Synthesis and Characterisation of Molecularly Imprinted Microspheres for the Determination of Kanamycin

Abstract

This study aims to propose a methodology based on molecularly imprinted polymer solid phase extraction (MISPE) prior to instrumental analysis of kanamycin, a type of antibiotic. Solid phase extraction of kanamycin antibiotic, which is in the ami noglycoside class of antibiotics, from commercially purchased milk samples was carried out by molecular imprinting technique. After this process, the amount of kanamycin was determined by UV-visible spectrophotometry. This antibiotic in milk was removed by molecular imprinting method. The molecular imprinting technique was aimed to form complexes of functional monomers around a mould molecule with covalent or non-covalent interactions and then to form imprinted polymers with chemical functio n through an appropriate processing process. By removing the imprinted molecule after the process, a hollow cavity specific to the imprint ed molecule was formed in the polymeric structure. These molecularly imprinted polymers were used as an ideal material for processes such as separation, chemical determination and catalysis. Molecularly imprinted microspheres were prepared for the determinat ion of kanamycin antibiotic, the active ingredient of the drug in milk. The results obtained show that the proposed method can determine kanamycin in milk with high recovery rates and low relative standard deviation.