Purification and Characterization of Acetylcholinesterase From Sheep Liver and Inhibition by Some Painkillers
| dc.authorscopusid | 12800698100 | |
| dc.authorscopusid | 6507176660 | |
| dc.authorscopusid | 8452071500 | |
| dc.authorwosid | Türkoğlu, Vedat/A-1681-2016 | |
| dc.contributor.author | Güloglu, OF | |
| dc.contributor.author | Türkoglu, V | |
| dc.contributor.author | Çelik, I | |
| dc.date.accessioned | 2025-05-10T17:49:24Z | |
| dc.date.available | 2025-05-10T17:49:24Z | |
| dc.date.issued | 2006 | |
| dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
| dc.department-temp | Yuzuncu Yil Univ, Dept Chem, Sci & Arts Fac, Van, Turkey | en_US |
| dc.description.abstract | In this study, acetylcholinesterase (AChE; EC 3.1.1.7) was purified from sheep liver by affinity chromatography. The purification rate was found 3541.7 fold. The purification control of enzyme was done with sodium dodesilsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimal pH, ionic strength and temperature of enzyme were determined. The optimal pH and the optimum temperature were 7.5-8.5 and 35 degrees C respectively. The highest activity was seen in concentration of 0.2 M (NH4)(2)SO4 as ionic strength. On the other hand, the inhibition effects of some painkiller, (paracetamol + caffein), (neostigmin methylsulfate) and (paracetamol + propifenazon + caffein) were investigated in this study. According to results, the I-50 values are 1.27 X 10(-3), 1.02 x 10(-4) and 1.236 M respectively. Also, K-i values are determined as 1.246 x 10(-3), 4.326 x 10(-5) and 1.646 x 10(-3) mol(-1) min(-1) respectively. | en_US |
| dc.description.woscitationindex | Science Citation Index Expanded | |
| dc.identifier.endpage | 1103 | en_US |
| dc.identifier.issn | 0970-7077 | |
| dc.identifier.issn | 0975-427X | |
| dc.identifier.issue | 2 | en_US |
| dc.identifier.scopus | 2-s2.0-33645535347 | |
| dc.identifier.scopusquality | Q4 | |
| dc.identifier.startpage | 1097 | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.14720/17459 | |
| dc.identifier.volume | 18 | en_US |
| dc.identifier.wos | WOS:000236683600053 | |
| dc.identifier.wosquality | N/A | |
| dc.language.iso | en | en_US |
| dc.publisher | Asian Journal of Chemistry | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Purification | en_US |
| dc.subject | Characterisation | en_US |
| dc.subject | Inhibition | en_US |
| dc.subject | Acetylcholinesterase | en_US |
| dc.title | Purification and Characterization of Acetylcholinesterase From Sheep Liver and Inhibition by Some Painkillers | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication |