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Young Plasma Transfer Enhances Antioxidant Defense and Preserves Structural Integrity in Aged Lung Tissue
(2026) Düvenci Birben, Özlem; Teker, Hikmet Taner; Keskin, Seda; Baba, Burcu; Koçpınar, Enver Fehim; Yıldırım, Vesila; Kabayer, Sevcan
BACKGROUND: Heterochronic plasma exchange is widely used to investigate systemic aging; however, its pulmonary consequences particularly regarding oxidative stress, antioxidant defense, and tissue architecture have not been systematically examined. METHODS: Twenty-four-month-old male Sprague-Dawley rats received 0.5 mL of young plasma intravenously daily for 30 days, while 8-week-old rats received 0.25 mL of aged plasma. After treatment, lung tissues were analyzed histologically, biochemically, and molecularly. RESULTS: Quantitative PCR showed that young plasma markedly upregulated antioxidant defenses, with SOD and CAT expression increasing by ∼2.5-fold and 1.8-fold, respectively (p < 0.01), accompanied by higher SOD and GPX enzyme activities (p < 0.05). Additional antioxidant genes (GR, GST, TXN/TXNR) were also significantly upregulated, confirming a broad activation of the antioxidant network. In contrast, aged plasma suppressed antioxidant responses, reducing CAT activity by ∼35% (p < 0.01) and similarly decreasing other enzymes. Histological analyses revealed preserved alveolar structure, thinner septa, and reduced inflammation in old + young plasma rats, while young + old plasma transfer caused structural deterioration. Immunohistochemistry confirmed increased GPX, SOD, and CAT expression in aged rats receiving young plasma, consistent with transcriptional and protein-level activation. Moreover, heterochronic plasma exchange attenuated collagen accumulation, suggesting reduced fibrillar matrix deposition, and restored the balance between alveolar epithelial Type I (AT1) and Type II (AT2) cells, indicating improved epithelial homeostasis. Toluidine Blue staining showed decreased mast-cell density after young plasma treatment (p < 0.05), reinforcing its anti-inflammatory effect. CONCLUSIONS: Young plasma exerts regenerative and anti-inflammatory actions in the aged lung, highlighting it as a key target of systemic rejuvenation. © The Author(s) 2026. Published by Oxford University Press on behalf of the Gerontological Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please cont
Metastatic Pancreatic Neuroendocrine Tumor Mimicking Interstitial Lung Disease Diagnosed by Transbronchial Lung Biopsie: A Case Report
(NLM (Medline), 2022) Sünnetçioğlu, A.; Mermit Çilingir, B.M.; Demirbas, A.; Bayram, I.; Özgökçe, M.
Pancreatic neuroendocrine tumors (PNETs) are rare malignancies characterized by insidious onset and mostly present with metastasis at the diagnosis. Lung metastasis manifesting diffuse ground-glass opacity is a very rare pattern that is difficult to distinguish from nonmalignant pulmonary diseases. Recognition of this atypical metastases pattern and avoidance of this potential pitfall are crucial. We report a unique case of the Metastatic Pancreatic Neuroendocrine Tumor Mimicking Interstitial Lung Disease diagnosed by transbronchial lung biopsie. Keywords: Neuroendocrine Tumor, Metastasis, Groundglass opacity, interstitial lung disease.
TRPM2 Channel Involvement in the Hesperidin-Mediated Potentiation of Cisplatin’s Antitumor Action in Laryngeal Carcinoma Cells
(Multidisciplinary Digital Publishing Institute (MDPI), 2026) Çınar, R.; Yıldızhan, K.; Altiner, H.İ.; Yaǧci, T.
Cisplatin (CSP) is a first-line chemotherapeutic for laryngeal squamous cell carcinoma (LSCC), but its clinical effectiveness is limited by resistance and toxicity. Hesperidin (HESP), a citrus flavonoid, may enhance chemotherapeutic efficacy through pro-apoptotic properties. This study investigated the involvement of the transient receptor potential melastatin-2 (TRPM2) channel in the HESP-mediated potentiation of CSP-induced cytotoxicity in human laryngeal carcinoma (Hep-2) cells. Hep-2 cells were treated with CSP (25 µM), HESP (25 µM), or their combination for 24 h. The findings showed that the combined application of HESP and CSP reduced cell viability by approximately 50% (p < 0.001), which was the lowest compared to CSP alone. Western blot analysis revealed that TRPM2 protein expression was higher in the CSP+HESP group compared to the control group (p < 0.001). This synergistic treatment resulted in an increase in ROS production and a decrease in MDA levels, accompanied by a reduction in cellular GSH levels (p < 0.001). Furthermore, the combination therapy increased pro-inflammatory cytokines such as IL-1β and TNF-α (p < 0.001). Functional analyses showed that HESP treatment enhanced CSP-induced Ca2+ influx and altered mitochondrial membrane potential (p < 0.001). The pharmacological inhibition of TRPM2 with ACA and 2-APB reversed these effects, restoring redox balance and reducing cellular damage. In conclusion, HESP amplifies CSP-induced apoptosis in Hep-2 cells through TRPM2-dependent oxidative stress, Ca2+ dysregulation, and mitochondrial dysfunction. These findings identify TRPM2 as a mechanistic mediator of HESP-enhanced chemosensitivity in LSCC. © 2026 by the authors.
A Comparison of Restraint Decision Tools in Intensive Care Unit: An Observational Cross-Sectional Study
(John Wiley and Sons Inc, 2026) Dogu, Ö.; Bozkurt, R.; Ziyai, N.Y.; Doğan, H.K.; Süner, K.Ö.; Erdem, A.F.
Background: The use of structured decision-making tools is highly important in reducing subjectivity in physical restraint decisions. Aim: The aim of this study was to observationally compare the effectiveness of two decision-making tools in making physical restraint decisions. Study Design: This descriptive, cross-sectional and observational comparative study. The study included patients hospitalised in the intensive care unit (ICU). Each included patient was assessed twice-daily (morning and evening) by one observer using the Restraint Decision Tree (RDT) and another observer using the Restraint Decision Wheel (RDW). Between-group differences were examined using Mann–Whitney U and Kruskal–Wallis H tests, with post hoc comparisons presented through letter-based notations adjacent to medians. Results: In total, 105 patients were included. In the morning, patients were assessed with RDT vs. RDW as not restraint: 71% vs. 72.4%, as mitted/alternative: 20% vs. 21.9% and restraint: 9% vs. 5.7%. In the evening, patients were assessed with RDT vs. RDW as not restraint: 68.6% vs. 72.4%, as mitted/alternative: 18.1% vs. 22.9%, and restraint: 13.3% vs. 4.8%. Significant differences in total Glasgow Coma Scale scores were observed in both morning and evening assessments of the RDT and in the evening assessment of the RDW (p < 0.05). Moreover, a highly significant difference was found between prior restraint history and both morning and evening assessments of the RDT and the RDW (p < 0.001). Conclusions: Both decision-making tools can be implemented by nurses in ICUs. The patient's level of consciousness and previous history of physical restraint are critical parameters affecting the restraint situation. Relevance to Clinical Practice: The widespread use of evidence-based, validated and objective decision-making tools can support restraint decisions in ICUs. Considering factors such as delirium and level of consciousness in physical restraint decisions may promote patient-centred clinical practice. © 2026 British Association of Critical Care Nurses.
Evaluation of Left Ventricular, Left Atrial, and Right Ventricular Function in Patients With Behçet's Disease by Strain Echocardiography
(John Wiley and Sons Inc, 2026) Tan, M.; Tan, R.B.; Kaya, Y.; Batur, M.
Purpose: Behçet's disease (BD) can lead to a wide spectrum of cardiovascular manifestations. Strain echocardiography has emerged as a prominent modality in clinical practice owing to its significant prognostic value and ability to detect subclinical cardiac involvement in various cardiovascular diseases. This study was designed to evaluate left ventricular (LV), left atrium (LA), and right ventricular (RV) function in BD patients using strain echocardiography. Methods: A total of 61 participants were included in the study, comprising 27 patients with BD and 34 healthy controls. After medical histories and cardiac symptoms were obtained from all participants, basic echocardiographic parameters were recorded, and LV, LA, and RV functions were assessed using strain echocardiography. Results: No significant differences were observed between the two groups in terms of cardiac chamber dimensions and diastolic parameters. No significant difference was observed in global longitudinal strain values between the BD and control groups (p = 0.078). Similarly, no significant differences were found in right ventricular strain values. Regarding LA strain (LAS) parameters, LASr was found to be significantly lower in the BD group compared with the control group (p = 0.035). In the subgroup analysis comparing BD patients with and without uveitis, there were no statistically significant differences in LASr (p = 0.813), LAScd (p = 0.963), or LASct (p = 0.911). Conclusion: In conclusion, our study did not show any deterioration in LV or RV function in BD, but it did show deterioration in LA mechanical function. © 2026 Wiley Periodicals LLC.
Dientamoeba Fragilis Dominance in IBS and Blastocystis Sp. in Ulcerative Colitis
(Elsevier Science Inc, 2026) Cengiz, Melis; Beyhan, Yunus Emre; Kayar, Yusuf
Intestinal diseases markedly impair quality of life, with irritable bowel syndrome (IBS), ulcerative colitis (UC), and Crohn's disease (CD) representing major functional and inflammatory gastrointestinal disorders. This study aimed to determine the prevalence of Dientamoeba fragilis and other intestinal parasites in these conditions and to compare the diagnostic performance of conventional and molecular methods. A total of 80 stool samples were analyzed, including 60 from patients with IBS, UC, or CD and 20 from healthy controls. Samples were examined using direct microscopy, concentration techniques, trichrome staining (TS), and real-time polymerase chain reaction (qPCR), which was applied specifically for the detection of D. fragilis. Overall, parasites were detected in 60% of patients and 15% of controls. Infection rates were 33.3% in CD, 68.8% in UC, and 58.5% in IBS patients. D. fragilis was identified in 18.8% of UC and 22.0% of IBS cases, with significant differences observed between microscopy, TS, and qPCR in detection rates. Blastocystis sp. was found in 21.7% of patients and 5% of controls, with the highest prevalence in UC patients (37.5%). Other detected parasites included Iodamoeba b & uuml;tschlii, Endolimax nana, Entamoeba coli, Giardia intestinalis, Chilomastix mesnili, Entamoeba spp., and Cystoisospora belli. While direct microscopy showed limited sensitivity, TS improved detection moderately, and qPCR provided the highest sensitivity for D. fragilis. These findings highlight the predominance of D. fragilis in IBS and Blastocystis sp. in UC and underscore the importance of molecular methods for accurate parasitological diagnosis.
Randomized Controlled Trial on the Effects of Different Antibiotic Regimens in Preterm Premature Rupture of Membranes After 34 Weeks
(Lippincott Williams & Wilkins, 2026) Karatas Gezen, Gulcan; Karaaslan, Onur
Background: To investigate the effects of different antibiotic regimens used in the treatment of preterm premature rupture of membranes (PPROM) on maternal and fetal outcomes after 34 weeks of gestation. Methods: A total of 40 pregnant women diagnosed with PPROM between 34 and 37 weeks of gestation were enrolled in this randomized controlled trial and allocated equally into 2 groups. Group 1 received sulbactam-ampicillin, azithromycin, and amoxicillin, whereas Group-2 received ceftriaxone, clarithromycin, and amoxicillin. The primary outcome was neonatal intensive care unit (NICU) length of stay. Recorded variables included maternal age, gestational age, obstetric history, time of delivery, latency period, infection markers (white blood cell count and C-reactive protein), neonatal birth weight, Apgar scores, NICU admission, mode of delivery, and maternal complications. Statistical analyses were performed using IBM SPSS Statistics for Windows, Version 27.0 (Armonk). Results: No statistically significant differences were observed between the groups in terms of age, obstetric age, obstetric history, time of delivery, infection markers (white blood cell, C-reactive protein), and type of delivery (P > .05). The difference between the groups in terms of NICU requirement and stay duration was not statistically significant; Group 1: mean 8.3 +/- 6.7 days and Group 2: 4.3 +/- 1.9 days (P = .356). The incidence of maternal complications showed no statistically significant difference; chorioamnionitis was observed in 4 patients (20.0%) in Group 1 and in a patient (5.0%) in Group 2 (P = .141). No difference was observed type of delivery and history of PPROM. Conclusion: The 2 different antibiotic regimens administered to pregnant women diagnosed with PPROM after the 34th week of gestation demonstrated similar clinical effects in terms of maternal and neonatal outcomes. Although the differences in NICU stay duration and the number of chorioamnionitis cases were not statistically significant, we believe that these findings could reach significance in studies with larger sample sizes.
Toxic Effects of Copper and Zinc Oxide Nanoparticles on Brain Tissue Antioxidant Defense of Male Swiss Albino Mice
(Springer Nature, 2026) Temiz, Ozge; Kargin, Dicle
The aim of this study was to evaluate the general toxicity of nanoscale copper oxide (CuO NPs) and zinc oxide nanoparticles (ZnO NPs) by oral gavage in mice. In the study conducted with 42 male Swiss albino mice, CuO NPs and ZnO NPs were administered at 3 different doses of 1, 5 and 25 mg/kg/day by oral gavage method for 14 days together with the control group (n = 6). GSH and GSH dependent enzymes GST, GPx and GR enzyme activities as well as thiobarbituric acid reagent (TBARS) levels, DNA oxidative damage (8-Hydroxy-2'-deoxyguanosine; 8OHdG) and oxidative protein damage (protein carbonyl; PC) were determined in male mice brain tissue after 14 days of exposure to different doses of CuO NPs and ZnO NPs by spectrophotometric methods. There was a decrease in GSH levels and a dose-dependent increase in GST, GPx and GR enzymes in the brain tissue of male mice at CuO NPs and ZnO NPs doses. In the brain tissue of male mice exposed to CuO NPs and ZnO NPs doses for 14 days, TBARS, 8-OHdG and PC levels increased compared to the control group. According to these findings, CuO NPs and ZnO NPs were shown to have dose-dependent toxic effects, inducing oxidative damage by inducing the antioxidant system in the brain of mice, and also causing negative effects on the physiological basic structures of biomolecules such as DNA, fat and protein. Our results show that CuO nanoparticles have a more toxic effect on brain tissue than ZnO nanoparticles, therefore, neurotoxic effects were determined in a dose-dependent manner and in relation to the examined antioxidant parameters.
Point-Prevalence of Antimicrobial-Related Potential Drug-Drug Interactions in Hospitalized Older Adults: A Multicenter Study Using Lexicomp
(MDPI, 2026) Arslan, Yusuf; Gurbuz, Esra; Alkan, Sevil; Vurucu, Servan; Cicek, Yeliz; Ozkaraman, Yusuf; Celen, Mustafa Kemal
Background/Objectives: Potential drug-drug interaction (pDDI) refers to the co-administration of two or more drugs that interact with each other and may have therapeutic effects. Increasing rates of polypharmacy with age increase the risk of pDDIs in geriatric patients. This multicenter study aims to provide real-world data on the incidence of pDDI associated with antimicrobial therapy in hospitalized older adults. Methods: The study screened all hospitalized patients, including those aged 65 years and older. Using the Lexicomp (R) Drug Interaction Online Database, researchers screened for pDDIs among all medications taken by patients. Results: 663 (24.0%) aged 65 and over were included in the study. Polypharmacy was present in 64.9%, and hyperpolypharmacy was present in 10.0% of the cases. 480 (72.4%) of the cases used antimicrobial therapy. The mean total number of drugs and antimicrobials used per case was 5.86 and 1.02, respectively. A total of 372 antimicrobial-related pDDIs were detected, and at least one antimicrobial-related pDDI was identified in 202 (42%) patients receiving antimicrobials. Ciprofloxacin (73.3%), clarithromycin (58.3%), and colistin (26.3%) had the highest numbers of D-type pDDIs. The antimicrobials with the highest incidence of X-type pDDIs were metronidazole (23.6%) and clarithromycin (8.3%), respectively. The logistic analysis found a significant association between antimicrobial-related pDDIs and an increase in the number of drugs, length of hospital stays, and ID departments. Conclusions: PDDI rates associated with antimicrobials, like the high pDDI rates associated with all drugs, support the literature. Therefore, strategies should be developed to reduce the risk of pDDI when prescribing antimicrobials to geriatric patients.
Protective Role of Zingerone Against High Glucose-Induced Retinal Pigment Epithelial Cell Damage Through Modulation of the TRPM2 Channel Pathway (Vol 53, 309, 2026)
(Springer, 2026) Cig, Esmanur; Yardimci, Mahmut; Cinar, Ramazan; Yildizhan, Kenan
Turkish Real-Life Atrial Fibrillation in Clinical Practice: 2-Year Clinical Outcomes of the Traffic Study
(Kare Publ, 2026) Karabay, Can; Astarcioglu, Mehmet; Agca, Fahriye; Yilmaz, Ahmet; Akture, Gulsah; Aslan, Ahmet; Tanboga, Ibrahim
Background: Atrial fibrillation (AF) is a major public health issue associated with thromboembolism and mortality. Real-world data from Tü rkiye are limited despite expanding use of non-vitamin K antagonist oral anticoagulants (NOACs). The Turkish Real Life Atrial Fibrillation in Clinical Practice (TRAFFIC) study aimed to characterize the demographic features, risk profiles, treatment patterns, and 2-year clinical outcomes of patients with non-valvular AF (NVAF) in Tü rkiye. Methods: TRAFFIC was a national, prospective, multicenter, observational registry enrolling 1659 NVAF patients from 36 centers with 6-monthly follow-up for 24 months. Baseline data included demographics, comorbidities, CHA2DS2-VASc, HAS-BLED, AF subtype, European Heart Rhythm Association (EHRA) score, and antithrombotictherapy. Outcomes were ischemic stroke/systemic embolism (SE), major bleeding, and all-cause mortality. Predictors of mortality were evaluated using adjusted Cox regression, and associations of risk scores were explored using univariate Cox models with restricted cubic splines. Results: Median age was 70 years, 48% female, with intermediate CHA2DS2-VASc (most 2-5) and low-to-intermediate HAS-BLED scores (most 0-2). Permanent AF wasthe most common subtype (48%). Antithrombotic therapy largely reflected risk profiles, with NOACs being the dominant treatment (65%). Over 2 years, all-cause mortality was 8.9%, ischemic stroke/SE 2.4%, and major bleeding 1.3%. In adjusted analysis, age, congestive heart failure, and diabetes mellitus were independent predictors of mortality. Both CHA2DS2-VASc and HAS-BLED scores showed threshold effects for mortality and thromboembolic risk but notfor bleeding. Conclusion: TRAFFIC provides contemporary Turkish NVAF data, showing lower event rates than historical cohorts. Outcomes are comparable with international registries; persistent mortality burden highlights the needforAF care beyond anticoagulation.
Reader Comment on "Physician Vs AI Accuracy in Cath Lab Activation for STEMI-Equivalent and Mimic ECGs
(WB Saunders Co-Elsevier Inc, 2026) Kaya, Ahmet Ferhat
Association Between Hashimoto's Thyroiditis and Hemoglobin-Albumin Score and Systemic Inflammatory Index: A Nationwide Cohort Study
(Taylor & Francis Ltd, 2026) Tel, Burcin Meryem Atak; Bicer, Irem; Bavunoglu, Isil; Duzen Oflas, Nur; Sahin Tekin, Melisa; Sen, Hacer; Karaahmetoglu, Selma
ObjectivesHashimoto's thyroiditis (HT) is a T-cell mediated autoimmune disease characterized by the progressive destruction of thyroid gland. Hemoglobin-Albumin-Lymphocyte-Platelet (HALP) score and systemic inflammatory index (SII) are novel markers of inflammation. We aimed to compare HALP score and SII values of patients with HT to those in healthy control subjects in the present study.MethodsPatients diagnosed with HT and healthy volunteers (as controls) were included in the study. The SII and HALP score were calculated using the following formulas: SII = (Platelet countxNeutrophil count)/Lymphocyte count. HALP score = (HemoglobinxSerum AlbuminxLymphocyte count)/Platelet count. SII and HALP score of patients with HT and healthy controls were compared.ResultsMedian SII of the patients with HT (510 (140-3646)) was significantly higher than that of the control subjects (422 (102-2173)) (p < 0.001). Median HALP score of the HT group (47 (7-149)) was significantly lower than that of the control group (54 (10-160)) (p < 0.001). The sensitivity and specificity of SII (when higher than 452%) in detecting HT were 61% and 62%, respectively (AUC: 0.64, p < 0.001, 95%CI: 0.61-0.67). A HALP score lower than 49.5% threshold had 64% sensitivity and 57% specificity in detecting HT (AUC: 0.61, p < 0.001, 95%CI: 0.57-0.64). In logistic regression analysis (considering age, gender, eGFR, TSH, CRP, ESR, BMI), a unit increase in SII increased the risk of HT by 0.3% (p < 0.001, OR: 1.003, 95%CI: 1.002-1.004). HALP score was also an independent risk factor for HT. A unit increase in HALP score decreased the risk of HT by 2% (p < 0.001, OR: 0.977, 95%CI: 0.968-0.985).ConclusionWe recommend that, due to their inexpensive and easily assessable nature, SII and HALP score could serve as additional diagnostic tools in HT.
Meaning Reconstruction and Grief Thirteen Years After the Roboski Massacre: A Qualitative Study
(Routledge Journals, Taylor & Francis Ltd, 2026) Ayhan, Cemile Hurrem; Neimeyer, Robert A.; Tanhan, Fuat; Avci Erdal, Nursel; Yagan, Ferdi; Ozturk, Gamze; Aslangiri, Sevgi
This study explores the bereavement and meaning reconstruction experiences of family members who lost loved ones in the Roboski massacre, in which 34 civilians-mostly young-were killed in a cross-border airstrike in Turkey. Using a hermeneutic phenomenological qualitative design, three core themes emerged: (1) Traumatic Circumstances and Risk Factors, including the young age of the deceased, violent nature of death, and socioeconomic hardship compounded by limited institutional support; (2) Disrupted Meaning, stemming from injustice, impunity, and fractured trust in state authorities, and (3) Life After Loss: Bonds, Practices, and Adaptation, marked by emotional pain, disrupted health, symbolic remembrance, and reliance on cultural mourning rituals; and (3). Findings reveal how grief in the aftermath of collective violence is shaped by both individual and sociopolitical dimensions. The study underscores the need for trauma-informed, culturally responsive, and justice-oriented interventions for communities coping with mass loss.
Evaluation of Inflammatory Parameters and Ferritin as Prognostic Factors in Non-Small Cell Lung Cancer Patients Receiving Nivolumab Immunotherapy: Prognostic Inflammatory Biomarkers in NSCLC Immunotherapy
(Taylor & Francis Ltd, 2026) Beypinar, Ismail; Balcik, Onur Yazdan; Urvay, Semiha; Urun, Muslih; Ercek, Berrak; Demir, Hacer; Kurtulus, Aziz
Aim: Inflammation and immune dysfunction significantly impact cancer progression and treatment responses. This retrospective study investigated inflammatory parameters and ferritin in predicting immunotherapy response in non-small cell lung cancer (NSCLC) patients. Methods: The study included 199 patients with NSCLC who received nivolumab between 2018 and 2024 at five medical centers. Various inflammatory markers were also evaluated. Ferritin levels at diagnosis and pretreatment were also evaluated. Results: ROC curve analyses showed ferritin delta had high prognostic performance for PFS and OS, with AUC values of 0.70 and 0.73. PIV and PNI were significantly associated with PFS and OS. In Kaplan-Meier analyses, PNI was the most consistent prognostic factor. Low PNI (<= 43.5) significantly associated with shorter OS (5.0 vs. 15.0 months, p = 0.001) and shorter PFS (4.0 vs. 8.0 months, p = 0.002). High mGPS (score 2) and elevated PIV showed significant prognostic value. In multivariate Cox regression, PNI demonstrated independent prognostic significance. Objective response rate was the strongest prognostic factor for PFS (HR = 0.188, 95%CI: 0.114-0.309, p < 0.001). Conclusion: These findings highlight the prognostic value of inflammatory and nutritional markers in patients with NSCLC receiving immunotherapy. PNI demonstrated the most consistent prognostic value across multiple analytical approaches and maintained significance in the multivariate analysis.
Burnout, Intrinsic Motivation, and Emotion Regulation Profiles Among Pediatric Nurses in Turkey: A Latent Profile Analysis
(Elsevier Science Inc, 2026) Askan, Fahri; Yuruk, Emel; Todil, Tugba; Karad, Mustafa; Sata, Mehmet; Turgut, Mehmet Alperen; Celik, Baris
Objective: This study aimed to identify latent profiles based on burnout, intrinsic motivation, and emotion regulation among pediatric nurses and to examine their associations with psychological resilience, life satisfaction, depression, anxiety, and stress. Methods: A cross-sectional study involving 563 pediatric nurses was conducted between February and May 2025 in five hospitals across four provinces in Turkey. Data were collected via the Maslach Burnout Inventory, Intrinsic Motivation Scale, Emotion Regulation Scale, Satisfaction Life Scale, Brief Resilience Scale, and DepressionAnxiety-Stress Scale. Latent profile analysis was used to analyze the latent profiles of pediatric nurses, and multinomial logistic regression was used to explore the predictors of these profiles. Results: Three profiles were identified: Balanced (52.8%), Burnt-out (8.3%), and Optimistic group (38.9%). Lower resilience (OR = 0.47) and life satisfaction (OR = 0.27) increased the likelihood of being in the burnt-out profile, whereas higher resilience (OR = 1.85), higher life satisfaction (OR = 1.46), and gender (OR = 0.60) increased the likelihood of belonging to the optimistic profile. Age, depression, anxiety, and stress were not significant predictors. Conclusion: High psychological resilience and life satisfaction were associated with a greater likelihood of pediatric nurses being classified into more adaptive profiles characterized by lower burnout, higher intrinsic motivation, and more effective emotion regulation. Female nurses were more likely to belong to the burnout profile, highlighting the importance of gender-sensitive support strategies. Practical implications: Integrating profile-based assessments of burnout, intrinsic motivation, and emotion regulation into pediatric nursing practice can guide targeted individual and institutional interventions. (c) 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
Strategic Engineering of Imidazopyridine-Benzoxazole Hybrids Targeting Microtubule Dynamics: Comprehensive Inhibition of the Metastatic Cascade
(Academic Press Inc Elsevier Science, 2026) Kuzu, Burak; Cakir, Mustafa; Acikgoz, Eda
A series of twenty novel imidazopyridine-benzoxazole hybrid (Imp1-20) derivatives was designed and synthesized, and their antiproliferative activities were evaluated against MDA-MB-231 breast and DLD-1 colorectal cancer cell lines. Among them, Imp-18 and Imp-20 emerged as the most potent candidates, with low micromolar to nanomolar IC50 values and significant reductions in cancer cell adhesion and colony formation. Flow cytometry analyses demonstrated that both compounds induced apoptosis and promoted cell-cycle arrest, reflected by Sub-G1 accumulation and perturbations in G1/G0 and G2/M phases. Immunofluorescence imaging of beta-tubulin confirmed that Imp-18 and Imp-20 compromise microtubule integrity, with Imp-18 displaying stronger tubulin-disrupting activity than nocodazole. The resulting microtubule destabilization was consistent with mitotic arrest and activation of apoptotic signaling pathways. Additionally, both compounds markedly inhibited cancer cell migration, indicating an ability to impair metastatic behavior. Overall, these findings identify Imp-18 and Imp-20 as promising microtubule-targeting agents with robust anticancer potential, providing a strong basis for further mechanistic studies and structural optimization within the framework of medicinal and bioorganic chemistry.
Young Plasma Transfer Enhances Antioxidant Defense and Preserves Structural Integrity in Aged Lung Tissue
(Oxford University Press Inc, 2026) Duvenci Birben, Ozlem; Teker, Hikmet Taner; Keskin, Seda; Baba, Burcu; Kocpinar, Enver Fehim; Yildirim, Vesila; Ceylani, Taha
Background Heterochronic plasma exchange is widely used to investigate systemic aging; however, its pulmonary consequences particularly regarding oxidative stress, antioxidant defense, and tissue architecture have not been systematically examined. Methods Twenty-four-month-old male Sprague-Dawley rats received 0.5 mL of young plasma intravenously daily for 30 days, while 8-week-old rats received 0.25 mL of aged plasma. After treatment, lung tissues were analyzed histologically, biochemically, and molecularly. Results Quantitative PCR showed that young plasma markedly upregulated antioxidant defenses, with SOD and CAT expression increasing by similar to 2.5-fold and 1.8-fold, respectively (p < 0.01), accompanied by higher SOD and GPX enzyme activities (p < 0.05). Additional antioxidant genes (GR, GST, TXN/TXNR) were also significantly upregulated, confirming a broad activation of the antioxidant network. In contrast, aged plasma suppressed antioxidant responses, reducing CAT activity by similar to 35% (p < 0.01) and similarly decreasing other enzymes. Histological analyses revealed preserved alveolar structure, thinner septa, and reduced inflammation in old + young plasma rats, while young + old plasma transfer caused structural deterioration. Immunohistochemistry confirmed increased GPX, SOD, and CAT expression in aged rats receiving young plasma, consistent with transcriptional and protein-level activation. Moreover, heterochronic plasma exchange attenuated collagen accumulation, suggesting reduced fibrillar matrix deposition, and restored the balance between alveolar epithelial Type I (AT1) and Type II (AT2) cells, indicating improved epithelial homeostasis. Toluidine Blue staining showed decreased mast-cell density after young plasma treatment (p < 0.05), reinforcing its anti-inflammatory effect. Conclusions Young plasma exerts regenerative and anti-inflammatory actions in the aged lung, highlighting it as a key target of systemic rejuvenation.
The Validity and Reliability of the Modified Four Square Step Test (MFSST) in Individuals with Ankylosing Spondylitis
(Springer London Ltd, 2026) Sekeroz, Serbay; Buke, Meryem; Ilter, Server
Introduction This study aimed to examine the test-retest reliability and concurrent validity of the modified Four Square Step Test (mFSST) in individuals with ankylosing spondylitis (AS). Methods Forty-eight participants diagnosed with AS were included. The mFSST was administered twice on the same day with a 1-h seated rest to assess reliability. Concurrent validity was tested with the Timed Up and Go (TUG), Five Times Sit-to-Stand (5xSTS), and Functional Reach (FR) tests, as well as disease activity (Bath Ankylosing Spondylitis Disease Activity Index (BASDAI)) and functional status (Bath Ankylosing Spondylitis Functional Index (BASFI)) indices. Intraclass correlation coefficients (ICC), standard error of measurement (SEM), minimal detectable change (MDC95), Bland-Altman plots, and Spearman correlations were calculated. Results The mFSST demonstrated excellent test-retest reliability (ICC = 0.952), with SEM = 0.42 s and MDC95 = 1.17 s. Bland-Altman analysis showed narrow agreement limits (- 0.57 to + 1.31 s). For validity, the mFSST correlated strongly with TUG (r = 0.771, p < 0.001) and moderately with 5xSTS (r = 0.473, p < 0.001), while no significant association was found with FR (p > 0.05). Retest values confirmed these results, showing strong correlation with TUG (r = 0.823, p < 0.001), moderate with 5xSTS (r = 0.606, p < 0.001), and a weak negative correlation with FR (r = - 0.334, p < 0.05). Furthermore, both mFSST test and retest scores showed moderate positive correlations with BASDAI (r = 0.501 and r = 0.543, respectively; p < 0.001) and BASFI (r = 0.605 and r = 0.681, respectively; p < 0.001). Conclusions The mFSST is a reliable and valid tool for assessing dynamic balance in AS. Its brevity, minimal equipment needs, and interpretable error metrics support integration into clinical practice for baseline profiling and outcome monitoring. The MDC95 threshold offers clinicians a benchmark for interpreting meaningful change in rehabilitation.
SGLT2 Inhibitors Improve Subclinical Left Ventricular Systolic Function Independent of Body Mass Index in Patients with Type 2 Diabetes: A Prospective Strain Imaging Study
(Elsevier Ireland Ltd, 2026) Kumet, Omer; Polat, Fuat; Durmaz, Ozan; Can, Veysi; Kaya, Ahmet Ferhat; Ayhan, Gorkem; Ozbek, Emrah
Background: Type 2 diabetes mellitus is associated with subclinical left ventricular dysfunction. SGLT2 inhibitors demonstrate cardiovascular benefits in trials, but their effects on subclinical myocardial function and relationship to body weight in clinical practice remain unclear. This study examined global longitudinal strain (GLS) changes during SGLT2 inhibitor therapy across body mass index (BMI) categories. Methods: This prospective observational cohort enrolled 614 patients newly initiated on SGLT2 inhibitors (September 2022-May 2025), stratified by BMI: normal weight (n = 300) and overweight/obesity (n = 314). Speckle tracking echocardiography assessed GLS at baseline and 6 months. Results: Both groups showed significant GLS improvement (normal weight: -17.83 +/- 1.30% to -19.22 +/- 1.20%; overweight/obesity: -17.70 +/- 1.48% to -19.05 +/- 1.24%; both p < 0.001), with similar magnitude (p = 0.696). Overweight/obesity patients experienced modest BMI reduction (-0.66 kg/m(2), p < 0.001); normal weight remained stable. Baseline GLS strongly predicted improvement (OR = 1.553, p < 0.001), while baseline BMI showed no association (OR = 1.000, p = 0.993). Conclusion: SGLT2 inhibitor therapy was associated with similar subclinical left ventricular systolic function improvement across BMI categories. While causality cannot be established without controls and BMI imperfectly measures adiposity, findings align with randomized trial cardiovascular benefits, suggesting benefits may extend across the body weight spectrum.

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