Histological and Electron Microscopic Examination of the Effect of Dexketoprofen Trometamol on Liver in Rats

Abstract

Background: The current study was performed for histological and electron microscopic examination of the effects of different doses of dexketoprofen trometamol on liver in rats. Material and Methods: Shame group consisted of rats administered 1 ml of 0.9% NaCl twice a day via intraperitoneal route, 8 mg/kg/day was used in Dexketoprofen Trometamol low-dose group, and 16 mg/kg/day was used in Dexketoprofen Trometamol high-dose group. 30 healthy Wistar albino type male rats were used in the study as animal materials. Results: The presence of TUNEL positive cells was increased with the increasing dose level of Dexketoprofen Trometamol and TUNEL positive hepatocytes distributed all over the tissue. Diffuse degeneration was determined in the liver sections of the group administered high-dose. Necrotic areas became more apparent particularly in regions close to the central vein. PCNA involvement was detected to be considerably increased compared to the shame and low-dose groups. Electron microscopic image of liver in the group administered high-dose drug showed that all hepatocytes present with highly active cell structure. Hepatocyte mitochondria were observed to be highly developed and to grow large and fuse from place to place. Granulated and smooth endoplasmic reticulum tubulus and cisternae displayed a highly-dilated appearance. Bile canaliculi were distinguished as dilated and its lumen was covered with microvilli. There were many vacuolar formation in addition to lipid droplets in the cytoplasms of ito cells. Conclusion: Dexketroprofen Trometamol drug administration was determined to increase activation particularly in parenchymal cells depending on dose and cause degeneration in liver tissue with heavy activity.