one hour before each administration period. Protective effect of CO was detected by comparing some serum biochemical parameters and mean body weight changes of groups. While aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CK) levels of CS group increased, amylase and lipase decreased significantly compared to control. AST, LDH and CK levels reversed to nearly their control group values by CO supplementation. There was a significant reduction in the HDL level of the CS group compared to control. But its level increased markedly in the CS+CO group. With regard to calcium, its amount escalated significantly in the CS and CS+CO groups compared with control. Administration of CO caused an amendment in the body weight loss. In conclusion, this study revealed that CO could relatively combat the hazardous effects of CS and thus may help to reduce development risk of some disorders related to CS.
The Cytotoxic, Apoptotic and Oxidative Effects of Carbonic Anhydrase Ix Inhibitor on Colorectal Cancer Cells
(Springer/plenum Publishers, 2018) Tuluce, Yasin; Ahmed, Bewar Ali; Koyuncu, Ismail; Durgun, Mustafa
Colorectal cancer (CRC) is the third most common tumor, malignant and has developed one of the main reasons of cancer mortality. According to studies conducted recently; carbonic anhydrase 9 (CAIX) is an especially attractive target for cancer therapy, in part since it is limited way expressed in normal tissues on the other hand in a wide variety of solid neoplasia are overexpressed. The aim of this study was to appreciate the effects of CAIX inhibitor, namely novel synthesized sulfonamide derivative (H-4i) with high affinity for CAIX, in CAIX-positive human colorectal cancer cell (HT-29) and CAIX-negative human normal embryonic kidney cell line (HEK-293). For this reason, we planned to investigate apoptotic, cytotoxic and oxidative stress activity of H-4i on HT-29 and HEK-293 cell lines. Cell viability determined by WST-1 assay afterwards IC50 values, apoptosis and cell cycle induction measured by flow cytometric analysis, intracellular free radical induction performed by reactive oxygen species (ROS) analyses. The IC50 value of the sulfonamide derivative compound was found to be very low, especially in HT-29 cells, when compared to human normal cells. This research found that H-4i significantly increased cytotoxicity and ROS production, caused significant signs of apoptosis level. High level of ROS and apoptosis lead to arrest the cell cycle and reduce cell survival. The most obvious finding to emerge from the analysis that novel synthesized sulfonamide derivative H-4i is effective on HT-29 more than HEK-293. Therefore, novel derivative H-4i might be used as an anti-cancer potential compound on CRC.
Determination of Serum Differential Carnitine Ester Levels in Hiv(+)patients: a Cross-Sectional Study
(Bentham Science Publ Ltd, 2023) Binici, Irfan; Akbay, Halil Ibrahim; Huyut, Zubeyir; Alp, Hamit Hakan; Karsen, Hasan; Koyuncu, Ismail; Celik, Hakim
Objective It has been reported that carnitine deficiency is observed in various viral infections and in the follow-up of the prognosis of some diseases. In this cross-sectional study, we aimed to determine how carnitine ester derivatives change in HIV-positive patients. Materials and Methods In this study, 25 HIV-infected patients who applied to Harran University Faculty of Medicine Education Research and Practice Hospital Infectious Diseases and Clinical Microbiology Outpatient Clinic and who did not receive any antiretroviral treatment, as well as 25 healthy volunteers were included in the study. Carnitine ester levels in serum samples were measured by Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) method (Shimadzu North America, Columbia, MD, USA). Results While suberoylcarnitine (C8DC), myristoleylcarnitine (C14:1), tetradecadienoylcarnitine (C14:2), palmitoleylcarnitine (C16:1), and linoleylcarnitine (C18:2) levels in HIV(+) patients were quite low compared to the control group, tiglylcarnitine (C5:1) levels were high (p <= 0.05). In addition, C5:1 and C14:2 index parameters according to VIP score, and C5:1 and C14:1/C16 index parameters according to ROC analysis were determined as markers with high potential to distinguish HIV(+) patients from healthy volunteers. Conclusion This study showed that levels of acylcarnitine derivatives might be altered in HIV(+) patients, and the results obtained may contribute to a better understanding of carnitine metabolism.
The Effect of a Bis-Structured Schiff Base on Apoptosis, Cytotoxicity, and Dna Damage of Breast Cancer Cells
(Wiley, 2022) Tuluce, Yasin; Hussein, Azhee Ibrahim; Koyuncu, Ismail; Kilic, Ahmet; Durgun, Mustafa
Developing new anticancer agents are crucial for cancer treatment. Antiproliferative activity of L1H as a bis-structured Schiff base was subjected to preliminary research in eight different kinds of cell lines by the cell viability method using different concentrations to determine their inhibitory concentration. L1H demonstrated the highest cytotoxicity in human breast cancer cell line MCF-7. In this perspective, the MCF-7 cell line was cultured for the examination of different molecular techniques, including MTT, apoptosis analysis by enzyme-linked immunosorbent assay (ELISA), and comet assay. Moreover, the DNA ladder, acridine orange/ethidium bromide as another apoptotic cell analysis, markers of oxidative stress, and total antioxidant status, total thiol, and GSH as nonenzymatic antioxidants assay were conducted. The above techniques have proven that L1H is a growth inhibitor effect when compared to cisplatin as a positive control in human breast cancer cells, especially those affected by L1H. The findings clearly show that L1H evaluated in MCF-7 cell lines causes rising or induced apoptosis, DNA damage, diminished antioxidant status against the increase of oxidized protein, and prevents cell proliferation. Manifold evidence supported our hypothesis that L1H has a potential therapeutically improved effect against the MCF-7 cell line, and then without a doubt is a suitable candidate drug for investigating cancers next.
Evaluation of the Anticancer Potential of a Sulphonamide Carbonic Anhydrase Ix Inhibitor on Cervical Cancer Cells
(Taylor & Francis Ltd, 2019) Koyuncu, Ismail; Tuluce, Yasin; Qadir, Hewa Slahaddin; Durgun, Mustafa; Supuran, Claudiu T.
Cervical cancer is a common type of cancer. Carbonic anhydrase IX (CA IX) is an attractive target for tumour therapy, being overexpressed in many cancers. We investigated the anticancer properties of the aromatic sulphonamide S-1 as a CA IX inhibitor on cervical cancer cells (HeLa) positive for CA IX expression and normal prostate epithelial cell line (PNT1-A) negative for CA IX. We examined the cytotoxic, apoptosis, genotoxic, and oxidative stress activity of S-1 on HeLa and PNT1-A cell lines. S-1 induced significant reduction of cell viability, caused apoptosis, and up-regulated ROS production. This decrease in cell survival rate can be attributed to the high level of ROS and apoptosis, which has also been shown to arrest the cell cycle. Our findings indicated that S-1 is more effective on HeLa than PNT1-A. S-1 was able to induce apoptosis of cervical cancer cells and is a possible candidate for future anticancer studies.
Gastroprotective Effect of Small Centaury (Centaurium Erythraea L) on Aspirin-Induced Gastric Damage in Rats
(Sage Publications inc, 2011) Tuluce, Yasin; Ozkol, Halil; Koyuncu, Ismail; Ine, Hatice
The aim of this study was to determine the antiulcer and antioxidant activities of Centaurium erythraea L (small centaury) in aspirin (ASA) induced acute gastric ulcer model. The gastroprotective effect of the 50% aqueousethanolic small centaury (SC) extract was investigated in rats at a dose of ASA 200 mg/kg body weight. Twentyone Sprague-Dawley albino rats were divided into three groups of seven rats each as follows: (1) control group; (2) acute ASA-treated group and (3) ASA plus SC group. At the end of the 4-h drug administration, ulcer index, oxidant and antioxidant levels were measured and compared between the groups. The percentage of lesion area to total gastric surface area (ulcer index) was significantly reduced (77%) in ASA plus SC group as compared with acute ASA-treated group. The oral administration of ASA decreased catalase (CAT), reduced glutathione (GSH), and increased lipid peroxidation (LPO) levels. Although myeloperoxidase (MPO) activity was increased by ASA, it was found to be lower in the ASA plus SC group. GSH and Vitamin A levels were determined higher in the ASA plus SC group compared with ASA group. These results suggest that SC extract protects against ASA-induced damage due to its antioxidizing activity.
Increased Occupational Coal Dust Toxicity in Blood of Central Heating System Workers
(Sage Publications inc, 2011) Tuluce, Yasin; Ozkol, Halil; Koyuncu, Ismail; Ine, Hatice
Coal dust causes lung diseases in occupational exposure. Reactive oxygen species have been implicated in the pathogenesis of its toxicity. In this study, serum enzymes, lipid profile and other biochemical values with oxidant/antioxidant status in whole blood and serum of central heating system workers (CHSW; the persons responsible for heating the apartment with coal) were determined to reflect the cell injury. Blood samples were obtained from CHSW (n = 25) and healthy individuals (n = 25). All values were measured in whole blood and serum. ANOVA was used for the estimation of statistical data. In the group of CHSW, creatinine, ferritin, alanin aminotransferase, aspartate aminotransferase, creatine phosphokinase, gamma glutamyl transferase, lactate dehydrogenase and glutathione reductase activities as well as triglyceride, very low density lipoprotein, protein carbonyl and malondialdehide were significantly higher, while transferrin, high density lipoprotein and catalase (CAT) activities were lower than the group of healthy individuals. This result is consistent with hypothesis that respirable coal dust generates lipid and protein oxidation and induces leakage of serum enzymes by cell damage. It also leads to imbalance in antioxidant defense system, lipid profile and other biochemical parameters.
Novel Fluorine Boron Hybrid Complex as Potential Antiproliferative Drugs on Colorectal Cancer Cell Line
(Bentham Science Publ Ltd, 2019) Tuluce, Yasin; Masseh, Hawro D. I.; Koyuncu, Ismail; Kilic, Ahmet; Durgun, Mustafa; Ozkol, Halil
Objective: Colorectal Cancer (CRC) is one of the most common types of cancer in both sexes; it is considered to be the third leading death factor among other types of cancer. This study aimed to examine the cytotoxicity of a new fluorine boron hybrid complex [L(BF2)(2)] on human colorectal adenocarcinoma cell line (HT-29), based on the potency of the half-metal based complexes to initiate apoptosis. Methods: Based on this data, the impact of it in different concentrations on HT-29 cancerous cells was determined by apoptosis (ELISA, DNA fragmentation laddering, AO/EB staining), cytotoxicity (MTT) and genotoxicity (comet assay). We also calculated the cellular Oxidative Stress Index (OSI) by measuring the Total Antioxidant Status (TAS) and Total Oxidant Status (TOS). Results: Firstly, [L(BF2)(2)] was examined in view of cytotoxic effect in seven various cell lines (HELA, DU-145, PC3, DLD-1, ECC, PNT1-A and HT-29), and then it was found that the applied complex had a mighty antiproliferative action on HT-29 cells. Thus, the most effective IC50 value turned out to be 26.49 mu M in HT-29 cell line. The present study found a tremendous efficacy of [L(BF2)(2)] on HT-29 cells, especially in terms of damage to cancer cells' DNA, and consequently caused a series of reactions leading to programmed cell death. Conclusion: The results suggest that the [L(BF2)(2)] as a novel fluorine boron hybrid complex can induce the apoptosis of HT-29 colorectal cancerous cell line and is a possible candidate for future cancer studies.
Photoprotective Effect of Flax Seed Oil (Linum Usitatissimum L.) Against Ultraviolet C-Induced Apoptosis and Oxidative Stress in Rats
(Sage Publications inc, 2012) Tuluce, Yasin; Ozkol, Halil; Koyuncu, Ismail
The aim of this study is to determine antioxidant and antiapoptotic effects of flax seed oil (FSO) on rats exposed to ultraviolet C (UVC). Malondialdehyde (MDA), protein carbonyl (PC) and reduced glutathione (GSH) levels as well as glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were measured in lens, skin and serum. In addition, beta-carotene, vitamin A, C and E contents were measured in serum, while apoptosis was determined in retina. Rats were divided into three groups as control, UVC and UVC + FSO. UVC and UVC + FSO groups were exposed to UVC light for 1 h twice a day for 4 weeks. FSO (4 ml/kg bw) was given by gavage before each irradiation period to the UV + FSO group. While MDA and PC levels of the UVC group increased compared to the control group, their levels decreased in the UVC + FSO group compared with the UVC group in skin, lens and serum. Skin GSH level decreased significantly in the UVC and UVC + FSO groups. As GPx and SOD activities of the UVC group were lower, their activities were higher in the UVC + FSO group in skin, lens and serum. There was only marked elevation of vitamin A level in the UVC group compared to the control group. Apoptosis increased in the UVC group and the UVC + FSO groups in retina. However, retinal apoptosis were lower in the UVC + FSO group compared with the UVC group. This investigation demonstrated that UVC exposure led to oxidative stress and apoptosis in rats as reflected by increased MDA, PC contents and decreased enzymatic and nonenzymatic antioxidant levels, FSO may be useful for preventing photoreactive damage.
Plasma Free Amino Acid Profile in Hiv-Positive Cases
(Bentham Science Publ Ltd, 2022) Binici, Irfan; Alp, Hamit Hakan; Karsen, Hasan; Koyuncu, Ismail; Gonel, Ataman; Celik, Hakim
Background: Increasing the sensitivity and availability of liquid chromatography tandem mass spectrometry (LC-MS/MS) devices may provide advantages in terms of revealing the changes in metabolic pathways in HIV-positive patients and elucidating the physiopathology. Introduction: The aim of this study was to determine the difference in amino acid levels between HIV-positive patients and healthy individuals by using LC-MS / MS and investigate its relationship with HIV infection. Material and Methods: Concentrations of 36 different amino acids and their derivatives were measured and compared in venous plasma samples from 24 HIV-positive patients and 24 healthy individuals by using the LC-MS/MS method (Shimadzu North America, Columbia, MD, USA). Results: HIV-positive subjects had significantly lower alanine, 1-methyl-L-histidine, valine, aspartate, cysteine, cystine, methionine, lysine, glutamine, imino acid, tyrosine, tryptophan, threonine, sarcosine, and argininosuccinic acid and significantly higher 3-methyl-L -histidine, asparagine, glutamate, and carnosine levels as compared to healthy controls. No significant differences were detected in other amino acids. Conclusion: The significant differences in amino acid profile between HIV-positive and healthy subjects may represent an auxiliary biomarker of cellular damage in asymptomatic HIV-positive patients that may be examined in more detail in further studies. It may also provide guidance for symptomatic cases in terms of the association between symptoms, clinical manifestations, and deficiency or excess of certain amino acids in the context of the complete metabolomics record of HIV-positive patients.
Ros-Mediated Genotoxicity and Apoptosis Induced by a Novel Salicylaldimine Derivatives in Human Cervical Cancer Cells
(Bentham Science Publ Ltd, 2023) Tuluce, Yasin; Mohammed, Halgurd Nadhim; Koyuncu, Ismail; Kilic, Ahmet; Durgun, Mustafa
Background Cervical cancer is one of the most common types of cancer among women. Therefore, cancer studies are underway for a new chemo-agent with more effect on cancer cells and fewer side effects on normal human healthy cells. The currently studied novel ligand L(2)b as a reduced salicylaldimine derivative was examined in seven cell lines, HeLa, DU-145, PC3, DLD-1, ECC, HT-29, and PNT1-A as a control. Aim Because of the antiproliferative ability of L(2)b, this study intends to look at the apoptotic, cytotoxic, and genotoxic activity of L(2)b on HeLa. Methods For this purpose, MTT assay is for screening cytotoxic effects, comet assay for looking for DNA damaging or genotoxicity levels, ELISA and DNA fragmentation for apoptotic measuring, AO/EB stain test for checking the rates of live, apoptotic and necrotic cells were performed. To reveal the oxidative state, OSI was assessed by total oxidant and antioxidant status ratios. FRAP assay was calculated for ferric-reducing antioxidant power, using total thiol and GSH assays to measure the antioxidant values of HeLa cells. Results Of this result, we have found a tremendous effect of L(2)b on HeLa cells, especially in raising the ROS rate, damaging their DNA, and causing a range of reactions leading to apoptosis. Conclusion In conclusion, the data predict which ligand L(2)b is capable of rising apoptosis in vitro cervical cancer cell line studied. Further cancer studies are needed to reveal the apoptosis pathways of the ligand L(2)b in the HeLa cell line and its anticancer drug potency in vivo work.
Some Medicinal Plants Counteract Alterations of Neuroendocrine Stress Response System, Oxidative and Nitrosative Stress Caused by Repeated Restraint in Rats
(Academic Journals, 2011) Ozkol, Halil; Koyuncu, Ismail; Tuluce, Yasin
Stress affects neuroendocrine stress response system, oxidative and nitrosative stress. Some reports claim that antioxidants and antistressors could attenuate these alterations. This study was realized to investigate antistress and antioxidant effects of Hypericum perforatum, Melissa officinalis, Valeriana officinalis and Passiflora incarnata extracts on repeated restraint (RS) in rats. Thirty-six rats were equally divided into six groups: A (control), B (only RS treated), C (H. perforatum + RS treated), D (M. officinalis + RS treated), E (V. officinalis + RS treated), F (P. incarnata + RS treated). Cortisol, lipid peroxidation, protein oxidation and nitric oxide (NO) levels escalated dramatically in group B compared to control, whereas almost all of them diminished remarkably again in groups C, D, E and F. Besides slight alterations of reduced glutathione content and glutathione-s-transferase, catalase activities were recorded between groups. Among vitamins only depletion of vitamin E was significant in Group B comparing to control. Interestingly administration of each plant extract led to increments of vitamins A and E even when compared to control. The results revealed that the aforementioned plant extracts has remarkable potentials to counteract repeated RS caused alterations of cortisol as well as oxidative and nitrosative stress biomarkers probably through their antistress, antioxidant and free radical defusing effects.
Subacute Effect of Cigarette Smoke Exposure in Rats: Protection by Pot Marigold (Calendula Officinalis L.) Extract
(Sage Publications inc, 2012) Ozkol, Halil; Tuluce, Yasin; Koyuncu, Ismail
This study was carried out to determine the preventive effect of Calendula officinalis L. (pot marigold) on rats exposed to cigarette smoke (CS). Rats were divided into three groups as control, CS and CS + pot marigold (PM). The rats in the CS and CS + PM groups were subjected to CS for 1 h twice a day for 23 days. PM (100 mg/kg body weight) was given to rats in the CS + PM group by gavage, 1 h before each administration period. While malondialdehyde, protein carbonyl contents and reduced glutathione level of the CS group increased, their levels diminished by PM administration. In addition, glutathione peroxidase (GPx), superoxide dismutase activities and beta-carotene, vitamins A and C levels decreased in the CS group compared to control, however activities of these enzymes and concentration of vitamins were elevated by PM supplementation. This investigation showed that administration of PM supplied relative protection against subacute CS-induced cell injury.
Therapeutic Potential of Some Plant Extracts Used in Turkish Traditional Medicine on Streptozocin-Induced Type 1 Diabetes Mellitus in Rats
(Springer, 2013) Ozkol, Halil; Tuluce, Yasin; Dilsiz, Nihat; Koyuncu, Ismail
Diabetes mellitus (DM) is known to impair many physiological functions. Some reports claim that medicinal plants can reduce these alterations caused by DM. The aim of this study was to investigate the therapeutic potential of aqueous-methanol extracts of Urtica dioica, Thymus vulgaris (TV), Myrtus communis (MC), Scolymus hispanicus (SH) and Cinnamomun zeylanicum (CZ) on streptozotocin (STZ)-induced type 1 DM in rats. Diabetes was induced via a single i.p. injection of STZ (65 mg/kg body weight). After 1 week to allow for development of diabetes, each plant extract was administered to diabetic rats separately at a dose of 100 mg/kg body weight daily for 28 days. The results showed that only SH extract significantly (P < 0.05) amended fasting blood glucose level. The lipid profile was ameliorated especially by supplementations of TV, MC and CZ extracts. Almost all plant extract treatments markedly (P < 0.05) increased reduced glutathione content and decreased lipid peroxidation levels of erythrocyte, plasma, retina and lens tissues. They also significantly (P < 0.05) amended erythrocyte catalase activity, levels of marker serum enzymes (except amylase), urea and blood urea nitrogen when compared to diabetic rats treated with nothing. Furthermore, none of the plant extracts counteracted body weight loss of diabetic rats. Our data revealed that the aforementioned plant extracts have remarkable potential to counteract DM-caused alterations, probably through their antioxidant and free radical-defusing effects.