An Enzymatic Method for Isolating Cardiac Myocytes From Heart of Guinea-Pig Used for Single-Cell and Patch-Clamp Studies

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Date

1997

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Abstract

A method for isolating cardiac myocytes from the heart of guinea-pig is described. This method consists of perfusion of the heart with a low Ca{2+} medium and subsequent dispersion of cells with collagenase and proteolytic enzyme. More than 80% rod-shoped, viable and quiescent cells which are suitable for electrophysiological and pharmacological experiments are obtained by this method.

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Keywords

Biyoloji, Biyokimya Ve Moleküler Biyoloji, Hücre Biyolojisi

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Q3

Scopus Q

Q1

Source

Turkish Journal of Medical Sciences

Volume

27

Issue

6

Start Page

565

End Page

567

URI

https://search.trdizin.gov.tr/en/yayin/detay/43269/an-enzymatic-method-for-isolating-cardiac-myocytes-from-heart-of-guinea-pig-used-for-single-cell-and-patch-clamp-studies
 https://hdl.handle.net/20.500.14720/483

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