Browsing by Author "Tuluce, Yasin"

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Abcisic Acid and Gibberellic Acid Cause Increased Lipid Peroxidation and Fluctuated Antioxidant Defense Systems of Various Tissues in Rats
(Elsevier Science Bv, 2007) Celik, Ismail; Turker, Musa; Tuluce, Yasin
The study was aimed at demonstrating changes in the antioxidant defense systems [Reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT)] forming an antioxidative barrier and oxidative stress parameter (Malondialdehyde = MDA) in the various tissues of Sprague-Dawley rats which were administrated plant growth regulators (PGRs) [Abcisic acid (ABA) and Gibberellic acid (GA(3))] during 25 days. Seventy five parts per million of ABA and GA(3) as drinking water were administered orally to rats (Sprague-Dawley albino) ad libitum for 25 days continuously. The PGRs treatments caused different effects on antioxidant defense systems and MDA content of experimented rats compared to controls. The lipid peroxidation end product MDA significantly increased in the spleen and lungs of rats treated with ABA and GA(3) without significantly change in the other tissues. The GSH levels were significantly increased in the lungs and stomach of rats treated with ABA without any change in the tissues of rats treated with GA(3). Antioxidant enzyme activities such as SOD significantly increased in the spleen of rats treated with ABA and GA(3). Meanwhile, SOD significantly increased in the kidney of rats treated with GA. CAT significantly decreased in the lungs treated with ABA but did not change significantly in all the rest of rat tissues treated with both the PGRs. On the other hand, the ancillary enzyme GR activity decreased in the spleen and increased in the kidney with GA(3) treatment. The drug metabolizing enzyme GST activity significantly decreased in the heart of rats treated with GA(3) but increased in the spleen and lungs of rats treated with both PGRs. The observations presented led us to conclude that administration of subacute ABA and GA(3) promotes lipid peroxidatin content and alters in the antioxidative systems in the rat's various tissues. These data, along with changes, suggest that the PGRs produced oxidative stress in rats during the period of a 25-day subchronic exposure. (C) 2007 Elsevier B.V. All rights reserved.
Ameliorative Influence of Urtica Dioica L Against Cisplatin-Induced Toxicity in Mice Bearing Ehrlich Ascites Carcinoma
(Taylor & Francis Ltd, 2012) Ozkol, Halil; Musa, Davut; Tuluce, Yasin; Koyuncu, Ismail
Cisplatin (CP) is a widely used cytotoxic agent against cancer, and high doses of CP have been known to cause nephrotoxicity and hepatotoxicity. Some reports claim that antioxidants can reduce CP-induced toxicity. This study investigated the hepatoprotective, nephroprotective, and antioxidant activity of Urtica dioica L methanolic extract (UDME) against CP toxicity in Erhlich ascites tumor (EAT)-bearing mice. Levels of serum hepatic enzymes, renal function markers, and oxidant/antioxidant parameters of liver tissue were measured. Mice were inoculated with EAT on day 0 and treated with nothing else for 24 hours. After a single dose of CP administration on day 1, the extract was given at the doses of 50, 100, 200, and 400 mg/kg body weight daily during 6 days. Almost all doses of UDME performed a significant (P<0.05) preventive role against CP toxicity by decreasing aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, blood urea nitrogen, creatinine, lipid peroxidation, protein oxidation levels, and myeloperoxidase activity, as well as increasing reduced glutathione content, superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase activities. This suggests that UDME has a protective capacity and antioxidant activity against CP toxicity in EAT-bearing mice, probably by promoting antioxidative defense systems.
Anthocyanin-Rich Extract From Hibiscus Sabdariffa Calyx Counteracts Uvc-Caused Impairments in Rats
(Taylor & Francis Ltd, 2015) Ozkol, Hatice Uce; Koyuncu, Ismail; Tuluce, Yasin; Dilsiz, Nihat; Soral, Sinan; Ozkol, Halil
Context: Ultraviolet radiation (UV) was reported to cause oxidative stress. Hibiscus sabdariffa L. (Malvaceae) calyx is commonly used in traditional Asian and African medicines and possesses strong antioxidant capacity due to its anthocyanin (ANTH) content. Objective: This study researched the possible protective role of Hibiscus sabdariffa calyx extract (HSCE) in UVC exposure of rats. Material and methods: Levels of serum enzymes, renal function tests, and some oxidant/antioxidant biomarkers of skin, lens, and retina tissues were monitored. Rats were exposed to UVC 4 h daily for 40 d and simultaneously received HSCE containing 2.5, 5, and 10 mg doses of ANTH in drinking water. Results: Significant (p < 0.05) increases in the levels of serum aminotransferases, lactate dehydrogenase, urea, creatinine, and uric acid were noted after UVC exposure. In skin, lens, and retina tissues, total oxidant status, oxidative stress index, lipid peroxidation, and protein oxidation escalated markedly (p < 0.05) whereas total antioxidant status, reduced glutathione, and superoxide dismutase decreased dramatically (p < 0.05) related to UVC. Co-administration of HSCE with each ANTH dose significantly (p < 0.05) reversed aforementioned parameters (except total oxidant status) almost in all tissues. The LD50 of HSCE in rats was determined to be above 5000 mg/kg. Discussion and conclusion: Our data revealed that HSCE has a remarkable potential to counteract UVC-caused impairments, probably through its antioxidant and free radical-defusing effects. Therefore, HSCE could be useful against some cutaneous and ocular diseases in which UV and oxidative stress have a role in the etiopathogenesis.
Antioxidant and Immune Potential Marker Enzymes Assessment in the Various Tissues of Rats Exposed To Indoleacetic Acid and Kinetin: a Drinking Water Study
(Academic Press inc Elsevier Science, 2006) Celik, Ismail; Tuluce, Yasin; Turker, Musa
In the present study, the influence of two different PGRs, indoleacetic acid (IAA) and kinetin (Kn) on immune potential enzymes, adenosine deaminase (ADA) and myeloperoxidase (MPO), and antioxidant defense enzymes such as glutathione peroxidase (GPx), glutathione reductase (GR), and superoxide dismutase (SOD) in various tissues of rats were investigated during the treatment as a drinking water model. 100 ppm of IAA and Kn as drinking water were administered orally to rats (Sprague-Dawley albino) ad libitum for 21 days continuously. The PGRs treatments caused different effects on the immune potential and antioxidant defense enzymes of experimented rats compared to controls. Results show that IAA caused a significant decrease in GR activity in the lungs and liver and an increase in the spleen. Also, IAA caused a significant decline in GPx activity in the lungs and an increase in the heart. SOD was significantly reduced in the heart, while increased in the lungs. Furthermore, IAA caused a significant decrease in ADA activity in the heart and blood whereas an increase in the kidney and spleen. MPO activity was also significantly increased in the heart by IAA treatment. The activity of enzymes were also seriously affected by Kn; GR activity decreased in the lungs, brain, and blood while GPx activity decreased in the spleen, brain, and heart. ADA activity was also significantly reduced in the blood whereas MPO activity rose in the spleen. In addition, SOD activity lowered in all tissues except for lungs where a significant increment was determined. As a conclusion, the results indicate that PGRs might affect on antioxidant and immune potential enzymes. These data, along with the determined changes suggest that PGRs produced substantial systemic organ toxicity in the erythrocyte, liver, brain, heart, lungs, spleen, and kidney during the period of a 21-day subacute exposure. (c) 2006 Elsevier Inc. All rights reserved.
Antiproliferative and Apoptotic Role of Novel Synthesized Cu(Ii) Complex With 3-(3 Benzenesulfonamide in Common Cancer Models
(int inst Anticancer Research, 2018) Tuluce, Yasin; Gorgisen, Gokhan; Gulacar, Ismail Musab; Koyuncu, Ismail; Durgun, Mustafa; Akocak, Suleyman; Kaya, Zehra
Background/Aim: Chemotherapeutic treatment options are often ineffective due to the development of resistance in cancer cells. Therefore, developing new anti-cancer agents is crucial for cancer treatment. Some triazine derivatives, their complexes and Copper(II) have anti-cancer effects on cancer cells. In this study, we aimed to determine the anti-proliferative effect of the novel synthesized Cu(II) complex with 3-(3-(4-fluorophenyl)triaz-1 -en- 1-yl) benzene-sulfonamide compound on the common cancer cell lines HeLa, MDA-MB-231, A2780 and MCF7. Materials and Methods: Common cancers cell lines were treated with copper complexes. Cell viability and apoptotic gene expression were examined. Results: Novel synthesized copper complex led to decreased viability of all cell lines. It also induced apoptosis via increasing the expression of caspase-3, caspase-9, Bax and p53 proteins and decreasing ERK expression. Conclusion: The novel synthesized copper complex has a significant inhibitory effect on the viability of cancer cell lines and can be considered as an antitumor agent for further studies.
The Apoptotic, Cytotoxic and Genotoxic Effect of Novel Binuclear Boron-Fluoride Complex on Endometrial Cancer
(Springer, 2017) Tuluce, Yasin; Lak, Pawan Tareq Ahmed; Koyuncu, Ismail; Kilic, Ahmet; Durgun, Mustafa; Ozkol, Halil
Endometrial cancer (EC) is one of the most common types of gynecologic cancer of the female genital tract; it considered being the fourth leading death factor among other types of cancer. Therefore, developing new anti-cancer agents are crucial for cancer treatment. Based on the potential of Schiff based complexes for the induction of apoptosis, Schiff base compounds, and their metal complexes displayed excellent anticancer properties. In this current study, antiproliferative activity of [L(BF2)(2)] as a novel binuclear boron-fluoride complex was examined to preliminary research in eight different cell lines, HELA, DU-145, PC3, DLD-1, ECC-1, PNT1-A, HT-29, and MCF-7, it was found to have a potent, suppressive effect on human endometrial adenocarcinoma cell line ECC-1. Based on this data, later investigated its apoptotic, cytotoxic, and genotoxic properties on human endometrial adenocarcinoma cell line ECC-1 in different concentrations. Apoptotic and cytotoxic tests such as single cell gel electrophoresis assay (comet assay), DNA fragmentation laddering, acridine orange test for DNA damage, and ELISA for apoptotic measurement was performed. We also gauged the oxidative status by evaluating total antioxidant status (TAS) and total oxidant status (TOS). Oxidative stress index (OSI) was calculated too. As a result [L(BF2)(2)] has been found to have a marvelous effect on ECC-1 cells, especially in damaging their DNA and cause a series of reactions lead to apoptosis. Taken together, it suggests that the [L(BF2)(2)] complex can induce the apoptotic pathway of endometrial cancer cells and is a possible candidate for future cancer treatment studies.
Assessment of Redox Homeostasis Via Genotoxicity, Cytotoxicity, Apoptosis and Nrf-2 in Colorectal Cancer Cell Lines After Treatment With Ganoderma Lucidum Extract
(Taylor & Francis Ltd, 2024) Tuluce, Yasin; Keles, Ahmet Yasin; Kostekci, Sedat
This study aimed to investigate the cytotoxic and apoptotic effects of Ganoderma lucidum, Pleurotus ostreatus, Pleurotus eryngii, and Inonotus hispidus fungal extracts on HT-29 and HCT-116 colorectal cancer cell lines and to search the DNA damage and oxidative stress caused by these extracts. Accordingly, mushroom extracts were applied to colorectal cancer cell lines in vitro, and the IC50 result was obtained with the MTT test. According to the IC50 result, Ganoderma lucidum extract had the most effective cytotoxicity value among all used mushroom extracts. TAS, TOS, and NRF-2 tests were used to investigate the molecular effect of Ganoderma lucidum extract on oxidative stress; the DNA ladder test was performed to assess DNA damage, the Scratch assay method was applied for cell migration analysis, and the colony assay was used to determine the colony formation potential of the cells. The results showed that Ganoderma lucidum mushroom extract reduces cell proliferation, colony formation, and NRF-2, induces DNA damage, slows cell migration, and increases oxidative stress. This study shows that Ganoderma lucidum mushroom extract reduces cell proliferation through damaging cellular DNA and has a cytotoxic effect in colorectal cancer cell lines.
Attenuation of Uvc-Induced Toxicity by Linum Usitatissimum L Oil in Rats: Monitoring of Some Hematological and Biochemical Constituents
(Parlar Scientific Publications (p S P), 2011) Ozkol, Halil; Tuluce, Yasin; Koyuncu, Ismail
The aim of this study was to investigate the protective effect of Linum usitatissimum L. seed oil (LUSO) against ultraviolet C (UVC) exposure through monitoring some hematological and biochemical constituents of rats. For this purpose, rats were divided into three groups: control, UVC and UVC+LUSO. The rats of UVC and UVC+LUSO groups were subjected to UVC during 1 h twice a day for 4 weeks. LUSO (4 ml/kg b.w.) was given to rats of UVC+LUSO group by gavage before each irradiation period. Results showed that lipase and cholesterol levels were markedly higher in UVC group than control. Lipase decreased significantly, and cholesterol diminished slightly with combined treatment of UVC and LUSO. While the level of red cell distribution width (RDWc) decreased in UVC group, it increased significantly after LUSO supplementation. Apart from that, calcium and monocyte levels increased markedly in UVC+LUSO group compared with control and UVC groups. Significant decreases were determined in amounts of white blood corpuscles and lymphocytes as well as an increase was observed in monocyte percentage of UVC+LUSO group compared to control. This investigation showed that treatment of LUSO with UVC supplied relative protection against UVC injury and perfected some blood parameters significantly, even when compared with control group.
A Biochemical Study on the Protective Potential of Centaurium Erythraea L. in Rats at Acute Aspirin Exposure
(Parlar Scientific Publications (p S P), 2011) Ozkol, Halil; Tuluce, Yasin; Koyuncu, Ismail; Balahoroglu, Ragip
The aim of this study was to determine protective effect of Centaurium erythraea L. on rats exposed to aspirin (ASA). The protective influence of the 50% aqueous-ethanolic Centaurium erythraea L. extract (AECE) (100 mg/kg body weight) was investigated in rats exposed to a single dose of 200 mg/kg body weight ASA administration. Twenty-one Sprague-Dawley albino rats were distributed into three groups equally (n=7) as follows: A (control), B (only ASA-treated) and C (ASA+AECE-treated) group. At the end of 4 h ASA administration to groups B and C, some serum biochemical constituents were assigned and compared between the groups. Results showed that levels of aspartate aminotransferase, alanine aminotransferase, creatinine and potassium increased in group B compared with the control group, but they were decreased significantly with AECE supplementation. While lipase activity decreased only in group B, level of uric acid decreased both in groups B and C compared with control group. In conclusion, the current trial has shown that treatment of AECE with ASA supplied relative protection against acute ASA toxicity through ameliorating some blood parameters.
Calendula Officinalis L. Ameliorates Levels of Some Blood Constituents and Body Weight Loss in Passive Cigarette Smoke Exposure of Rats
(Parlar Scientific Publications (p S P), 2011) Ozkol, Halil; Tuluce, Yasin; Koyuncu, Ismail
The aim of this study was to investigate the protective effect of Calendula officinalis L. (CO) on rats exposed to cigarette smoke (CS). For this purpose twenty one albino male Sprague Dawley rats were randomly divided into three groups as control, CS and CS+CO. The rats of the CS and CS+CO groups were subjected to CS during 1 h twice a day for 23 days. CO (100 mg/kg body weight) was given to the rats of the CS+CO group by oral gavage one hour before each administration period. Protective effect of CO was detected by comparing some serum biochemical parameters and mean body weight changes of groups. While aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CK) levels of CS group increased, amylase and lipase decreased significantly compared to control. AST, LDH and CK levels reversed to nearly their control group values by CO supplementation. There was a significant reduction in the HDL level of the CS group compared to control. But its level increased markedly in the CS+CO group. With regard to calcium, its amount escalated significantly in the CS and CS+CO groups compared with control. Administration of CO caused an amendment in the body weight loss. In conclusion, this study revealed that CO could relatively combat the hazardous effects of CS and thus may help to reduce development risk of some disorders related to CS.
Chronotypes and Oxidative Stress: Is There an Association
(Taylor & Francis Ltd, 2012) Selvi, Yavuz; Ozkol, Halil; Tuluce, Yasin; Besiroglu, Lutfullah; Ozdemir, Pinar Guzel
Human studies suggest that free radicals and antioxidant enzymes can alter according to age, lifestyle, environment, and habits. Individuals having a marked circadian preference, that is, morning type or evening type, differ on a number of psychological, behavioral, and biological variables. The aim of this study was to determine whether chronotype impacts some parameters of oxidant and antioxidant status. For this purpose, glutathione peroxidase (GSH-P-x), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities and the levels of reduced glutathione (GSH) and malondialdehyde (MDA) were measured in 96 healthy volunteers (including 32 morning-type, 32 intermediate-type, and 32 evening-type individuals), aged between 21 and 26 years. There were no significant relationships between the chronotypes with regard to oxidant and antioxidant parameters. Our results indicated that the levels of GSH and MDA as well as the activities of GSH-P-x, MPO, and SOD were not influenced by the individual circadian differences in our sample. These results were discussed using the theoretical concepts of age-related factors, and suggestions for further research presented.
Comparison of Superoxide Dismutase, Glutathione Peroxidase and Adenosine Deaminase Activities Between Respiratory and Nocturnal Subtypes of Patients With Panic Disorder
(Karger, 2012) Ozdemir, Osman; Selvi, Yavuz; Ozkol, Halil; Tuluce, Yasin; Besiroglu, Lutfullah; Aydin, Adem
Objective: There is mounting evidence indicating that oxidative and inflammatory processes may have an important role in the pathogenesis of panic disorder (PD). PD is a heterogeneous disease, and panic attacks are divided according to the different symptom clusters as respiratory, nocturnal, non-fearful, cognitive, or vestibular subtypes. The aim of this study was to compare whole-blood and serum superoxide dismutase (SOD), glutathione peroxidase and adenosine deaminase activities in PD patients with/without nocturnal, respiratory subtypes and healthy subjects. Methods: The study was conducted including 60 patients with PD and 30 healthy control subjects. The Panic Attack Symptom Checklist, Panic and Agoraphobia Scale, Hamilton Depression Rating Scale and Hamilton Anxiety Rating Scale were administered to the patients. Biochemical analyses were performed after all the blood samples were collected. Results: We found that whole-blood SOD and glutathione peroxidase activities of patients were significantly lower and adenosine deaminase activities of patients were higher than those of healthy controls. There were no statistically significant differences between respiratory and nocturnal subtypes. In addition, there were no marked relationships between the duration of illness and panic-agoraphobia scores of patients with nocturnal subtypes. Hamilton Depression Rating Scale and Hamilton Anxiety Rating Scale scores of patients with the nocturnal subtype were markedly higher than those of patients without the nocturnal subtype. Conclusion: The results suggest that oxidative and inflammatory processes may play a role in the pathophysiology of PD. These findings may support the idea that both nocturnal and respiratory subtypes of PD have different symptom clusters of the same disease. Copyright (C) 2012 S. Karger AG, Basel
The Cytotoxic, Apoptotic and Oxidative Effects of Carbonic Anhydrase Ix Inhibitor on Colorectal Cancer Cells
(Springer/plenum Publishers, 2018) Tuluce, Yasin; Ahmed, Bewar Ali; Koyuncu, Ismail; Durgun, Mustafa
Colorectal cancer (CRC) is the third most common tumor, malignant and has developed one of the main reasons of cancer mortality. According to studies conducted recently; carbonic anhydrase 9 (CAIX) is an especially attractive target for cancer therapy, in part since it is limited way expressed in normal tissues on the other hand in a wide variety of solid neoplasia are overexpressed. The aim of this study was to appreciate the effects of CAIX inhibitor, namely novel synthesized sulfonamide derivative (H-4i) with high affinity for CAIX, in CAIX-positive human colorectal cancer cell (HT-29) and CAIX-negative human normal embryonic kidney cell line (HEK-293). For this reason, we planned to investigate apoptotic, cytotoxic and oxidative stress activity of H-4i on HT-29 and HEK-293 cell lines. Cell viability determined by WST-1 assay afterwards IC50 values, apoptosis and cell cycle induction measured by flow cytometric analysis, intracellular free radical induction performed by reactive oxygen species (ROS) analyses. The IC50 value of the sulfonamide derivative compound was found to be very low, especially in HT-29 cells, when compared to human normal cells. This research found that H-4i significantly increased cytotoxicity and ROS production, caused significant signs of apoptosis level. High level of ROS and apoptosis lead to arrest the cell cycle and reduce cell survival. The most obvious finding to emerge from the analysis that novel synthesized sulfonamide derivative H-4i is effective on HT-29 more than HEK-293. Therefore, novel derivative H-4i might be used as an anti-cancer potential compound on CRC.
Design, Synthesis, and Evaluation of Pyrrolopyrazine-Substituted Benzoxazole/Benzothiazole Derivatives Targeting Aurora Kinase a in Mcf-7 Cells
(Wiley-V C H Verlag Gmbh, 2025) Kuzu, Burak; Kostekci, Sedat; Karakus, Fuat; Tuluce, Yasin
This study reports the synthesis, characterization, and biological evaluation of 19 benzoxazole/benzothiazole hybrids (19a-s). The compounds were synthesized through a multi-step process and structurally confirmed via NMR, elemental, and MS analyzes. Their antiproliferative effects were assessed on MCF-7 breast cancer and HME1 healthy epithelial cells. MTT assays identified 15 compounds with significant cytotoxic activity, among which 19e, 19g, 19i, 19j, and 19k exhibited high selectivity for MCF-7 cells. ELISA results demonstrated that 19g, 19i, 19j, and 19k significantly reduced AURKA protein levels in MCF-7 cells, while sparing healthy cells, suggesting their role in inhibiting cancer cell proliferation. These findings highlight 19g, 19i, 19j, and 19k as promising selective AURKA-targeted agents for breast cancer therapy.
Determination of Toxicity of Subacute Treatment of Some Plant Growth Regulators on Rats
(Wiley, 2007) Celik, Ismail; Tuluce, Yasin
The effects of some plant growth regulators (PGRs), 2,3,5-triiodobenzoic acid (TIBA), Naphthaleneacetic acid (NAA) and 2,4-Dichlorofenoxyacetic acid (2,4-D), at sublethal concentrations on antioxidant defense system [glutathione peroxiclases (GPx), reduced glutathione (GSH), glutathione reductase (GR), glutathione-S-transferase (GST) and catalase (CAT], immune potential enzymes [adenosine deaminase (ADA) and myeloperoxidase (MPO)], and lipid peroxidation content [Malondialdehyde, (MDA)] were investigated in lung and speen tissues of rats. Sprague-Dawley albino rats were exposed to 0, 50, or 100 ppm (parts per million) TIBA, NAA, or 2,4-D in drinking water ad libitum for 25 days continuously. According to the results, MDA concentration significantly increased in the tissues treated with 100 pprn dosage of NAA or 2,4-D without any change in the tissues of rats treated with both dosage of TIBA. The GSH depletion in the spleen tissue of rats treated with both the dosage of NAA and 2,4-D were found to be significant. Also, GSH level in the spleen was significantly reduced with 100 ppm of 2,4-D and NAA. The activity of antioxidant enzymes were also seriously affected by PGRs; GPx significantly decreased in the lung of rats treated with both dosages of the PGRs, whereas GPx activity in the spleen were significantly increased with 100 ppm dosage of 2,4-D and NAA. On the other hand, CAT activity significantly decreased in the lung of rats treated with both dosages of NAA, 100 ppm of 2,4-D and 50 ppm of TIBA, and also in the spleen treated with 50 ppm NAA and 2,4-D. The ancillary enzyme GR activity significantly decreased in the spleen with both doses of the PGRs, also in the lung treated with both dosages of 2,4-D, 50 ppm of NAA and 100 ppm of TIBA. The drug metabolizing enzyme GST activity significantly reduced in the lung of rats treated with both dosages of the PGRs and also in the spleen treated with 100 ppm dosage of 2,4-D and TIBA and 50 pprn of NAA. Meanwhile, immune potential enzyme MPO activity significantly increased in the spleen of rats treated with both doses of NAA and TIBA whereas ADA activity significantly decreased in the spleen of rats treated with 100 ppm dose of NAA and TIBA. The observations presented led us to conclude that the administrations of subacute NAA, 2,4-D, and TIBA promote MDA content, inhibit the antioxidative defense system and activate or inhibit immune potential enzymes in the rat's spleen and lung tissues. These data suggest that PGRs produced substantial organ toxicity in the lung and spleen during the period of a 25-day subacute exposure. (C) 2007 Wiley Periodicals, Inc.
The Effect of a Bis-Structured Schiff Base on Apoptosis, Cytotoxicity, and Dna Damage of Breast Cancer Cells
(Wiley, 2022) Tuluce, Yasin; Hussein, Azhee Ibrahim; Koyuncu, Ismail; Kilic, Ahmet; Durgun, Mustafa
Developing new anticancer agents are crucial for cancer treatment. Antiproliferative activity of L1H as a bis-structured Schiff base was subjected to preliminary research in eight different kinds of cell lines by the cell viability method using different concentrations to determine their inhibitory concentration. L1H demonstrated the highest cytotoxicity in human breast cancer cell line MCF-7. In this perspective, the MCF-7 cell line was cultured for the examination of different molecular techniques, including MTT, apoptosis analysis by enzyme-linked immunosorbent assay (ELISA), and comet assay. Moreover, the DNA ladder, acridine orange/ethidium bromide as another apoptotic cell analysis, markers of oxidative stress, and total antioxidant status, total thiol, and GSH as nonenzymatic antioxidants assay were conducted. The above techniques have proven that L1H is a growth inhibitor effect when compared to cisplatin as a positive control in human breast cancer cells, especially those affected by L1H. The findings clearly show that L1H evaluated in MCF-7 cell lines causes rising or induced apoptosis, DNA damage, diminished antioxidant status against the increase of oxidized protein, and prevents cell proliferation. Manifold evidence supported our hypothesis that L1H has a potential therapeutically improved effect against the MCF-7 cell line, and then without a doubt is a suitable candidate drug for investigating cancers next.
The Effect of Anti-Ccp Antibodies on Synovial Fluid Oxidant and Anti-Oxidant Activities in Patients With Rheumatoid Arthritis
(Oxford Univ Press, 2011) Ediz, Levent; Tuluce, Yasin; Ozkol, Halil; Hiz, Ozcan; Gulcu, Elif; Toprak, Murat
The Effects of Diet Supplemented With the Black Cumin (Nigella Sativa L.) Upon Immune Potential and Antioxidant Marker Enzymes and Lipid Peroxidation in Broiler Chicks
(Medwell online, 2008) Sogut, Bunyamin; Celik, Ismail; Tuluce, Yasin
This study was carried out to investigate whether Nigella saliva (NS) could decrease the lipid peroxidation [Malondialdehyde = MDA], increase the anti-oxidant defence system [Reduced Glutathione (GSH), Catalase (CAT) and Glutathione-S-Tranferase (GST)] and also posses the immunopttential [Adenosine Deaminase (ADA) and Myeloperoxidase (MPO)]. Totally, 100 Ross 308 birds, I day old, were used. Chicks were divided into 4 experimental groups, (control, 3, 5 and 7% containing Nigella saliva) each containing 25 broiler chicks. While, the control group received normal broiler fattening feed, the treatments groups were fed with the feed containing 3, 5 and 7% grinded Nigella saliva seed and all groups fed and watered ad libitum for 6 weeks during experiment. At the end of the 6 weeks experiment, liver samples were taken to measure MDA, GSH, CAT, GST, ADA and MPO. While, treatment with N. saliva decreased the liver MDA content, increased the activities of CAT, GST, ADA and MPO significantly, but increased the level of GSH lightly in comparison to control. It was concluded that N. saliva might be used to potent of antioxidant and immune system and also prevent liver from oxidative stress resulting lipidperoxidation.
Effects of Subacute Treatment of Thiourea on Antioxidant and Immunopotential Marker Enzymes and Lipid Peroxidation of Rats: a Drinking Water Study
(Asian Journal of Chemistry, 2006) Celik, Ismail; Suzek, Huseyin; Tuluce, Yasin
The effect of subacute 25 days treatment of thiourea, an inhibitor of diene conjugate formation as hydroxyl radical scavengers were tested as terminators of lipid peroxidation (malondialdehyde = MDA), antioxidant [reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT] and immunopotential [adenosine deaminase (ADA) and myeloperoxidase (MPO) defense systems marker enzymes in the liver tissue of rats under laboratory conditions. 250 ppm of thiourea was administered orally to 8 rats ad libitum during the tests for 25 days consecutively. Malondialdehyde content, antioxidant and immunopotential marker enzyme of liver were determined after treatment. According to results, while the treatment of thiourea caused significant decrease in MDA content, GSH level, GR activity and immunopotentical enzyme activities decreased in comparison to control rats. CAT, GPx, SOD and GST activities did not change significantly, nese results suggest that thiourea may not only reduce oxidative damage by induced oxygen reactive radicals in animals, but affect immunopotential enzymes too.
Elevation Protective Role of Camellia Sinensis and Urtica Dioica Infusion Against Trichloroacetic Acid-Exposed in Rats
(Wiley, 2007) Celik, Ismail; Tuluce, Yasin
This study was designed to investigate the protective effects of two traditionally used Turkish medicinal plants, Camellia sinensis (CS) and Urtica dioica L. (UD), beverages used against chemical carcinogen trichloroacetic acid (TCA)-exposure in rats. The preventive potential of the plant infusions was evaluated by measuring the level of serum marker enzymes, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), acid phosphatase (ACP), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH); antioxidant defense systems, reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT); and lipid peroxidation (malondialdehyde, MDA) content in various organs of rats. Twenty four healthy rats were randomly allotted into four experimental groups: A (untreated control), B (only TCA-treated), C (TCA + CS treated) and D (TCA + UD treated). At the end of the 50 days, the plant infusions possessed chemoprotective effects, deduced by the remaining TCA-induced increased serum damage marker enzyme, lipid peroxidation and antioxidative system in rats compared with those of the control and TCA-exposed rats. According to the results, while the levels of AST, ALT and CPK increased in group B, no significant changes were observed in groups C and D. The MDA content slightly increased in tissues of all groups, being higher in group B. Antioxidant enzyme activities such as SOD and CAT increased significantly in the brain, liver and kidney of group B while they did not change significantly except for in the kidney in groups C and D. The GSH level and the ancillary enzyme GR activity did not change significantly in organs of all groups. On the other hand, the drug metabolizing enzyme, GST, activity decreased significantly in the brain, liver and kidney of group D while slight changes were observed for the other groups. The results revealed that TCA exposure induced oxidative stress in rat tissues, however, in plant beverage supplemented groups, a significant protective effect of CS and UD against TCA-induced oxidative injury was recorded. Hence, the study revealed that the constituents present in CS and UD impart protection against carcinogenic chemical induced oxidative injury that may result in the development of cancer. Also the observations, along with changes, suggest that both CS and UD may possess preventive potential during a 50-day protective exposure. Copyright (c) 2007 John Wiley & Sons, Ltd.