Browsing by Author "Ozdek, Ugur"

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Ameliorative Effects of Chitosan on Fluoride-Induced Kidney Injury in Rats: a Stereological and Immunohistochemical Study
(Springer, 2025) Altindag, Fikret; Ozdek, Ugur
The present study aimed to investigate the possible protective effects of chitosan (CS) on fluoride-induced nephrotoxicity. 28 rats were divided into four groups (n = 7). The Control group received drinking water. Sodium fluoride (NaF) group received 100 mg/L NaF in drinking water. NaF + CS group received 100 mg/L NaF and 250 mg/kg/day CS by gastric gavage. CS group was given 250 mg/kg/day CS by gavage. The study period lasted 12 weeks. Total kidney volume, Bowman's capsule volume, Bowman's space volume, Tubular volume and Glomerulus volume were measured by stereological methods. Immunohistochemically, caspase-3 and TNF-alpha expressions were evaluated. Biochemically, levels of urea and creatinine were measured. In addition, a histopathological evaluation of the kidney was performed. According to the control group, an increase was observed in all stereological parameters except glomerulus volume in the NaF group. CS treatment inhibited the increase in stereological parameters. Fluoride increased expressions of caspase-3 and TNF-alpha in the kidney, and serum urea and creatine levels, but CS decreased these parameters. In addition, pathological changes in the kidney caused by fluoride such as tubular dilatation, enlargement of the Bowman's space, and deterioration in tubular epithelial cells were restored with CS treatment. The conclusions of the current study reveal that fluoride can cause nephrotoxicity and CS treatment can prevent fluoride-induced nephrotoxicity. [GRAPHICS] .
Combined Treatment of Sinapic Acid and Ellagic Acid Attenuates Hyperglycemia in Streptozotocin-Induced Diabetic Rats
(Pergamon-elsevier Science Ltd, 2021) Altindag, Fikret; Ragbetli, Murat Cetin; Ozdek, Ugur; Koyun, Necat; Alhalboosi, Jamal Khalid Ismael; Elasan, Sadi
In the present study, we aimed to investigate the effect of individual and combined treatment of sinapic acid (SA) and ellagic acid (EA) in streptozotocin (STZ)-induced diabetic rats. Rats were divided into eight groups (n = 7): Normal Control, Diabetic Control, Diabetic + Sinapic Acid, Diabetic + Ellagic Acid, Diabetic + Sinapic Acid + Ellagic Acid, Sinapic Acid, Ellagic Acid and Sinapic Acid + Ellagic Acid. Diabetic groups were injected with a single dose of 50 mg/kg STZ intraperitoneally. Rats received 20 mg/kg/day SA and 50 mg/kg/day EA intragastrically for 28 days. The numerical density of immunopositive beta-cells and volume density of pancreatic islets were calculated. Additionally, glucose and insulin levels in serum, MDA, GSH, and CAT levels of pancreatic tissue were measured. While serum glucose levels increased, serum insulin levels decreased in STZ-induced diabetic rats. But these changes in glucose and insulin were restored by individual and combined treatments of SA and EA. Also, individual and combined treatments of SA and EA increased insulin expression of beta-cells in STZ-induced diabetic rats. Moreover, these compounds improved deteriorating oxidative stress parameters in STZ-induced diabetic rats. Our study indicates that SA and EA, especially their combined treatments, can be used as an antihyperglycemic agent in diabetes.
Determination of the Levels of Serum Oxidative Indicator, Cytokine and Some Biochemical Parameters in Horses Naturally Infected With Theileria Equi
(Ankara Univ Press, 2020) Ozdek, Ugur; Oguz, Bekir; Komuroglu, Ufuk; Deger, Yeter
Equine theileriosis is considered as a serious problem because of harmful effects on the health and performance of equids. Despite the importance of this disease, there are few studies in related to clinical pathologic changes in equine theileriosis especially in horses. In this study, the oxidative stress biomarkers, cytokines, enzymes, lipid profile, electrolytes, minerals and some metabolites were evaluated in horses naturally infected with Theileria equi (n=22) and healthy (n=7). In infected horses, the serum concentrations of MDA, IFN-gamma, total protein, globulin, bilirubin (total, direct, indirect), triglyceride, glucose, iron, chloride, sodium and copper with enzyme activities of ALP, AST and GGT were found significantly higher, however concentrations of GSH, albumin, total cholesterol, HDL, TIBC, calcium, phosphorus, magnesium, potassium, zinc and manganese with enzyme activity of CAT were found lower when compared to the healthy horses (P<0.05). The changes in TNF-alpha, creatinine, urea and LDL concentrations were not statistically significant (P>0.05).The results indicate that there are significant changes in the oxidative indicator, cytokine, and biochemical parameters of horses in T. equi infection and that these changes may be useful in the evaluation of the diagnosis, prognosis and treatment of theileriosis. In addition, comprehensive studies are needed to better understand the role of cytokines in the pathogenesis of theileriosis.
The Effect of Diplotaenia Turcica Root Extract in Streptozotocin-Induced Diabetic Rats
(Walter de Gruyter Gmbh, 2020) Ozdek, Ugur; Yildirim, Serkan; Deger, Yeter
Background: Diplotaenia turcica has been used traditionally to diabetes treatment. In this study, the effects of D. turcica root extract (DT) on diabetes mellitus induced by streptozotocin (STZ) were investigated. Materials and methods: In this study, 78 male rats were used, rats were divided into 9 groups randomly. In diabetic groups, STZ was given a single dose of 45 mg/kg by intraperitoneally. DT (50,100 and 200 mg/kg) and glibenclamide (5 mg/kg) were given by orally. Blood and pancreas tissue samples were taken for biochemical and pathological tests. Results: It was found that glucose levels decreased, and insulin levels increased in the treatment groups compared with the diabetes group. In addition, only in 200 mg/kg DT dose group was found to decrease HbA1c levels. Pancreatic tissue analysis showed that MDA levels decreased and GSH levels and CAT, SOD, GSH-Px and GSH-R activities increased in diabetic rats treated with DT. Histopathological and immunohistochemical examinations of the pancreas showed significant improvements in the treatment with DT. Conclusion: These results clearly show the antioxidant property of DT. The findings of this study showed that increased doses of DT may have a therapeutic effect on STZ-induced pancreatic damage.
Effect of Meloxicam and Flunixin Meglumine on Some Kidney Parameters in Geriatric Male Rats
(Univ Zulia, Facultad Ciencias veterinarias, 2024) Komuroglu, Ahmet Ufuk; Basbugan, Yildiray; Yuksek, Nazmi; Altug, Nuri; Ozdek, Ugur; Dede, Semiha
Advanced age, called geriatrics, negatively affects the aging- related physiological changes on bodysystems. non-steroidal anti- inflammatory drugs (NSAIDs) used in geriatric pets or humans have negative effects on the kidneys. For this purpose, , the effects of Meloxicam and Flunixin Meglumine, which are NSAIDs frequently used in Veterinary Medicine, on the liver and kidney structure and function in geriatric male rats were evaluated. Twenty-four male geriatric rats (30-36 month old) and twenty-four 3-month-old young male Wistar albino rats were used in the study. Six groups were created, with eight rats in each group: young control (YC), young Meloxicam (YM), young Flunixin Meglubine (YFM), geriatric control (GC), geriatric meloxicam (GM), geriatric Flunixin Meglubine (GFM). Control groups (YC and GC) received an intraperitoneal injection of saline using the same volume as in the othergroups. Meloxicam was administered at 5.8 mgkg-1to-1 to the YM and GM groups, and Flunixin Meglumine at 2.5 mgkg-1-1 intraperitoneally to the YFM and GFM groups once a day for 5 days. Neutrophil Gelatinase-Associated Lipocalin (NGAL), Cystatin C (Cyc-c), Kidney Injury Molecule-1 (KIM-1), Interleukin-18 (IL-18), Urea, Creatinine (Crea), Albumin (Alb), and Total Protein (TP) levels, were determined in sera and urine samples. Serum NGAL, Cys-C, and KIM-1 levels in the GC group were found to be significantly higher than those in the YC group (P<0.05). P <0.05). Administration of both NSAIDs caused an increase in serum Cyc-c and NGAL levels in both young and geriatric rats (P<0.05). P <0.05). Since both Meloxicam and Flunixin Meglumine administration caused an increase in NGAL and Cys-c levels in young and geriatric rats, adjusting the drug dose and frequency of administration by evaluating the pretreatment renal function should be considered as a preventive measure.
Effects of Curcumin on Sperm Quality, Lipid Profile, Antioxidant Activity and Histopathological Changes in Streptozotocin-Induced Diabetes in Rats
(Wiley, 2020) Belhan, Saadet; Yildirim, Serkan; Huyut, Zubeyir; Ozdek, Ugur; Oto, Gokhan; Algul, Sermin
In this study, the effect of low-dose curcumin on sperm parameters, reproductive hormones, lipid profile, biochemical antioxidant parameters and the histopathological structure of the testis in diabetic male rats were evaluated. In the study, 28 male Wistar albino rats weighing 300-370 g and aged 8-10 weeks were used. Four groups of equal numbers have been created. Diabetes mellitus was induced with 45 mg/kg streptozotocin (STZ) in seven rats. Curcumin was administered to the rats in curcumin and the diabetes + curcumin group by gavage for 15 days at a dose of 10 mg/kg. Then, the rats were sacrificed. Blood samples and testis tissues were obtained, while the rats were under anaesthesia. Glucose, lipid profile, reproductive hormones, sperm parameters, biochemical antioxidant parameters and histopathological examination of the testis were performed. Abnormal sperm ratio, malondialdehyde, glucose, cholesterol, low-density lipoprotein, and triglyceride levels and caspase-3 expression were increased in diabetic rats, while the sperm motility and intensity and reduced glutathione, catalase and testosterone levels were decreased. When low-dose curcumin (10 mg/kg) was administered to diabetic rats, we found that curcumin significantly increased sperm motility and density, and decreased abnormal sperm rate according to the diabetic group. Moreover, curcumin significantly suppressed the lipid profile and increased follicle-stimulating hormone (FSH) and testosterone levels compared to the diabetic group. On testicular damage and decreased reproductive hormones caused by diabetes, curcumin may have a protective effect with indirect effect of glycaemic control by curcumin.
Effects of Silymarin on Immunohistochemical Bax and 8-Ohdg Expression, Biochemical Markers and Sperm Parameters in an Experimental Varicocele Model in Rats
(Univ Zulia, Facultad Ciencias veterinarias, 2025) Belhan, Saadet; Kayikci, Caner; Komuroglu, Ahmet Ufuk; Ozdek, Ugur; Yildirim, Serkan
In this study, the effects of silymarin on immunohistochemical Bcl-2-associatedXprotein (Bax)and8-hydroxy-2'- methoate (DMT e cide is e of t che cals used to otectdeoxyguanosinesomeagriural(8-OHdG) areas m expression, harmful o nisms. biochemical DMT markers and sperm parameters were investigated with an sidues elea ed directly or ind rectly to the envi onm nt experimentally induced varicocelemodelin rats.Thestudy use serious problems in nature DMT resid es mixed wi hthe quat c environmen adversely affect aquat c organisms a d was conducted on 36 Wistar albino rats. The distribution of rats within the group was made in an equal number. Rats in the control group were administered physiological saline daily is effect is carr ed to humans through the food chain. In thisvia oral gavage. In the sham group, an incision was made on udy, oxdative s ress respon es nd ced by DMT pe tic de inontastacusleptodacylus were nvestigated.Forthispurpose, the midline and the renal vein (located on the left) was made xidatvisible.ve A stress probe and was placed antioxidant on thisparametevein. sThe probe Thiobarbituric was coiled acid with the vein but not ligated. In the silymarin group, silymarin active s bstances (TBARS) Glutathione (GSH) Superoxidewas administered by oral gavage at adoseof 75 mg/kg 3 smu ase (SOD) c ta ase CAT) and lutathi ne pe oxidase times a week for 8 weeks. Ligation was performed on rats in PX) caused by dimethoate (DMT) pesticde in P.leptodactylus the varicocele group, unlike the sham group. Varicocele was created inthe varicocele+silymarin groups (50 mg/kg, 75 mg/ 17.5, 35, and 70 mg i-1 concentr tions at 24 and 96 h urs ere investigated Results were determined using EiISA kits. kg). Silymarin application was started 8 weeks after varicocele induction and was applied 3 days a week for 8 weeks. After the analysis, it was seen that sperm parameters were negatively ere obse ved between decreases n CAT and GPx activi ies and affected in the varicocele group. Additionally, severe caspase cr ases in TBARS leve s SPSS 24 0 packa e program one-way 3, 8-OHdG and Bax expressions were detected. Silymarin NOVA (Duncan 05) was used n the evaluation of bio hem cal administration reduced the intensity of expression and had nalyzes As a res lt,t was determined that DMT caused oxidative positive effects on spermatology. These positive effects were even more pronounced with the 75 mg dose. Based on the ress formation in P leptodac ylus and caused changes n enzyme results obtained, silymarin may have the potential to reduce both clinical and pathological symptoms in varicocele cases. tivities.
Evaluation of the Effects of Ghrelin and Metformin on Sperm Parameters, Testosterone Hormones, and Immunohistochemical and Immunofluorescent Markers in an Experimental Diabetes Model
(Tubitak Scientific & Technological Research Council Turkey, 2023) Belhan, Saadet; Huyut, Zubeyir; Yildirim, Serkan; Erkec, Ozlem Ergul; Ozdek, Ugur
Testicular dysfunction, a severe secondary complication of diabetes, negatively affects reproductive health. Ghrelin is a peptide that plays a role in many metabolic events, such as stimulating growth hormones and regulating appetite, blood pressure, and reproduction. Metformin has antiinflammatory and antioxidant properties and is widely used to treat type 2 diabetes. Therefore, in this study, to investigate the effects of ghrelin and metformin on testicular function in experimental diabetic rats given streptozotocin, placed on a high-fat diet, and that had their sperm parameters evaluated, testosterone hormone, caspase 3 and iNOS expression, and histopathological examinations were performed. In total, 38 Wistar albino male rats were randomized into 4 groups (a control group, diabetes group, diabetes + ghrelin group, and diabetes + metformin group) with different numbers. It was determined that spermatozoa concentration, motility, and testosterone levels were lower (p < 0.001), caspase 3 and iNOS expression levels (p < 0.05) were higher, and abnormal sperm rates were higher (p < 0.001) in the diabetic group compared to the other groups. Metformin and ghrelin administered to the diabetes group increased spermatozoa concentration, motility, and testosterone levels (p < 0.001) and decreased caspase 3 and iNOS expression levels, as well as abnormal sperm rates (p < 0.05). Based on these findings, we propose that ghrelin and metformin can be used to protect reproductive health in testicular dysfunction caused by diabetes.
Investigating Erythrocyte Membrane Lipid and Protein Oxidation With Na+ Activity in Caprine Anaplasmosis
(Sivar-soc Italiana veterinari Animali Reddito, 2020) Ilkaya, Salim; Deger, Yeter; Oguz, Bekir; Ozdek, Ugur
Anaplasmosis is an infectious disease that is caused by the genus Anaplasma belonging to the family Anaplasmataceae in the order Rickettsiales and is seen in the mammals inhabiting tropical and subtropical climate zones. Anaplasma ovis and A. phagocytophilum are the most remarkable species causing anaplasmosis in goats. Anaplasmosis induces both cellular and humoral immunity. As animals develop a long-term immunity against the disease, it becomes difficult to treat the disease. Immunity develops mainly depending on premunition. Increased osmotic fragility of erythrocytes in animals infected with anaplasmosis has been found, and it has been reported that this may be developed by various immune-mediated mechanisms including oxidative damage. Moreover, it may also be associated with high cell membrane ATPase activity and erythrocyte morphological changes. The aim of this study was to evaluate the effect of anaplasmosis on erythrocyte membrane malondialdehyde (MDA), advanced oxidation protein products (AOPP), Sodium-potassium adenosine 5'-triphosphatase (Na+/K(+)ATPase), and hematological and biochemical parameters in goats. For this purpose, 45 male hair goats (35 infected and 10 healthy) that were brought to the slaughterhouse of Van Metropolitan Municipality were enrolled in the study. In infected goats, the hematological and biochemical concentrations of RBC, Hb, Hct, MCV, MCH, MCHC as well as serum total protein, albumin, total cholesterol and TIBC were found significantly lower, however concentrations of of WBC, globulin, total bilirubin, direct bilirubin, triglyceride, and iron as well as enzyme activities of AST, ALT, GGT were found higher when compared to the healthy goats (p<0.05). In addition, the MDA and AOPP levels were markedly increased in erythrocyte membrane from infected animals while the Na+/K(+)ATPase enzyme activity was significantly decreased compared to the the healthy goats (p<0.05). Therefore, it can be concluded that oxidative stress in erythrocyte membrane may play an important role in the pathogenesis of anemia in caprine anaplasmosis.
Investigation of the Effect of Silymarin on Oxidative Dna Damage and Inflammatory Markers in Ischemia/Reperfusion Injury Following Experimental Testicular Torsion/Detorsion in Rats
(Tubitak Scientific & Technological Research Council Turkey, 2021) Belhan, Saadet; Yildirim, Serkan; Kayikci, Caner; Komuroglu, Ahmet Ufuk; Ozdek, Ugur; Kuscu, Yagmur
The aim of the present study was to clarify the effects of silymarin on experimental testicular ischemia / reperfusion injury. A total of 40 Wistar albino rats (10-12 weeks of age, weighing 280-300 g) were randomly divided into five groups. Control group: No surgical procedures were performed. Torsion 3 h / detorsion 3 h group; torsion 3 h / detorsion 24 h group; torsion 3 h / detorsion 3 h + silymarin (250 mg/kg) group; and torsion 3 h / detorsion 24 h + silymarin (250 mg/kg) group. In the study, 720 degrees torsion was applied to the left testicle. At the end of the study, blood was collected from the rats, and an orchiectomy was performed on the left testicles. It was found that tumor necrosis factor alpha (Tnf-alpha) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expression levels in testicular tissue increased significantly in torsion/detorsion groups, and the expression levels decreased significantly with silymarin administration. In addition, in the testicular tissue of the torsion/detorsion groups, glutathione (GSH) levels, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities decreased, while malondialdehyde (MDA) levels increased. It was found that the parameters specified were reversed with the administration of silymarin. Based on our findings, we can say that silymarin reduces testicular injury by activating antioxidant mechanisms in ischemia/reperfusion injury and minimizing the inflammatory response.
Investigation of the Effects of Calcium Fructoborate on Testicular Structure in Rats Within the Framework of Biochemical Parameters, Testosterone Hormone and Dna Damage in Cadmium Chloride Induced Toxicity
(Assoc Pharmaceutical Teachers india, 2021) Belhan, Saadet; Komuroglu, Ahmet Ufuk; Ozdek, Ugur; Mendil, Ali Sefa; Kul, Ali Riza; Dortbudak, Muhammed Bahaeddin; Gezer, Arzu
Background: The present study aims to investigate the effects of calcium fructoborate on testicular DNA damage and testicular tissue biochemical markers and serum testosterone levels after cadmium chloride administration. Materials and Methods: 28 Wistar albino rats (200-220 g) in the study were divided into 4 groups with an equal number. These groups are; Control group (No chemicals applied), calcium fructoborate (100 mg) group, Cadmium chloride (200 mg/L) + calcium fructoborate (100 mg), Cadmium chloride (200 mg/L) group. The study lasted 28 days and both chemicals were applied daily with oral gavage. Results: While 8-hydroxy-2-deoxyguanosine (8-OHdG) expression was moderate in the cadmium chloride + calcium fructoborate group, the expression in the cadmium chloride group was severe. In the cadmium chloride group, testicular tissue glutathione (GSH) and 8-OHdG levels, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and serum testosterone levels were significantly decreased compared to other groups. It is determined that the reversal of the change in the parameters listed in the calcium fructoborate group. Conclusion: The reversal of the change in the parameters listed in the calcium fructoborate group indicates the positive strength of the present chemical. It is our suggestion to transfer calcium fructoborate into life practice by conducting further clinical studies and evaluating different parameters.
Investigation of the Effects of Diplotaenia Turcica Plant Root Extract on Rat Over Tissue by Histological and Stereological Methods
(Parlar Scientific Publications (p S P), 2019) Colcimen, Nese; Ozdek, Ugur; Deger, Yeter; Altindag, Fikret; Arihan, Okan
Diplotaenia turcica is a plant species which is found in Bitlis-Van-Hakkari region and used in traditional treatments due to its medical and protective activities. Aim of this study is to investigate effects of root extract of Diplotaenia turcica plant on rat over tissue with histological and stereological methods. Four groups, with seven rats each, were used in experiments. Experimental groups were as follows; (I): control group, standard food and water was administered, Diplotaenia turcica plant root extract groups; (II): 250 mg/kg, (III): 550 mg/kg, (IV): 1000 mg/kg were administered as single dose/day with gastric gauge for 28 days. At the end of the experiments rats were decapitated and right over tissue was obtained. Histologically an increase in over medulla structure and an increase in corpus luteum and Graaf follicle number in cortex in plant administered groups were observed. Total volume comparison showed no difference between groups (p>0.05). When follicle numbers were evaluated Diplotaenia turcica administered groups showed a significant decrease in Primordial follicle number compared to control (p<0.05). No change in Primary follicle number (p>0.05) and an increase in Graaf follicle number (p<0.05) was observed. These results showed that Diplotaenia turcica increases follicular maturation by acting on this process.
Investigation of the Protective Role of Chrysin Within the Framework of Oxidative and Inflammatory Markers in Experimental Testicular Ischaemia/Reperfusion Injury in Rats
(Wiley, 2020) Belhan, Saadet; Yildirim, Serkan; Karasu, Abdullah; Komuroglu, Ahmet Ufuk; Ozdek, Ugur
This study was performed to evaluate the effect of chrysin on testicular torsion and detorsion damage in rats in terms of biochemistry, histopathology and immunohistochemistry. The study was performed on Wistar albino rats between 250 g and 300 g. A total of 40 rats were used. Five groups were created with eight rats in each group. Group 1 was the control group, and no torsion procedure was performed. In Group 2, 2 hr of torsion and 2 hr of detorsion were applied. In Group 3, 2 hr of torsion and 24 hr of detorsion were applied. In Group 4, 2 hr of torsion, 2 hr of detorsion and 50 mg/kg intraperitoneal chrysin were applied. In Group 5, 2 hr of torsion, 24 hr of detorsion and 50 mg/kg of chrysin were applied. In the torsion/detorsion groups, the study determined decreases in glutathione and testosterone levels, increases in tumour necrosis factor-alpha, interleukin-4, interleukin-6 and interleukin-10 levels, and increases in expression levels of caspase-3 and caspase-8. Chrysin application reduced malondialdehyde, tumour necrosis factor-alpha, caspase-3 and caspase-8 expression levels. We can say that chrysin can be used to reduce damage in cases of testicular ischaemia/reperfusion. For more reliable results, further clinical trials are recommended.
Protective Effects of Chitosan and Chitosan Oligosaccharide Against Oxidative Damage in Liver Tissue of Rats With Fluorine Poisoning
(Univ Karachi, 2021) Ozdek, Ugur; Komuroglu, Ahmet Ufuk; Oguz, Ahmet Regaip; Deger, Yeter
Fluorine toxicity has negative effects on soft tissue besides skeletal and dental tissues. In the present study, we have investigated the protective effect of chitosan (CS) and chitosan oligosaccharide (COS) on liver tissue of fluorine-intoxicated rats taking the antioxidant characteristics of chitosan and its derivatives into consideration. In this study, 42 male Wistar albino rats were randomly selected to determine the control and experimental fluorosis groups. Our study lasted for 12 weeks. As a consequence of the study, MDA significantly increased in the liver tissue of NaF group while some antioxidant values significantly decreased. It was detected that serum AST and LDH levels increased significantly while ALB and TP values significantly decreased in NaF group. The degenerations were identified in the liver histopathology of all fluoride- treated groups. We have concluded according to the results that chitosan oligosaccharide can be more effective compared with chitosan.
Protective Effects of Chitosan and Chitosan Oligosaccharide on Sodium Fluoride-Induced Testicular Damage in Male Rats: a Stereological and Histopathological Study
(Kafkas Univ, veteriner Fakultesi dergisi, 2021) Altindag, Fikret; Ozdek, Ugur
The aim of the present study was to investigate the potential protective effects of Chitosan (C) and Chitosan Oligosaccharide (COS) on sodium fluoride (NaF) induced testicular damage. Forty-two male Wistar rats were separated into six groups (n=7). The Control group was given drinking water without Fluoride. NaF group was given NaF (100 mg/L) in their drinking water. NaF+C group was given NaF (100 mg/L) and C (250 mg/kg/day). NaF+COS group was given NaF (100 mg/L) and COS (250 mg/kg/day). C group was given C (250 mg/kg/day). COS group was given COS (250 mg/kg/day). Rats were given C and COS by gastric gavage. As a result, the total number of spermatogonia, Leydig cells, the total volume of the testis, the total volume of germinal epithelium, the total volume of seminiferous tubules, the total length of seminiferous tubules, and the height of germinal epithelium significantly decreased in the NaF group compared to the control group (P<0.05). These parameters significantly increased in the NaF + C and NaF + COS groups compared to the NaF group (P<0.05). Also, GSH and CAT significantly decreased, while MDA significantly increased in the NaF group. C and COS alleviate these changes. These findings indicate that NaF can cause testis damage. C and COS can have cytoprotective effects against testis damage.
Protective Effects of Chrysin in Rats With Ovarian Torsion
(Kafkas Univ, veteriner Fakultesi dergisi, 2021) Dirik, Deniz; Komuroglu, Ahmet Ufuk; Kosal, Volkan; Basbugan, Yildiray; Ozdek, Ugur; Kolusari, Pinar; Keles, Omer Faruk
The objective of this study is to assess the protective effect of chrysin (CH) against ovarian torsion-detorsion injury. Thirty-two female albino rats were divided into 4 groups as Control, Torsion/Detorsion (T/D), TD-CH, and CH. Ovarian torsion was done for 3 hours on TD and TD-CH groups and then detorsion was performed. 50 mg/kg of CH was administered to the TD-CH group via oral gavage half an hour before the detorsion. Subsequently, 50 mg/kg of CH was administered via oral gavage to TD-CH and CH groups for 14 days. At the end of the experiment, blood samples were collected and ovarian tissue were taken. SOD and GPx activities and GSH and 8-OHdG levels were studied in serum and ovarian tissue. Also, IL-18, KIM-1, NGAL, Cys-C levels were studied in serum samples. GSH levels and GPx, SOD activities in both serum and ovarian tissue were significantly lower in TD group compared to the control and TD-CH groups (P<0.05), whereas the 8-OHdG level was significantly higher (P<0.05). Treatment with CH resulted in a decrease in 8-OHdG level and an increase in GSH level, GPx and SOD activities in both serum and ovarian tissue in the TD-CH group compared to TD group (P<0.05). Chrysin could ameliorate ovarian injury. Essentially, this outcome is thanks to the antioxidant, anti-inflammatory, and antiapoptotic effects of chrysin. Furthermore, it also has significant effects on DNA oxidative damage.
Protective Role of Chrysin on Doxorubicin-Induced Oxidative Stress and Dna Damage in Rat Testes
(Wiley, 2020) Belhan, Saadet; Ozkaraca, Mustafa; Ozdek, Ugur; Komuroglu, Ahmet Ufuk
This study investigated the role of chrysin (CR) in DNA damage likely to occur in the testicle and oxidative stress caused by doxorubicin (DXR). Twenty-eight rats were divided into four groups as control, DXR, DXR + CR and CR groups. Sperm parameters, oxidative status, testicular biopsy score, DNA damage and plasma testosterone levels were analysed. Noticeable reductions in sperm count, motility and testosterone were detected in the DXR group compared to controls. In addition, significant increases in malondialdehyde (MDA), catalase (CAT) and glutathione (GSH) levels, and in abnormal sperm rates were detected. Severe degenerative changes occurred in the tubules of DXR rat testes; the inter-tubular areas were oedematous. Immunofluorescence staining was conducted with 8-OhDG (8 oxo-2 '-deoxyguanosine) to evaluate DNA damage, and severe positivity was found in tubular gaps in the DXR rat testes. When the DXR + CR group was compared with the DXR group, the abnormal sperm rate was found to have decreased significantly. Positivity in the tubular space and degenerative changes in the seminiferous tubules were also diminished. We recommend the administration of CR with DXR to reduce the possible adverse effects of DXR, a medicine preferred in cancer therapy.