Interaction Activity of Metal-Free Phthalocyanine Compound Bearing Tetra -(2-(N-2'-Cyanoethyl)aminoethylsulfanyl) Units with DNA

Abstract

The phthalocyanine having 4-(2-(N-2'-cyanoethyl)aminoethylsulfanyl) group had been reported earlier in the literature. In this current studying, DNA binding activity of 1Pc phthalocyanine bearing 4-(2-(N-2'-cyanoethyl)aminoethylsulfanyl units was examined spectroscopically via elctronic absoption, fluorescence titration, melting point profile, electrophoresis and viscosity methods. The interaction activity of 1Pc compound was examined at differing concentrations. UV/Vis spectrometer, viscosity, fluorescence spectroscopy and thermal melting temperature confirmed that 1Pc binds to the DNA. The Kb of 1Pc is also estimated via UV/Vis titration and Kb of 1Pc was computed as 2.1394 x 106 M-1. The Kb value demonstrated that 1Pc reacts with DNA by an intercalative mechanism. Alongside this research, the mechanism by which the compound binds to DNA was investigated by determining Tm. The Tm of DNA + 1Pc complex was identified as 74.31. This data confirmed that 1Pc binds to DNA intercalatively. All the results obtained from the used methods demonstrated that 1Pc phthalocyanine compound has an efficient DNA interaction activity and 1Pc phthalocyanine compound interacts with DNA via an intercalative mechanism. As a result, the compound may be a therapeutic agent due to its DNA interaction property. © 2025 Elsevier B.V., All rights reserved.