Protective Role of Chrysin on Doxorubicin-Induced Oxidative Stress and Dna Damage in Rat Testes

Abstract

This study investigated the role of chrysin (CR) in DNA damage likely to occur in the testicle and oxidative stress caused by doxorubicin (DXR). Twenty-eight rats were divided into four groups as control, DXR, DXR + CR and CR groups. Sperm parameters, oxidative status, testicular biopsy score, DNA damage and plasma testosterone levels were analysed. Noticeable reductions in sperm count, motility and testosterone were detected in the DXR group compared to controls. In addition, significant increases in malondialdehyde (MDA), catalase (CAT) and glutathione (GSH) levels, and in abnormal sperm rates were detected. Severe degenerative changes occurred in the tubules of DXR rat testes; the inter-tubular areas were oedematous. Immunofluorescence staining was conducted with 8-OhDG (8 oxo-2 '-deoxyguanosine) to evaluate DNA damage, and severe positivity was found in tubular gaps in the DXR rat testes. When the DXR + CR group was compared with the DXR group, the abnormal sperm rate was found to have decreased significantly. Positivity in the tubular space and degenerative changes in the seminiferous tubules were also diminished. We recommend the administration of CR with DXR to reduce the possible adverse effects of DXR, a medicine preferred in cancer therapy.