Vector Construction Strategies for Transformation of Wheat Plant
| dc.authorid | Kavas, Musa/0000-0001-5903-2873 | |
| dc.authorid | Baloglu, Mehmet Cengiz/0000-0003-2976-7224 | |
| dc.authorid | Oz, Mehmet Tufan/0000-0002-0042-2671 | |
| dc.authorid | Yucel, Ayse Meral/0000-0001-5548-1161 | |
| dc.authorscopusid | 36766861600 | |
| dc.authorscopusid | 25121451300 | |
| dc.authorscopusid | 55452438100 | |
| dc.authorscopusid | 59432107800 | |
| dc.authorscopusid | 36466864700 | |
| dc.authorscopusid | 25822550100 | |
| dc.authorscopusid | 6701805729 | |
| dc.authorwosid | Aydin, Gulsum/G-2752-2011 | |
| dc.authorwosid | Baloglu, Mehmet/P-2803-2019 | |
| dc.authorwosid | Battal, Abdulhamit/Jfs-6886-2023 | |
| dc.authorwosid | Kavas, Musa/K-3941-2017 | |
| dc.authorwosid | Baloglu, Mehmet Cengiz/P-7411-2016 | |
| dc.authorwosid | Oz, Mehmet Tufan/H-1640-2013 | |
| dc.authorwosid | Yucel, Ayse Meral/Aaq-3548-2021 | |
| dc.contributor.author | Baloglu, M. C. | |
| dc.contributor.author | Battal, A. | |
| dc.contributor.author | Aydin, G. | |
| dc.contributor.author | Eroglu, A. | |
| dc.contributor.author | Oz, M. T. | |
| dc.contributor.author | Kavas, M. | |
| dc.contributor.author | Yucel, M. | |
| dc.date.accessioned | 2025-05-10T17:47:52Z | |
| dc.date.available | 2025-05-10T17:47:52Z | |
| dc.date.issued | 2013 | |
| dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
| dc.department-temp | [Baloglu, M. C.] Kastamonu Univ, Dept Biol, TR-37100 Kastamonu, Turkey; [Battal, A.; Eroglu, A.; Oz, M. T.; Oktem, H. A.; Yucel, M.] Middle E Tech Univ, Dept Biol Sci, TR-06800 Ankara, Turkey; [Battal, A.; Eroglu, A.] Yuzuncu Yil Univ, Dept Biol, TR-65080 Van, Turkey; [Aydin, G.; Oz, M. T.] Selcuk Univ, Dept Biol, TR-42075 Konya, Turkey; [Kavas, M.] Ondokuz Mayis Univ, Dept Agr Biotechnol, TR-55139 Samsun, Turkey | en_US |
| dc.description | Kavas, Musa/0000-0001-5903-2873; Baloglu, Mehmet Cengiz/0000-0003-2976-7224; Oz, Mehmet Tufan/0000-0002-0042-2671; Yucel, Ayse Meral/0000-0001-5548-1161 | en_US |
| dc.description.abstract | Gene cloning and vector construction for plant genetic transformation are routine procedures in plant functional genomic studies. The availability of effective transformation vector is one of the pre-requisites for plant transformation studies. Here, we describe the construction of a series of transformation vectors through different genetic engineering techniques for wheat transformation. For this purpose, NAC-type transcription factor genes TaNAC69-1 and TtNAM-B2 were isolated from T. aestivum and T. turgidum, respectively. Then they were cloned into different types of cloning and expression vectors. Besides traditional restriction enzyme digestion and ligation method, Gateway cloning technology which is a fast and reliable alternative cloning method were used for construction of wheat transformation vectors. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium based transformation protocols for wheat plant. | en_US |
| dc.description.sponsorship | Research Fund of Middle East Technical University [BAP-08-11-DPT2002K120510]; Scientific and Technological Research Council of Turkey (TUBITAK) [108O786] | en_US |
| dc.description.sponsorship | This study was supported by the Research Fund of Middle East Technical University Grant No: BAP-08-11-DPT2002K120510 and The Scientific and Technological Research Council of Turkey (TUBITAK) Grant No: 108O786. | en_US |
| dc.description.woscitationindex | Science Citation Index Expanded | |
| dc.identifier.endpage | 912 | en_US |
| dc.identifier.issn | 1018-7081 | |
| dc.identifier.issue | 3 | en_US |
| dc.identifier.scopus | 2-s2.0-84879611440 | |
| dc.identifier.scopusquality | Q3 | |
| dc.identifier.startpage | 906 | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.14720/16909 | |
| dc.identifier.volume | 23 | en_US |
| dc.identifier.wos | WOS:000321736000037 | |
| dc.identifier.wosquality | Q3 | |
| dc.language.iso | en | en_US |
| dc.publisher | Pakistan Agricultural Scientists Forum | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Traditional Gene Cloning | en_US |
| dc.subject | Gatewaygene Cloning | en_US |
| dc.subject | Vector Construction | en_US |
| dc.subject | Wheat Transformation | en_US |
| dc.title | Vector Construction Strategies for Transformation of Wheat Plant | en_US |
| dc.type | Article | en_US |