Van Yüzüncü Yıl Üniversitesi

Permanent URI for this communityhttps://hdl.handle.net/20.500.14720/1

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Selective Solid-Phase Extraction and Determination of Profenofos from Food and Water Samples Using Surface Molecularly-Imprinted Bentonite
(Academic Press Inc Elsevier Science, 2026) Hamosi, Bafreen Jaafar Mohammed Saleem; Gursoy, Tulay; Karakoyun, Necdet; Zengin, Adem; Bilici, Mustafa
In this study, a surface-imprinted molecularly imprinted polymer (MIP) was prepared on bentonite (BNT) to create a selective solid-phase extraction (SPE) sorbent for detecting profenofos (PFF), a commonly used organophosphorus pesticide. The MIP-BNT material was produced through surface-initiated free-radical polymerization, and structural analyses confirmed the presence of specific binding sites, uniform morphology, and an enhanced surface area. Adsorption behavior followed a pseudo-second-order kinetic model (R2= 0.9965), while equilibrium data showed strong agreement with the Langmuir isotherm (R2= 0.9979), indicating monolayer adsorption. Key SPE parameters-including pH, sorbent amount, desorption time, and eluent volume-were systematically optimized. The final conditions (pH 6.0, 10 mg sorbent, 25 min desorption, 150 mu L eluent) ensured high recovery and repeatability. When coupled with UV-vis spectrophotometry, the optimized MIP-SPE method provided a wide linear range (0.1-270 mu M), a low detection limit (0.03 mu M), and stable performance over at least eight reuse cycles. Recovery experiments conducted in food and water matrices yielded values between 94.01 % and 100.42 %, with RSD values below 5 %, and results were consistent with HPLC measurements. Overall, the developed approach presents a selective, cost-effective, and environmentally friendly alternative for routine PFF analysis.
Strategic Engineering of Imidazopyridine-Benzoxazole Hybrids Targeting Microtubule Dynamics: Comprehensive Inhibition of the Metastatic Cascade
(Academic Press Inc Elsevier Science, 2026) Kuzu, Burak; Cakir, Mustafa; Acikgoz, Eda
A series of twenty novel imidazopyridine-benzoxazole hybrid (Imp1-20) derivatives was designed and synthesized, and their antiproliferative activities were evaluated against MDA-MB-231 breast and DLD-1 colorectal cancer cell lines. Among them, Imp-18 and Imp-20 emerged as the most potent candidates, with low micromolar to nanomolar IC50 values and significant reductions in cancer cell adhesion and colony formation. Flow cytometry analyses demonstrated that both compounds induced apoptosis and promoted cell-cycle arrest, reflected by Sub-G1 accumulation and perturbations in G1/G0 and G2/M phases. Immunofluorescence imaging of beta-tubulin confirmed that Imp-18 and Imp-20 compromise microtubule integrity, with Imp-18 displaying stronger tubulin-disrupting activity than nocodazole. The resulting microtubule destabilization was consistent with mitotic arrest and activation of apoptotic signaling pathways. Additionally, both compounds markedly inhibited cancer cell migration, indicating an ability to impair metastatic behavior. Overall, these findings identify Imp-18 and Imp-20 as promising microtubule-targeting agents with robust anticancer potential, providing a strong basis for further mechanistic studies and structural optimization within the framework of medicinal and bioorganic chemistry.
Syringic Acid Mitigates Scopolamine-Induced Cognitive Impairment by Regulating PSD-95 and GSK-3β and by Preventing Neurodegeneration in an Alzheimer-Like Rat Model
(Academic Press Inc Elsevier Science, 2026) Altindag, Fikret; Bayir, Mehmet Hafit; Alhalboosi, Jamal Khalid Ismael; Yildizhan, Kenan
Alzheimer's disease (AD) is a disorder characterized by progressive cognitive impairment. Syringic acid (SA) is a phenolic compound with many beneficial effects, such as antioxidant, anti-inflammatory, anti-diabetic, anticarcinogenic, and neuroprotective. Our study aimed to investigate the effects of SA (50 mg/kg/day) on scopolamine (SCO)-induced AD-like condition in rats. Immunohistochemical evaluation was performed using antibodies to postsynaptic density protein 95 (PSD-95), Glycogen synthase kinase-3 beta (GSK-3 beta), TNF-alpha, and caspase-3. The hippocampus was stained with Hematoxylin-Eosin, and the total number of hippocampal neurons and hippocampal volume were calculated using the stereological method. The Y-maze task behavioral test was performed. SCO decreased PSD-95 expression while increasing GSK-3 beta, TNF-alpha, and caspase-3 expression. SA treatment increased PSD-95 expression while decreasing GSK-3 beta, TNF-alpha, and caspase-3 expression. Compared to the control group, the number of hippocampal neurons was significantly decreased in the Alzheimer's group, but the number of neurons in the SA group was significantly higher than in the Alzheimer's group. Hippocampal volume was lower in the Alzheimer's group, although there was no statistical difference between the groups. SA also improved SCO-induced cognitive impairment. Our study findings suggest that SA may mitigate SCO-induced cognitive impairment in the AD rat model, modulating PSD-95 and GSK-3 beta and decreasing neuroinflammation and apoptosis.

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