An Enzymatic Method for Isolating Cardiac Myocytes From Heart of Guinea-Pig Used for Single-Cell and Patch-Clamp Studies
Abstract
A method for isolating cardiac myocytes from the heart of guinea-pig is described. This method consists of perfusion of the heart with a low Ca{2+} medium and subsequent dispersion of cells with collagenase and proteolytic enzyme. More than 80% rod-shoped, viable and quiescent cells which are suitable for electrophysiological and pharmacological experiments are obtained by this method.
Description
Keywords
Biyoloji, Biyokimya Ve Moleküler Biyoloji, Hücre Biyolojisi
Turkish CoHE Thesis Center URL
WoS Q
Q3
Scopus Q
Q1
Source
Turkish Journal of Medical Sciences
Volume
27
Issue
6
Start Page
565
End Page
567