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Polyphenol Oxidase From Malatya Apricot (Prunus Armeniaca L.)

dc.authorscopusid 35974671200
dc.authorscopusid 6507866438
dc.authorscopusid 6507332990
dc.contributor.author Arslan, O
dc.contributor.author Temur, A
dc.contributor.author Tozlu, I
dc.date.accessioned 2025-05-10T17:13:19Z
dc.date.available 2025-05-10T17:13:19Z
dc.date.issued 1998
dc.department T.C. Van Yüzüncü Yıl Üniversitesi en_US
dc.department-temp Yuzuncu Yil Univ, Sci & Art Fac, Van, Turkey; Sakarya Univ, Sci & Art Fac, Sakarya, Turkey en_US
dc.description.abstract Polyphenol oxidase (PPO) of Malatya apricot was isolated by (NH4)(2)SO4 precipitation and dialysis. PPO showed activity to catechol, L-dopa, and gallic acid. Catechol was the most suitable substrate for Malatya apricot PPO (lowest K-m value). The optimum pH for the PPO was 8.5. Heating for 40 min at 40 degrees C did not cause a significant loss of enzymic activity. The times required for 50% inactivation of activitiy at 60 and 80 degrees C were found to be 47 and 16 min, respectively. The I-50 values of inhibitors studied on PPO were determined by means of activity percentage [I] diagrams. The values were 2.7 x 10(-5), 5.03 x 10(-5), 3.62 x 10(-5), 3.68 x 10(-5), and 8.30 x 10(-4) M for sodium metabisulfite, ascorbic acid, 2-mercaptoethanol, thiourea, and salicylic acid, respectively. Ascorbic acid, 2-mercaptoethanol, sodium metabisulfite, and thiourea inhibited the reaction strongly. en_US
dc.description.woscitationindex Science Citation Index Expanded
dc.identifier.doi 10.1021/jf970599v
dc.identifier.endpage 1241 en_US
dc.identifier.issn 0021-8561
dc.identifier.issue 4 en_US
dc.identifier.scopus 2-s2.0-0000360858
dc.identifier.scopusquality Q1
dc.identifier.startpage 1239 en_US
dc.identifier.uri https://doi.org/10.1021/jf970599v
dc.identifier.uri https://hdl.handle.net/20.500.14720/8155
dc.identifier.volume 46 en_US
dc.identifier.wos WOS:000073312000004
dc.identifier.wosquality Q1
dc.language.iso en en_US
dc.publisher Amer Chemical Soc en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.subject Malatya Apricot en_US
dc.subject Prunus Armeniaca L. en_US
dc.subject Polyphenol Oxidase en_US
dc.subject Inhibitors en_US
dc.title Polyphenol Oxidase From Malatya Apricot (Prunus Armeniaca L.) en_US
dc.type Article en_US

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